MED29 Antibody - #DF9616
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9616, RRID:AB_2842812.
Fold/Unfold
DKFZp434H247; Intersex like protein; IXL; Mediator complex subunit 29; Mediator of RNA polymerase II transcription subunit 29;
Immunogens
- Q9NX70 MED29_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASQQQASAASSAAGVSGPSSAGGPGPQQQPQPPAQLVGPAQSGLLQQQQQDFDPVQRYKMLIPQLKESLQTLMKVAAQNLIQNTNIDNGQKSSDGPIQRFDKCLEEFYALCDQLELCLRLAHECLSQSCDSAKHSPTLVPTATKPDAVQPDSLPYPQYLAVIKAQISCAKDIHTALLDCANKVTGKTPAPPAGPGGTL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NX70 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| T176 | Phosphorylation | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| T186 | Phosphorylation | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| T199 | Phosphorylation | Uniprot |
Research Backgrounds
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Nucleus.
Widely expressed in embryo and adult.
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Associates with the MED18/MED20 heteromer.
Belongs to the Mediator complex subunit 29 family.
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