RALGAPA1 Antibody - #DF9548

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Product: RALGAPA1 Antibody
Catalog: DF9548
Description: Rabbit polyclonal antibody to RALGAPA1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 230 kDa; 230kD(Calculated).
Uniprot: Q6GYQ0
RRID: AB_2842744

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(82%), Horse(100%), Sheep(82%), Rabbit(82%)
Clonality:
Polyclonal
Specificity:
RALGAPA1 Antibody detects endogenous levels of total RALGAPA1.
RRID:
AB_2842744
Cite Format: Affinity Biosciences Cat# DF9548, RRID:AB_2842744.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp566D133; DKFZp667F074; GAP related interacting partner to E12; GAP related interacting protein to E12; GARN L1; GARNL 1; GRIPE; GTPase activating RANGAP domain like 1; GTPase activating Rap/RanGAP domain like 1; KIAA0884; p240; Ral GTPase activating protein catalytic alpha subunit 1; Ral GTPase activating protein subunit alpha 1; Ral GTPase activating protein, alpha subunit 1 (catalytic); RalGAPalpha1; Tuberin like protein 1; TULIP 1; TULIP1;

Immunogens

Immunogen:
Uniprot:

Q6GYQ0(RGPA1_HUMAN) >>Visit HPA database.

Gene(ID):
RALGAPA1(253959)
Expression:
Q6GYQ0 RGPA1_HUMAN:

Widely expressed.

Sequence:
MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEHGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPILDIPQMRPKPHYVVIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREESKNDNADKTDRTTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVITSSDLPCIENVTDHDISMEEGEKREEENGTNTADHVRNSSWAKNGSYQGALHNASEEATEQNIRAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRSTRVRHFSQSEETGNEVFGALNEEQPLPRSSSTSDILEPFTVERAKVNKEDMSQKLPPLNSDIGGSSANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFGTDTGVTSSADVDSGSGHHQSAEEQEVASLTTLHIDSETSSLNQQAFSAEVATITGSESASPVHSPLGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPSLHPNIPDVAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPFHATGAESDKTEKSVLNCIYKVLHGCVYGAQCFSNPRYFPMSLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQHGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRSEENMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPPVSGLSEPTSFMLSLSHQEKPEEPPTSNECLEDITVKDGLSLQFKRFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGISLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAVEQDEPIPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
82
Sheep
82
Rabbit
82
Zebrafish
67
Chicken
60
Pig
0
Dog
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6GYQ0 As Substrate

Site PTM Type Enzyme
S13 Phosphorylation
T14 Phosphorylation
T20 Phosphorylation
K295 Ubiquitination
S342 Phosphorylation
S345 Phosphorylation
S349 Phosphorylation
S366 Phosphorylation
S368 Phosphorylation
S371 Phosphorylation
S379 Phosphorylation
S380 Phosphorylation
S381 Phosphorylation
S382 Phosphorylation
S385 Phosphorylation
S474 Phosphorylation
S497 Phosphorylation
S503 Phosphorylation
S598 Phosphorylation
T611 Phosphorylation
K689 Ubiquitination
S691 Phosphorylation
S711 Phosphorylation
K714 Acetylation
K714 Ubiquitination
S715 Phosphorylation
S721 Phosphorylation
S734 Phosphorylation
T736 Phosphorylation
S740 Phosphorylation
S748 Phosphorylation
T754 Phosphorylation
S773 Phosphorylation
S775 Phosphorylation
T778 Phosphorylation
S795 Phosphorylation
S796 Phosphorylation
S797 Phosphorylation
T798 Phosphorylation
S799 Phosphorylation
S831 Phosphorylation
S849 Phosphorylation
S853 Phosphorylation
S860 Phosphorylation
S861 Phosphorylation
S864 Phosphorylation
S958 Phosphorylation
S986 Phosphorylation
S990 Phosphorylation
S994 Phosphorylation
S1000 Phosphorylation
T1002 Phosphorylation
S1004 Phosphorylation
S1006 Phosphorylation
K1133 Methylation
S1324 Phosphorylation
T1368 Phosphorylation
T1370 Phosphorylation
S1478 Phosphorylation
S1554 Phosphorylation
T1565 Phosphorylation
S1569 Phosphorylation
S1575 Phosphorylation
S1585 Phosphorylation
S1590 Phosphorylation
S1593 Phosphorylation
Y1614 Phosphorylation
S1658 Phosphorylation
T1673 Phosphorylation
S1776 Phosphorylation
S1782 Phosphorylation
S1801 Phosphorylation
T1807 Phosphorylation
S1942 Phosphorylation
K1984 Ubiquitination

Research Backgrounds

Function:

Catalytic subunit of the heterodimeric RalGAP1 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocated to the nucleus, when associated with TCF3/E12.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Component of the heterodimeric RalGAP1 complex with RALGAPB. Heterodimerization is required for activity. Interacts with the HLH region of TCF3/isoform E12 (By similarity).

Restrictive clause

 

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