USP54 Antibody - #DF9595

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Product: USP54 Antibody
Catalog: DF9595
Description: Rabbit polyclonal antibody to USP54
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 187kDa; 187kD(Calculated).
Uniprot: Q70EL1
RRID: AB_2842791

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
USP54 Antibody detects endogenous levels of total USP54.
RRID:
AB_2842791
Cite Format: Affinity Biosciences Cat# DF9595, RRID:AB_2842791.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

USP54; bA137L10.3; bA137L10.4; C10orf29; FLJ37318; Inactive ubiquitin carboxyl-terminal hydrolase 54; nactive ubiquitin-specific peptidase 54;

Immunogens

Immunogen:
Uniprot:

Q70EL1(UBP54_HUMAN) >>Visit HPA database.

Gene(ID):
USP54(159195)
Expression:
Q70EL1 UBP54_HUMAN:

Weakly expressed in a few tissues.

Sequence:
MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Horse
91
Xenopus
91
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q70EL1 As Substrate

Site PTM Type Enzyme
Y7 Phosphorylation
R12 Methylation
S14 Phosphorylation
S23 Phosphorylation
S24 Phosphorylation
K92 Ubiquitination
K105 Ubiquitination
K203 Ubiquitination
K331 Ubiquitination
K336 Ubiquitination
T374 Phosphorylation
Y376 Phosphorylation
S378 Phosphorylation
S381 Phosphorylation
S388 Phosphorylation
S389 Phosphorylation
T442 Phosphorylation
T451 Phosphorylation
S452 Phosphorylation
S456 Phosphorylation
S481 Phosphorylation
R533 Methylation
R535 Methylation
K542 Methylation
Y572 Phosphorylation
T575 Phosphorylation
S582 Phosphorylation
K591 Ubiquitination
S603 Phosphorylation
K620 Ubiquitination
S632 Phosphorylation
Y635 Phosphorylation
R653 Methylation
S660 Phosphorylation
Y662 Phosphorylation
S671 Phosphorylation
S674 Phosphorylation
S682 Phosphorylation
S690 Phosphorylation
K738 Ubiquitination
K769 Ubiquitination
S854 Phosphorylation
S958 Phosphorylation
K983 Ubiquitination
S988 Phosphorylation
K998 Ubiquitination
K1029 Ubiquitination
S1036 Phosphorylation
S1056 Phosphorylation
Y1069 Phosphorylation
K1103 Ubiquitination
K1126 Ubiquitination
S1143 Phosphorylation
S1156 Phosphorylation
S1158 Phosphorylation
K1161 Ubiquitination
S1163 Phosphorylation
S1164 Phosphorylation
K1169 Ubiquitination
Y1233 Phosphorylation
K1236 Ubiquitination
K1246 Ubiquitination
K1275 Ubiquitination
S1352 Phosphorylation
S1362 Phosphorylation
S1408 Phosphorylation
S1420 Phosphorylation
S1436 Phosphorylation
K1444 Ubiquitination
K1521 Ubiquitination
Y1529 Phosphorylation
S1531 Phosphorylation
Y1568 Phosphorylation

Research Backgrounds

Function:

Has no peptidase activity.

Tissue Specificity:

Weakly expressed in a few tissues.

Family&Domains:

Belongs to the peptidase C19 family.

References

1). Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4. iScience, 2021 (PubMed: 36590171) [IF=5.8]

Application: WB    Species: Human    Sample: SGC7901 cells

Figure 5 USP54 combines with PLK4 and mediates its deubiquitination (A) Microarray analysis of mRNAs extracted from SGC7901-vector and SGC7901-CEP120 cells. (B) Results of the ubiquitination level of PLK4 in HEK293T cells, which were co-transfected with Flag-PLK4, HA-Ub, and one of the three DUBs. (C) WB results of USP54 and PLK4 expression in GC tissues. p value derived from Pearson correlation analysis. (D) WB results of PLK4 and USP54 after USP54 overexpression or knockdown. (E) CoIP analysis of the interaction between PLK4 and USP54 in SGC7901 and BGC823 cells. (F) HEK 293T cells were co-transfected with His-USP54 and Flag-PLK4 full length or Flag-PLK4 (1-740), Flag-PLK4 (1-270), and Flag-PLK4 (270-890) constructs and harvested for WB and IP analysis. (G) HEK 293T cells were co-transfected with His-USP54 and Flag-PLK4 wildtype or Flag-PLK4 lysine residue mutants and harvested for IP analysis. (H) Ubiquitination level of PLK4 after USP54 overexpression or knockdown examined by ubiquitination assay. (I) WB results of PLK4 in SGC7901 cells transfected with Lenti-CEP120 and siUSP54 and BGC823 cells transfected with Lenti-shCEP120 and USP54 plasmid.

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