Product: GTPBP5 Antibody
Catalog: DF9550
Description: Rabbit polyclonal antibody to GTPBP5
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Chicken
Mol.Wt.: 44 kDa; 44kD(Calculated).
Uniprot: Q9H4K7
RRID: AB_2842746

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Horse(83%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
GTPBP5 Antibody detects endogenous levels of total GTPBP5.
RRID:
AB_2842746
Cite Format: Affinity Biosciences Cat# DF9550, RRID:AB_2842746.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

dJ1005F21.2; FLJ10741; GTP binding protein 5 (putative); GTP binding protein 5; GTP-binding protein 5; GTPB5_HUMAN; GTPBP5; Mitochondrial ribosome-associated GTPase 2; ObgH1; Protein obg homolog 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAPARCFSARLRTVFQGVGHWALSTWAGLKPSRLLPQRASPRLLSVGRADLAKHQELPGKKLLSEKKLKRYFVDYRRVLVCGGNGGAGASCFHSEPRKEFGGPDGGDGGNGGHVILRVDQQVKSLSSVLSRYQGFSGEDGGSKNCFGRSGAVLYIRVPVGTLVKEGGRVVADLSCVGDEYIAALGGAGGKGNRFFLANNNRAPVTCTPGQPGQQRVLHLELKTVAHAGMVGFPNAGKSSLLRAISNARPAVASYPFTTLKPHVGIVHYEGHLQIAVADIPGIIRGAHQNRGLGSAFLRHIERCRFLLFVVDLSQPEPWTQVDDLKYELEMYEKGLSARPHAIVANKIDLPEAQANLSQLRDHLGQEVIVLSALTGENLEQLLLHLKVLYDAYAEAELGQGRQPLRW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
83
Horse
83
Chicken
83
Bovine
75
Sheep
75
Dog
75
Xenopus
67
Rabbit
50
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H4K7 As Substrate

Site PTM Type Enzyme
K123 Ubiquitination
S357 Phosphorylation
Y389 Phosphorylation

Research Backgrounds

Function:

Plays a role in the regulation of the mitochondrial ribosome assembly and of translational activity. Displays GTPase activity. Involved in the ribosome maturation process.

Subcellular Location:

Mitochondrion. Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Associates with the mitochondrial ribosome large subunit; the association occurs in a GTP-dependent manner.

Family&Domains:

Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.