MYBPC2 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of MYBPC2 using mouse liver lysates.

DF9649 at 1/100 staining mouse muscle tissue by IHC-P.

Product:	MYBPC2 Antibody
Catalog:	DF9649
Description:	Rabbit polyclonal antibody to MYBPC2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	130~150kDa; 128kD(Calculated).
Uniprot:	Q14324
RRID:	AB_2842845

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%), Chicken(88%)

Clonality:

Polyclonal

Specificity:

MYBPC2 Antibody detects endogenous levels of total MYBPC2.

RRID:

AB_2842845
Cite Format: Affinity Biosciences Cat# DF9649, RRID:AB_2842845.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C protein, skeletal muscle fast isoform; C-protein; Fast MyBP C; Fast MyBP-C; MGC163408; MYBPC; Mybpc2; MYBPCF; Myosin binding protein C, fast type; Myosin-binding protein C, fast-type; MYPC2_HUMAN; skeletal muscle fast isoform;

Immunogens

Immunogen:

Uniprot:

Q14324(MYPC2_HUMAN) >>Visit HPA database.

Gene(ID):

MYBPC2(4606)

Sequence:

Q14324 MYPC2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPEAKPAAKKAPKGKDAPKGAPKEAPPKEAPAEAPKEAPPEDQSPTAEEPTGVFLKKPDSVSVETGKDAVVVAKVNGKELPDKPTIKWFKGKWLELGSKSGARFSFKESHNSASNVYTVELHIGKVVLGDRGYYRLEVKAKDTCDSCGFNIDVEAPRQDASGQSLESFKRTSEKKSDTAGELDFSGLLKKREVVEEEKKKKKKDDDDLGIPPEIWELLKGAKKSEYEKIAFQYGITDLRGMLKRLKKAKVEVKKSAAFTKKLDPAYQVDRGNKIKLMVEISDPDLTLKWFKNGQEIKPSSKYVFENVGKKRILTINKCTLADDAAYEVAVKDEKCFTELFVKEPPVLIVTPLEDQQVFVGDRVEMAVEVSEEGAQVMWMKDGVELTREDSFKARYRFKKDGKRHILIFSDVVQEDRGRYQVITNGGQCEAELIVEEKQLEVLQDIADLTVKASEQAVFKCEVSDEKVTGKWYKNGVEVRPSKRITISHVGRFHKLVIDDVRPEDEGDYTFVPDGYALSLSAKLNFLEIKVEYVPKQEPPKIHLDCSGKTSENAIVVVAGNKLRLDVSITGEPPPVATWLKGDEVFTTTEGRTRIEKRVDCSSFVIESAQREDEGRYTIKVTNPVGEDVASIFLQVVDVPDPPEAVRITSVGEDWAILVWEPPMYDGGKPVTGYLVERKKKGSQRWMKLNFEVFTETTYESTKMIEGILYEMRVFAVNAIGVSQPSMNTKPFMPIAPTSEPLHLIVEDVTDTTTTLKWRPPNRIGAGGIDGYLVEYCLEGSEEWVPANTEPVERCGFTVKNLPTGARILFRVVGVNIAGRSEPATLAQPVTIREIAEPPKIRLPRHLRQTYIRKVGEQLNLVVPFQGKPRPQVVWTKGGAPLDTSRVHVRTSDFDTVFFVRQAARSDSGEYELSVQIENMKDTATIRIRVVEKAGPPINVMVKEVWGTNALVEWQAPKDDGNSEIMGYFVQKADKKTMEWFNVYERNRHTSCTVSDLIVGNEYYFRVYTENICGLSDSPGVSKNTARILKTGITFKPFEYKEHDFRMAPKFLTPLIDRVVVAGYSAALNCAVRGHPKPKVVWMKNKMEIREDPKFLITNYQGVLTLNIRRPSPFDAGTYTCRAVNELGEALAECKLEVRVPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Horse

Chicken

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14324 As Substrate

Q14324

Site	PTM Type	Source
S44	Phosphorylation	Uniprot
T46	Phosphorylation	Uniprot
S98	Phosphorylation	Uniprot
S105	Phosphorylation	Uniprot
K223	Acetylation	Uniprot
K228	Acetylation	Uniprot
K249	Acetylation	Uniprot
S409	Phosphorylation	Uniprot
Y472	Phosphorylation	Uniprot
S481	Phosphorylation	Uniprot
S722	Phosphorylation	Uniprot
S820	Phosphorylation	Uniprot
T849	Phosphorylation	Uniprot
S962	Phosphorylation	Uniprot
Y967	Phosphorylation	Uniprot
Y1003	Phosphorylation	Uniprot
Y1007	Phosphorylation	Uniprot
S1015	Phosphorylation	Uniprot
K1078	Ubiquitination	Uniprot
K1085	Methylation	Uniprot
Y1099	Phosphorylation	Uniprot
T1104	Phosphorylation	Uniprot

Research Backgrounds

Q14324_MYPC2_HUMAN

Function:

Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.

Family&Domains:

Belongs to the immunoglobulin superfamily. MyBP family.

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MYBPC2 Antibody - #DF9649