MED15 Antibody - #DF9613
| Product: | MED15 Antibody |
| Catalog: | DF9613 |
| Description: | Rabbit polyclonal antibody to MED15 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 87kDa; 87kD(Calculated). |
| Uniprot: | Q96RN5 |
| RRID: | AB_2842809 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9613, RRID:AB_2842809.
Fold/Unfold
Activator recruited cofactor 105 kD; Activator-recruited cofactor 105 kDa component; ARC105; CAG7A; CTG repeat protein 7a; CTG7A; Mediator complex subunit 15; Mediator of RNA polymerase II transcription subunit 15; PC2 glutamine rich associated protein; PC2 glutamine/Q-rich-associated protein; Positive cofactor 2 glutamine/Q-rich-associated protein; TIG-1; TIG1; TNRC7; TPA inducible gene 1; TPA inducible protein; TPA-inducible gene 1 protein; Trinucleotide repeat containing 7; Trinucleotide repeat containing gene 7 protein;
Immunogens
Expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas, blood leukocyte and placenta. However, the level of expression varied, with highest expression in the placenta and peripheral blood and lowest in the pancreas and kidney.
- Q96RN5 MED15_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVSGQETDWRSTAFRQKLVSQIEDAMRKAGVAHSKSSKDMESHVFLKAKTRDEYLSLVARLIIHFRDIHNKKSQASVSDPMNALQSLTGGPAAGAAGIGMPPRGPGQSLGGMGSLGAMGQPMSLSGQPPPGTSGMAPHSMAVVSTATPQTQLQLQQVALQQQQQQQQFQQQQQAALQQQQQQQQQQQFQAQQSAMQQQFQAVVQQQQQLQQQQQQQQHLIKLHHQNQQQIQQQQQQLQRIAQLQLQQQQQQQQQQQQQQQQALQAQPPIQQPPMQQPQPPPSQALPQQLQQMHHTQHHQPPPQPQQPPVAQNQPSQLPPQSQTQPLVSQAQALPGQMLYTQPPLKFVRAPMVVQQPPVQPQVQQQQTAVQTAQAAQMVAPGVQMITEALAQGGMHIRARFPPTTAVSAIPSSSIPLGRQPMAQVSQSSLPMLSSPSPGQQVQTPQSMPPPPQPSPQPGQPSSQPNSNVSSGPAPSPSSFLPSPSPQPSQSPVTARTPQNFSVPSPGPLNTPVNPSSVMSPAGSSQAEEQQYLDKLKQLSKYIEPLRRMINKIDKNEDRKKDLSKMKSLLDILTDPSKRCPLKTLQKCEIALEKLKNDMAVPTPPPPPVPPTKQQYLCQPLLDAVLANIRSPVFNHSLYRTFVPAMTAIHGPPITAPVVCTRKRRLEDDERQSIPSVLQGEVARLDPKFLVNLDPSHCSNNGTVHLICKLDDKDLPSVPPLELSVPADYPAQSPLWIDRQWQYDANPFLQSVHRCMTSRLLQLPDKHSVTALLNTWAQSVHQACLSAA
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96RN5 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K18 | Ubiquitination | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| Y532 | Phosphorylation | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| S568 | Phosphorylation | Uniprot | |
| K578 | Acetylation | Uniprot | |
| K578 | Ubiquitination | Uniprot | |
| K583 | Ubiquitination | Uniprot | |
| T603 | Phosphorylation | Uniprot | |
| T612 | Phosphorylation | Uniprot | |
| S673 | Phosphorylation | Uniprot | |
| S676 | Phosphorylation | Uniprot |
Research Backgrounds
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.
Ubiquitinated by TRIM11, leading to proteasomal degradation.
Cytoplasm. Nucleus.
Expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas, blood leukocyte and placenta. However, the level of expression varied, with highest expression in the placenta and peripheral blood and lowest in the pancreas and kidney.
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with SMAD2, SMAD3, SREBF1 and SREBF2. Interacts with WWTR1. Interacts with TRIM11.
Belongs to the Mediator complex subunit 15 family.
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