Product: ACSL1 Antibody
Catalog: DF9605
Description: Rabbit polyclonal antibody to ACSL1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 78 kDa; 78kD(Calculated).
Uniprot: P33121
RRID: AB_2842801

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(91%), Horse(100%), Sheep(100%), Dog(92%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
ACSL1 Antibody detects endogenous levels of total ACSL1.
RRID:
AB_2842801
Cite Format: Affinity Biosciences Cat# DF9605, RRID:AB_2842801.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACS 1; ACS1; ACSL 1; ACSL1; ACSL1_HUMAN; Acyl CoA synthetase 1; Acyl CoA synthetase long chain family member 1; Acyl-CoA synthetase 1; FACL 1; FACL 2; FACL1; FACL2; Fatty acid Coenzyme A ligase long chain 1; Fatty acid Coenzyme A ligase long chain 2; LACS 1; LACS 2; LACS; LACS1; LACS2; Lignoceroyl CoA synthase; Long chain acyl CoA synthetase 1; Long chain acyl CoA synthetase 2; Long chain fatty acid CoA ligase 1; Long chain fatty acid CoA ligase 2; Long chain fatty acid coenzyme A ligase 1; Long-chain acyl-CoA synthetase 1; Long-chain acyl-CoA synthetase 2; Long-chain fatty acid-CoA ligase 2; Long-chain-fatty-acid--CoA ligase 1; Palmitoyl CoA ligase 1; Palmitoyl CoA ligase 2; Palmitoyl-CoA ligase 1; Palmitoyl-CoA ligase 2; Paltimoyl CoA ligase 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P33121 ACSL1_HUMAN:

Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.

Sequence:
MQAHELFRYFRMPELVDFRQYVRTLPTNTLMGFGAFAALTTFWYATRPKPLKPPCDLSMQSVEVAGSGGARRSALLDSDEPLVYFYDDVTTLYEGFQRGIQVSNNGPCLGSRKPDQPYEWLSYKQVAELSECIGSALIQKGFKTAPDQFIGIFAQNRPEWVIIEQGCFAYSMVIVPLYDTLGNEAITYIVNKAELSLVFVDKPEKAKLLLEGVENKLIPGLKIIVVMDAYGSELVERGQRCGVEVTSMKAMEDLGRANRRKPKPPAPEDLAVICFTSGTTGNPKGAMVTHRNIVSDCSAFVKATENTVNPCPDDTLISFLPLAHMFERVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTVFPVVPRLLNRMFDRIFGQANTTLKRWLLDFASKRKEAELRSGIIRNNSLWDRLIFHKVQSSLGGRVRLMVTGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLTMPGDWTAGHVGAPMPCNLIKLVDVEEMNYMAAEGEGEVCVKGPNVFQGYLKDPAKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYMRSEPVAQVFVHGESLQAFLIAIVVPDVETLCSWAQKRGFEGSFEELCRNKDVKKAILEDMVRLGKDSGLKPFEQVKGITLHPELFSIDNGLLTPTMKAKRPELRNYFRSQIDDLYSTIKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Xenopus
100
Chicken
100
Dog
92
Bovine
91
Rabbit
44
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P33121 As Substrate

Site PTM Type Enzyme
S103 Phosphorylation
S122 Phosphorylation
Y188 Phosphorylation
K216 Ubiquitination
K222 Ubiquitination
S232 Phosphorylation
K356 Ubiquitination
K386 Acetylation
S394 Phosphorylation
K395 Acetylation
K395 Ubiquitination
S410 Phosphorylation
K419 Ubiquitination
S439 Phosphorylation
K532 Ubiquitination
Y567 Phosphorylation
Y577 Phosphorylation
S620 Phosphorylation
K643 Ubiquitination
S664 Phosphorylation
T671 Phosphorylation
Y693 Phosphorylation
T695 Phosphorylation

Research Backgrounds

Function:

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).

Subcellular Location:

Mitochondrion outer membrane>Single-pass type III membrane protein. Peroxisome membrane>Single-pass type III membrane protein. Microsome membrane>Single-pass type III membrane protein. Endoplasmic reticulum membrane>Single-pass type III membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.

Family&Domains:

Belongs to the ATP-dependent AMP-binding enzyme family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Metabolism > Lipid metabolism > Fatty acid biosynthesis.

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

References

1). MiR-103-3p promotes hepatic steatosis to aggravate nonalcoholic fatty liver disease by targeting of ACOX1. MOLECULAR BIOLOGY REPORTS (PubMed: 35606603) [IF=2.8]

Application: WB    Species: Mice    Sample: liver tissue

Fig. 3 Antagomir-103-3p alleviated the damage to mice with NAFLD. Mice with NAFLD were fed an HFD for 8 weeks, and Antagomir-NC or Antagomir-103-3p was used for tail vein injection once a week for 2 weeks. A MiR-103-3p expression in mouse liver tissues was examined by qRT-PCR. B Oil Red O staining detected lipid droplet accumulation in mouse liver tissues, and HE staining detected liver tissue lesions in mice. C The TG, ALT, AST and H2O2 contents in mouse serum were examined, while ROS generation and ATP content were examined in mouse tissues. D The protein and mRNA levels of ACOX1, FASN and ACSL1 were examined by western blotting and qRT-PCR, respectively. *P < 0.05 compared with the control group; #P < 0.05 compared with the NAFLD+Antagomir-NC group

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