MED9 Antibody | Affinity Biosciences

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Western blot analysis of MED9 using Jurkat whole cell lysates.

Western blot analysis of extracts from Myeloma cells, using MED9 Antibody.

Product:	MED9 Antibody
Catalog:	DF9620
Description:	Rabbit polyclonal antibody to MED9
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	16 kDa; 16kD(Calculated).
Uniprot:	Q9NWA0
RRID:	AB_2842816

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(100%), Horse(83%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

MED9 Antibody detects endogenous levels of total MED9.

RRID:

AB_2842816
Cite Format: Affinity Biosciences Cat# DF9620, RRID:AB_2842816.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ10193; MED25; MED9; Mediator complex subunit 9; mediator of RNA polymerase II transcription subunit 9; mediator of RNA polymerase II transcription, subunit 9 homolog; Mediator subunit 25; MGC138234;

Immunogens

Immunogen:

Uniprot:

Q9NWA0(MED9_HUMAN) >>Visit HPA database.

Gene(ID):

MED9(55090)

Sequence:

Q9NWA0 MED9_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASAGVAAGRQAEDVLPPTSDQPLPDTKPLPPPQPPPVPAPQPQQSPAPRPQSPARAREEENYSFLPLVHNIIKCMDKDSPEVHQDLNALKSKFQEMRKLISTMPGIHLSPEQQQQQLQSLREQVRTKNELLQKYKSLCMFEIPKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Dog

100

Chicken

100

Horse

Xenopus

Pig

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NWA0 As Substrate

Q9NWA0

Site	PTM Type	Source
A2	Acetylation	Uniprot
S3	Phosphorylation	Uniprot
T19	Phosphorylation	Uniprot
S20	Phosphorylation	Uniprot
S46	Phosphorylation	Uniprot
S53	Phosphorylation	Uniprot
Y63	Phosphorylation	Uniprot
S64	Phosphorylation	Uniprot
S80	Phosphorylation	Uniprot
K91	Ubiquitination	Uniprot
K99	Ubiquitination	Uniprot
S110	Phosphorylation	Uniprot
K128	Sumoylation	Uniprot
K134	Ubiquitination	Uniprot
K136	Ubiquitination	Uniprot
K145	Ubiquitination	Uniprot

Research Backgrounds

Q9NWA0_MED9_HUMAN

Function:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.

Family&Domains:

Belongs to the Mediator complex subunit 9 family.

Restrictive clause

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For Research Use Only.
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MED9 Antibody - #DF9620