MIA3 Antibody - #DF9622

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Product: MIA3 Antibody
Catalog: DF9622
Description: Rabbit polyclonal antibody to MIA3
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Dog, Chicken
Mol.Wt.: 214kDa; 214kD(Calculated).
Uniprot: Q5JRA6
RRID: AB_2842818

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Dog(83%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
MIA3 Antibody detects endogenous levels of total MIA3.
RRID:
AB_2842818
Cite Format: Affinity Biosciences Cat# DF9622, RRID:AB_2842818.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARNT; C219 reactive peptide; C219-reactive peptide; D320; Melanoma inhibitory activity family, member 3; Melanoma inhibitory activity protein 3; Mia3; MIA3_HUMAN; RP11-378J18.4; TANGO; Transport and Golgi organization protein 1; UNQ6077;

Immunogens

Immunogen:
Uniprot:

Q5JRA6(TGO1_HUMAN) >>Visit HPA database.

Gene(ID):
MIA3(375056)
Expression:
Q5JRA6 TGO1_HUMAN:

Broadly expressed, except in bone marrow and peripheral blood mononuclear cells. Down-regulated in melanoma tissue.

Sequence:
MAAAPGLLVWLLVLRLPWRVPGQLDPSTGRRFSEHKLCADDECSMLMYRGEALEDFTGPDCRFVNFKKGDPVYVYYKLARGWPEVWAGSVGRTFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFHNYNVEELLGFLELYNSAATDSEKAVEKTLQDMEKNPELSKEREPEPEPVEANSEESDSVFSENTEDLQEQFTTQKHHSHANSQANHAQGEQASFESFEEMLQDKLKVPESENNKTSNSSQVSNEQDKIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDTEYYAVGKEDEENQEDFDELPLLTFTDGEDMKTPAKSGVEKYPTDKEQNSNEEDKVQLTVPPGIKNDDKNILTTWGDTIFSIVTGGEETRDTMDLESSSSEEEKEDDDDALVPDSKQGKPQSATDYSDPDNVDDGLFIVDIPKTNNDKEVNAEHHIKGKGRGVQESKRGLVQDKTELEDENQEGMTVHSSVHSNNLNSMPAAEKGKDTLKSAYDDTENDLKGAAIHISKGMLHEEKPGEQILEGGSESESAQKAAGNQMNDRKIQQESLGSAPLMGDDHPNASRDSVEGDALVNGAKLHTLSVEHQREELKEELVLKTQNQPRFSSPDEIDLPRELEDEVPILGRNLPWQQERDVAATASKQMSEKIRLSEGEAKEDSLDEEFFHHKAMQGTEVGQTDQTDSTGGPAFLSKVEEDDYPSEELLEDENAINAKRSKEKNPGNQGRQFDVNLQVPDRAVLGTIHPDPEIEESKQETSMILDSEKTSETAAKGVNTGGREPNTMVEKERPLADKKAQRPFERSDFSDSIKIQTPELGEVFQNKDSDYLKNDNPEEHLKTSGLAGEPEGELSKEDHENTEKYMGTESQGSAAAEPEDDSFHWTPHTSVEPGHSDKREDLLIISSFFKEQQSLQRFQKYFNVHELEALLQEMSSKLKSAQQESLPYNMEKVLDKVFRASESQILSIAEKMLDTRVAENRDLGMNENNIFEEAAVLDDIQDLIYFVRYKHSTAEETATLVMAPPLEEGLGGAMEEMQPLHEDNFSREKTAELNVQVPEEPTHLDQRVIGDTHASEVSQKPNTEKDLDPGPVTTEDTPMDAIDANKQPETAAEEPASVTPLENAILLIYSFMFYLTKSLVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLISENKKSIEKLKDVISMNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Chicken
100
Dog
83
Pig
67
Bovine
67
Sheep
67
Rabbit
60
Zebrafish
57
Horse
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5JRA6 As Substrate

Site PTM Type Enzyme
S33 Phosphorylation
S44 Phosphorylation
T57 Phosphorylation
Y73 Phosphorylation
K156 Ubiquitination
T161 O-Glycosylation
S186 Phosphorylation
S226 Phosphorylation
S229 Phosphorylation
T248 Phosphorylation
K278 Ubiquitination
S281 Phosphorylation
T282 Phosphorylation
S288 Phosphorylation
T293 Phosphorylation
Y350 Phosphorylation
S358 Phosphorylation
S405 Phosphorylation
S407 Phosphorylation
S408 Phosphorylation
K451 Acetylation
T524 Phosphorylation
N589 N-Glycosylation
S591 Phosphorylation
S727 Phosphorylation
K740 Ubiquitination
T768 O-Glycosylation
S788 Phosphorylation
T838 O-Glycosylation
S876 Phosphorylation
Y969 Phosphorylation
T1040 O-Glycosylation
S1067 Phosphorylation
T1083 O-Glycosylation
T1093 O-Glycosylation
S1099 O-Glycosylation
T1104 O-Glycosylation
K1158 Acetylation
S1159 Phosphorylation
T1163 Phosphorylation
Y1175 Phosphorylation
K1219 Ubiquitination
K1221 Ubiquitination
K1233 Ubiquitination
K1240 Ubiquitination
T1251 Phosphorylation
K1253 Ubiquitination
K1274 Ubiquitination
K1284 Ubiquitination
S1431 Phosphorylation
K1472 Ubiquitination
K1477 Ubiquitination
S1483 Phosphorylation
T1484 Phosphorylation
K1485 Ubiquitination
K1506 Ubiquitination
T1515 Phosphorylation
S1539 Phosphorylation
S1553 Phosphorylation
Y1569 Phosphorylation
K1597 Ubiquitination
S1666 Phosphorylation
S1670 Phosphorylation
S1673 Phosphorylation
S1678 Phosphorylation
T1682 Phosphorylation
S1691 Phosphorylation
T1693 Phosphorylation
S1702 Phosphorylation
S1706 Phosphorylation
K1723 Ubiquitination
S1725 Phosphorylation
S1727 Phosphorylation
T1733 Phosphorylation
T1735 Phosphorylation
S1739 Phosphorylation
S1740 Phosphorylation
S1741 Phosphorylation
S1744 Phosphorylation
S1745 Phosphorylation
R1809 Methylation
S1847 Phosphorylation
R1859 Methylation
R1877 Methylation
S1906 Phosphorylation

Research Backgrounds

Function:

Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum. Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein.
Note: Localizes at endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). SEC16A is required for its proper localization to ERES. After loading of COL7A1 into transport carriers, it is not incorporated into COPII carriers and remains in the endoplasmic reticulum membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Broadly expressed, except in bone marrow and peripheral blood mononuclear cells. Down-regulated in melanoma tissue.

Subunit Structure:

Interacts with MIA2. Interacts (via SH3 domain) with COL7A1. Interacts with the COPII coat subunits SEC23A, SEC23B and maybe SEC24C. May interact with APOB and MIA2. Isoform 1 and isoform 4 interact with SEC16A.

Family&Domains:

The proline-rich domain (PRD) contains repeated PPP motifs. A single PPP motif is necessary and sufficient to mediate interaction with the COPII coat subunits SEC23A and SEC23B.

Although 2 transmembrane domains are predicted, PubMed:19269366 showed that it only contains one transmembrane domain. The other predicted transmembrane region is probably a hairpin-type region embedded into the membrane, which does not cross the membrane. It is unclear which of the 2 predicted transmembrane regions is the transmembrane or the hairpin-type region.

Belongs to the MIA/OTOR family. Tango1 subfamily.

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