MYH8 Antibody - #DF9648

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Product: MYH8 Antibody
Catalog: DF9648
Description: Rabbit polyclonal antibody to MYH8
Application: WB
Reactivity: Human
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 223kDa; 223kD(Calculated).
Uniprot: P13535
RRID: AB_2842844

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(92%), Horse(100%), Rabbit(100%), Dog(83%)
Clonality:
Polyclonal
Specificity:
MYH8 Antibody detects endogenous levels of total MYH8.
RRID:
AB_2842844
Cite Format: Affinity Biosciences Cat# DF9648, RRID:AB_2842844.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DA7; Fetal myosin heavy chain; gtMHC F; MYH8; MYH8_HUMAN; MyHC peri; MyHC pn; MyHC-perinatal; Myosin heavy chain 8; Myosin heavy chain; Myosin heavy chain, skeletal muscle, perinatal; Myosin, heavy chain 8, skeletal muscle, perinatal; Myosin, heavy polypeptide 8, skeletal muscle, perinatal; Myosin-8; perinatal; skeletal muscle;

Immunogens

Immunogen:
Uniprot:

P13535(MYH8_HUMAN) >>Visit HPA database.

Gene(ID):
MYH8(4626)
Sequence:
MSASSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVAEPKESYVKSTIQSKEGGKVTVKTEGGATLTVREDQVFPMNPPKYDKIEDMAMMTHLHEPGVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYKPEVVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKDESGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQITSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIDILGFTPEEKVSIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNAVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKSANFQKPKVVKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNDTVVGLYQKSAMKTLASLFSTYASAEADSSAKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQIITRTQAVCRGFLMRVEYQKMLQRREALFCIQYNVRAFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQSEADSLADAEERCEQLIKNKIQLEAKIKEVTERAEEEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLSKEKKALQETHQQTLDDLQAEEDKVNILTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESTMDMENDKQQLDEKLEKKEFEISNLISKIEDEQAVEIQLQKKIKELQARIEELGEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQVELNKKREAEFQKLRRDLEEATLQHEAMVAALRKKHADSMAELGEQIDNLQRVKQKLEKEKSELKMETDDLSSNAEAISKAKGNLEKMCRSLEDQVSELKTKEEEQQRLINDLTAQRARLQTEAGEYSRQLDEKDALVSQLSRSKQASTQQIEELKHQLEEETKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERSNAACAALDKKQRNFDKVLSEWKQKYEETQAELEASQKESRSLSTELFKVKNVYEESLDQLETLRRENKNLQQEISDLTEQIAEGGKQIHELEKIKKQVEQEKCEIQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQLKRNHTRVVETMQSTLDAEIRSRNDALRVKKKMEGDLNEMEIQLNHANRLAAESLRNYRNTQGILKETQLHLDDALRGQEDLKEQLAIVERRANLLQAEIEELWATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLENDVSQLQSEVEEVIQESRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVENEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQAKVKSYKRQAEEAEEQSNANLSKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKISAE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Pig
92
Dog
83
Bovine
75
Sheep
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13535 As Substrate

Site PTM Type Enzyme
T63 Phosphorylation
T73 Phosphorylation
Y165 Phosphorylation
T170 Phosphorylation
Y197 Phosphorylation
T200 Phosphorylation
K249 Ubiquitination
T258 Phosphorylation
S263 Phosphorylation
Y269 Phosphorylation
K273 Ubiquitination
S351 Phosphorylation
Y353 Phosphorylation
T356 Phosphorylation
Y362 Phosphorylation
T381 Phosphorylation
Y389 Phosphorylation
Y404 Phosphorylation
Y413 Phosphorylation
T415 Phosphorylation
K416 Acetylation
Y424 Phosphorylation
K505 Acetylation
Y556 Phosphorylation
Y591 Phosphorylation
T663 Phosphorylation
S731 Phosphorylation
S741 Phosphorylation
K743 Acetylation
S745 Phosphorylation
T791 Phosphorylation
Y819 Phosphorylation
K834 Acetylation
K844 Acetylation
T854 Phosphorylation
T862 Phosphorylation
K874 Acetylation
S951 Phosphorylation
K954 Acetylation
K955 Acetylation
T963 Phosphorylation
K979 Acetylation
T982 Phosphorylation
T991 Phosphorylation
K994 Acetylation
K1000 Ubiquitination
S1040 Phosphorylation
K1045 Acetylation
S1140 Phosphorylation
S1143 Phosphorylation
K1169 Ubiquitination
K1176 Ubiquitination
K1222 Ubiquitination
S1236 Phosphorylation
T1277 Phosphorylation
S1302 Phosphorylation
S1338 Phosphorylation
S1369 Phosphorylation
T1376 Phosphorylation
Y1378 Phosphorylation
T1380 Phosphorylation
T1386 Phosphorylation
K1393 Acetylation
K1394 Acetylation
K1395 Acetylation
T1420 Phosphorylation
Y1463 Phosphorylation
T1466 Phosphorylation
S1477 Phosphorylation
S1479 Phosphorylation
S1481 Phosphorylation
T1482 Phosphorylation
S1513 Phosphorylation
T1516 Phosphorylation
S1573 Phosphorylation
S1602 Phosphorylation
T1603 Phosphorylation
Y1646 Phosphorylation
T1649 Phosphorylation
S1701 Phosphorylation
S1713 Phosphorylation
T1721 Phosphorylation
T1724 Phosphorylation
S1725 Phosphorylation
T1729 Phosphorylation
K1730 Acetylation
T1763 Phosphorylation
K1773 Acetylation
T1854 Phosphorylation
Y1855 Phosphorylation
K1862 Acetylation
S1918 Phosphorylation
K1933 Acetylation

Research Backgrounds

Function:

Muscle contraction.

Subcellular Location:

Cytoplasm>Myofibril.
Note: Thick filaments of the myofibrils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Family&Domains:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

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