NCALD Antibody - #DF9687

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Product: NCALD Antibody
Catalog: DF9687
Description: Rabbit polyclonal antibody to NCALD
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 22 kDa; 22kD(Calculated).
Uniprot: P61601
RRID: AB_2842883

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
NCALD Antibody detects endogenous levels of total NCALD.
RRID:
AB_2842883
Cite Format: Affinity Biosciences Cat# DF9687, RRID:AB_2842883.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MGC33870; MGC74858; NCALD; NCALD_HUMAN; Neurocalcin delta; Neurocalcin-delta;

Immunogens

Immunogen:
Uniprot:

P61601(NCALD_HUMAN) >>Visit HPA database.

Gene(ID):
NCALD(83988)
Expression:
P61601 NCALD_HUMAN:

Retina, cerebrum, cerebellum, brain stem, spinal cord, testis, ovary and small intestine.

Sequence:
MGKQNSKLRPEVMQDLLESTDFTEHEIQEWYKGFLRDCPSGHLSMEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNRDGKLSLEEFIRGAKSDPSIVRLLQCDPSSAGQF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61601 As Substrate

Site PTM Type Enzyme
K50 Ubiquitination
K102 Methylation
S106 Phosphorylation
Y129 Phosphorylation
K137 Ubiquitination
T144 Phosphorylation
K174 Ubiquitination
S175 Phosphorylation
S178 Phosphorylation

Research Backgrounds

Function:

May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions.

Tissue Specificity:

Retina, cerebrum, cerebellum, brain stem, spinal cord, testis, ovary and small intestine.

Subunit Structure:

Interacts with GUCY2D.

Family&Domains:

Belongs to the recoverin family.

Research Fields

· Organismal Systems > Sensory system > Olfactory transduction.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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