NBAS Antibody | Affinity Biosciences

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Western blot analysis NBAS using HuvEc whole cell lysates.

Product:	NBAS Antibody
Catalog:	DF9686
Description:	Rabbit polyclonal antibody to NBAS
Application:	WB
Reactivity:	Human
Prediction:	Horse, Dog
Mol.Wt.:	269 kDa; 269kD(Calculated).
Uniprot:	A2RRP1
RRID:	AB_2842882

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Horse(88%), Dog(100%)

Clonality:

Polyclonal

Specificity:

NBAS Antibody detects endogenous levels of total NBAS.

RRID:

AB_2842882
Cite Format: Affinity Biosciences Cat# DF9686, RRID:AB_2842882.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

NBAS; NBAS_HUMAN; Neuroblastoma-amplified gene protein; Neuroblastoma-amplified sequence;

Immunogens

Immunogen:

Uniprot:

A2RRP1(NBAS_HUMAN) >>Visit HPA database.

Gene(ID):

NBAS(51594)

Expression:

A2RRP1 NBAS_HUMAN:

Broadly expressed, with highest levels in heart and skeletal muscle, and lowest levels in liver, small intestine and thymus. Well expressed in retinal ganglion cells, epidermal skin cells, and leukocytes. Up-regulated together with N-myc in some neuroblastoma cell lines.

Sequence:

A2RRP1 NBAS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAPESGPALSPGTAEGEEETILYDLLVNTEWPPETEVQPRGNQKHGASFIITKAIRDRLLFLRQYIWYSPAPFLLPDGLVRLVNKQINWHLVLASNGKLLAAVQDQCVEIRSAKDDFTSIIGKCQVPKDPKPQWRRVAWSYDCTLLAYAESTGTVRVFDLMGSELFVISPASSFIGDLSYAIAGLIFLEYKASAQWSAELLVINYRGELRSYLVSVGTNQSYQESHCFSFSSHYPHGINTAIYHPGHRLLLVGGCETAEVGMSKASSCGLSAWRVLSGSPYYKQVTNGGDGVTAVPKTLGLLRMLSVKFYSRQGQEQDGIFKMSLSPDGMLLAAIHFSGKLSIWAIPSLKQQGEWGQNEQPGYDDLNPDWRLSTEKRKKIKDKESFYPLIDVNWWADSAVTLARCSGALTVSSVKTLKNLLGKSCEWFEPSPQVTATHDGGFLSLECEIKLAPKRSRLETRAGEEDEGEEDSDSDYEISAKARYFGYIKQGLYLVTEMERFAPPRKRPRTITKNYRLVSLRSTTPEELYQRKIESEEYEEALSLAHTYGLDTDLVYQRQWRKSAVNVASIQNYLSKIKKRSWVLHECLERVPENVDAAKELLQYGLKGTDLEALLAIGKGADDGRFTLPGEIDIDSISYEELSPPDEEPAKNKKEKELKKRQELLKLVNFSKLTLEQKELCRCRRKLLTYLDRLATYEEILGVPHASEQRYDAEFFKKFRNQNIVLSARTYAQESNVQALEILFTYHGSDLLPHRLAILSNFPETTSPHEYSVLLPEACFNGDSLMIIPWHEHKHRAKDWCEELACRMVVEPNLQDESEFLYAAQPELLRFRMTQLTVEKVMDWYQTRAEEIEHYARQVDCALSLIRLGMERNIPGLLVLCDNLVTLETLVYEARCDVTLTLKELQQMKDIEKLRLLMNSCSEDKYVTSAYQWMVPFLHRCEKQSPGVANELLKEYLVTLAKGDLKFPLKIFQHSKPDLQQKIIPDQDQLMAIALECIYTCERNDQLCLCYDLLECLPERGYGDKTEATTKLHDMVDQLEQILSVSELLEKHGLEKPISFVKNTQSSSEEARKLMVRLTRHTGRKQPPVSESHWRTLLQDMLTMQQNVYTCLDSDACYEIFTESLLCSSRLENIHLAGQMMHCSACSENPPAGIAHKGKPHYRVSYEKSIDLVLAASREYFNSSTNLTDSCMDLARCCLQLITDRPPAIQEELDLIQAVGCLEEFGVKILPLQVRLCPDRISLIKECISQSPTCYKQSTKLLGLAELLRVAGENPEERRGQVLILLVEQALRFHDYKAASMHCQELMATGYPKSWDVCSQLGQSEGYQDLATRQELMAFALTHCPPSSIELLLAASSSLQTEILYQRVNFQIHHEGGENISASPLTSKAVQEDEVGVPGSNSADLLRWTTATTMKVLSNTTTTTKAVLQAVSDGQWWKKSLTYLRPLQGQKCGGAYQIGTTANEDLEKQGCHPFYESVISNPFVAESEGTYDTYQHVPVESFAEVLLRTGKLAEAKNKGEVFPTTEVLLQLASEALPNDMTLALAYLLALPQVLDANRCFEKQSPSALSLQLAAYYYSLQIYARLAPCFRDKCHPLYRADPKELIKMVTRHVTRHEHEAWPEDLISLTKQLHCYNERLLDFTQAQILQGLRKGVDVQRFTADDQYKRETILGLAETLEESVYSIAISLAQRYSVSRWEVFMTHLEFLFTDSGLSTLEIENRAQDLHLFETLKTDPEAFHQHMVKYIYPTIGGFDHERLQYYFTLLENCGCADLGNCAIKPETHIRLLKKFKVVASGLNYKKLTDENMSPLEALEPVLSSQNILSISKLVPKIPEKDGQMLSPSSLYTIWLQKLFWTGDPHLIKQVPGSSPEWLHAYDVCMKYFDRLHPGDLITVVDAVTFSPKAVTKLSVEARKEMTRKAIKTVKHFIEKPRKRNSEDEAQEAKDSKVTYADTLNHLEKSLAHLETLSHSFILSLKNSEQETLQKYSHLYDLSRSEKEKLHDEAVAICLDGQPLAMIQQLLEVAVGPLDISPKDIVQSAIMKIISALSGGSADLGGPRDPLKVLEGVVAAVHASVDKGEELVSPEDLLEWLRPFCADDAWPVRPRIHVLQILGQSFHLTEEDSKLLVFFRTEAILKASWPQRQVDIADIENEENRYCLFMELLESSHHEAEFQHLVLLLQAWPPMKSEYVITNNPWVRLATVMLTRCTMENKEGLGNEVLKMCRSLYNTKQMLPAEGVKELCLLLLNQSLLLPSLKLLLESRDEHLHEMALEQITAVTTVNDSNCDQELLSLLLDAKLLVKCVSTPFYPRIVDHLLASLQQGRWDAEELGRHLREAGHEAEAGSLLLAVRGTHQAFRTFSTALRAAQHWV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Horse

Pig

Rabbit

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - A2RRP1 As Substrate

A2RRP1

Site	PTM Type	Source
S11	Phosphorylation	Uniprot
K45	Ubiquitination	Uniprot
K115	Ubiquitination	Uniprot
K265	Ubiquitination	Uniprot
K284	Ubiquitination	Uniprot
T294	Phosphorylation	Uniprot
S307	Phosphorylation	Uniprot
K416	Ubiquitination	Uniprot
K419	Ubiquitination	Uniprot
K424	Ubiquitination	Uniprot
S473	Phosphorylation	Uniprot
S475	Phosphorylation	Uniprot
Y477	Phosphorylation	Uniprot
S480	Phosphorylation	Uniprot
K482	Ubiquitination	Uniprot
Y485	Phosphorylation	Uniprot
Y488	Phosphorylation	Uniprot
K490	Ubiquitination	Uniprot
Y494	Phosphorylation	Uniprot
T497	Phosphorylation	Uniprot
T511	Phosphorylation	Uniprot
T525	Phosphorylation	Uniprot
K533	Ubiquitination	Uniprot
Y539	Phosphorylation	Uniprot
Y549	Phosphorylation	Uniprot
Y557	Phosphorylation	Uniprot
K563	Ubiquitination	Uniprot
S564	Phosphorylation	Uniprot
K577	Ubiquitination	Uniprot
K600	Ubiquitination	Uniprot
K620	Ubiquitination	Uniprot
Y640	Phosphorylation	Uniprot
K673	Ubiquitination	Uniprot
K679	Ubiquitination	Uniprot
K687	Ubiquitination	Uniprot
K799	Ubiquitination	Uniprot
K841	Ubiquitination	Uniprot
K944	Ubiquitination	Uniprot
K955	Ubiquitination	Uniprot
K971	Ubiquitination	Uniprot
K977	Ubiquitination	Uniprot
T1027	Phosphorylation	Uniprot
T1030	Phosphorylation	Uniprot
K1052	Ubiquitination	Uniprot
K1057	Acetylation	Uniprot
K1057	Ubiquitination	Uniprot
K1063	Ubiquitination	Uniprot
K1086	Ubiquitination	Uniprot
S1145	Phosphorylation	Uniprot
K1169	Ubiquitination	Uniprot
S1243	Phosphorylation	Uniprot
K1246	Ubiquitination	Uniprot
K1257	Ubiquitination	Uniprot
S1259	Phosphorylation	Uniprot
T1260	Phosphorylation	Uniprot
K1261	Acetylation	Uniprot
K1261	Ubiquitination	Uniprot
K1314	Ubiquitination	Uniprot
S1382	Phosphorylation	Uniprot
S1388	Phosphorylation	Uniprot
K1440	Ubiquitination	Uniprot
S1441	Phosphorylation	Uniprot
T1443	Phosphorylation	Uniprot
K1452	Ubiquitination	Uniprot
K1603	Ubiquitination	Uniprot
K1667	Ubiquitination	Uniprot
K1801	Ubiquitination	Uniprot
K1832	Ubiquitination	Uniprot
S1842	Phosphorylation	Uniprot
S1869	Phosphorylation	Uniprot
S1870	Phosphorylation	Uniprot
Y1877	Phosphorylation	Uniprot
Y1883	Phosphorylation	Uniprot
T1894	Phosphorylation	Uniprot
S1902	Phosphorylation	Uniprot
K1908	Ubiquitination	Uniprot
K1931	Acetylation	Uniprot
K1948	Ubiquitination	Uniprot
K1986	Ubiquitination	Uniprot
K1998	Ubiquitination	Uniprot
S2052	Phosphorylation	Uniprot
K2213	Ubiquitination	Uniprot

Research Backgrounds

A2RRP1_NBAS_HUMAN

Function:

Involved in Golgi-to-endoplasmic reticulum (ER) retrograde transport; the function is proposed to depend on its association in the NRZ complex which is believed to play a role in SNARE assembly at the ER.

Subcellular Location:

Cytoplasm. Endoplasmic reticulum. Endoplasmic reticulum membrane>Peripheral membrane protein.

Tissue Specificity:

Subunit Structure:

Component of the NRZ complex composed of NBAS, ZW10 and RINT1/TIP20L; NRZ associates with SNAREs STX18, USE1L, BNIP1/SEC20L and SEC22B (the assembly has been described as syntaxin 18 complex); links NRZ to SNARE USE1L.

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NBAS Antibody - #DF9686