LILRB2 Antibody | Affinity Biosciences

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Western blot analysis of LILRB2 using K562 whole cell lysates.

DF9604 at 1/100 staining human prostate cancer and para-carcinoma tissue by IHC-P.

Product:	LILRB2 Antibody
Catalog:	DF9604
Description:	Rabbit polyclonal antibody to LILRB2
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	60~80 kDa; 65kD(Calculated).
Uniprot:	Q8N423
RRID:	AB_2842800

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

LILRB2 Antibody detects endogenous levels of total LILRB2.

RRID:

AB_2842800
Cite Format: Affinity Biosciences Cat# DF9604, RRID:AB_2842800.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD85 antigen-like family member D; CD85d; CD85d antigen; ILT 4; ILT-4; ILT4; Immunoglobulin like transcript 4; Immunoglobulin-like transcript 4; Leukocyte immunoglobulin like receptor 2; Leukocyte immunoglobulin like receptor subfamily B member 2; Leukocyte immunoglobulin-like receptor 2; Leukocyte immunoglobulin-like receptor subfamily B member 2; Leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2; LILRA6; LILRB2; LIR 2; LIR-2; LIR2; LIRb; LIRB2_HUMAN; MIR 10; MIR-10; MIR10; Monocyte/macrophage immunoglobulin like receptor 10; Monocyte/macrophage immunoglobulin-like receptor 10;

Immunogens

Immunogen:

Uniprot:

Q8N423(LIRB2_HUMAN) >>Visit HPA database.

Expression:

Q8N423 LIRB2_HUMAN:

Expressed in monocytes and at lower levels in myeloid and plasmacytoid dendritic cells. Expressed in tolerogenic IL10-producing dendritic cells (PubMed:20448110). Expressed in myeloid-derived suppressor cells during pregnancy (PubMed:27859042). Detected at low levels in natural killer (NK) cells. Expressed in B cells.

Sequence:

Q8N423 LIRB2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTPIVTVLICLGLSLGPRTHVQTGTIPKPTLWAEPDSVITQGSPVTLSCQGSLEAQEYRLYREKKSASWITRIRPELVKNGQFHIPSITWEHTGRYGCQYYSRARWSELSDPLVLVMTGAYPKPTLSAQPSPVVTSGGRVTLQCESQVAFGGFILCKEGEEEHPQCLNSQPHARGSSRAIFSVGPVSPNRRWSHRCYGYDLNSPYVWSSPSDLLELLVPGVSKKPSLSVQPGPVVAPGESLTLQCVSDVGYDRFVLYKEGERDLRQLPGRQPQAGLSQANFTLGPVSRSYGGQYRCYGAHNLSSECSAPSDPLDILITGQIRGTPFISVQPGPTVASGENVTLLCQSWRQFHTFLLTKAGAADAPLRLRSIHEYPKYQAEFPMSPVTSAHAGTYRCYGSLNSDPYLLSHPSEPLELVVSGPSMGSSPPPTGPISTPAGPEDQPLTPTGSDPQSGLGRHLGVVIGILVAVVLLLLLLLLLFLILRHRRQGKHWTSTQRKADFQHPAGAVGPEPTDRGLQWRSSPAADAQEENLYAAVKDTQPEDGVEMDTRAAASEAPQDVTYAQLHSLTLRRKATEPPPSQEREPPAEPSIYATLAIH

PTMs - Q8N423 As Substrate

Q8N423

Site	PTM Type	Source
S66	Phosphorylation	Uniprot
T71	Phosphorylation	Uniprot
Y377	Phosphorylation	Uniprot
S384	Phosphorylation	Uniprot
Y533	Phosphorylation	Uniprot
T561	Phosphorylation	Uniprot
Y562	Phosphorylation	Uniprot
S567	Phosphorylation	Uniprot
Y592	Phosphorylation	Uniprot

Research Backgrounds

Q8N423_LIRB2_HUMAN

Function:

Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

PTMs:

Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Expressed in monocytes and at lower levels in myeloid and plasmacytoid dendritic cells. Expressed in tolerogenic IL10-producing dendritic cells. Expressed in myeloid-derived suppressor cells during pregnancy. Detected at low levels in natural killer (NK) cells. Expressed in B cells.

Subunit Structure:

Binds PTPN6 when phosphorylated. Binds FCGR1A. Interacts with peptide-bound HLA-G-B2M; this interaction is direct. Interacts with peptide-bound HLA-F-B2M; this interaction is direct.

Family&Domains:

Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

References

1). ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation. Theranostics, 2021 (PubMed: 33537094) [IF=12.4]

2). Novel macrophage-related gene prognostic index for glioblastoma associated with M2 macrophages and T cell dysfunction. Frontiers in Immunology, 2022 (PubMed: 36177003) [IF=7.3]

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LILRB2 Antibody - #DF9604