GPSM2 Antibody - #DF9540
| Product: | GPSM2 Antibody |
| Catalog: | DF9540 |
| Description: | Rabbit polyclonal antibody to GPSM2 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Dog, Chicken |
| Mol.Wt.: | 77 kDa; 77kD(Calculated). |
| Uniprot: | P81274 |
| RRID: | AB_2842736 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9540, RRID:AB_2842736.
Fold/Unfold
DFNB82; G protein signalling modulator 2 (AGS3 like C. elegans); G protein signalling modulator 2; G-protein-signaling modulator 2; Gpsm2; GPSM2_HUMAN; HGNC:29501; LGN; LGN protein; Mosaic protein LGN; Pins;
Immunogens
- P81274 GPSM2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEENLISMREDHSFHVRYRMEASCLELALEGERLCKSGDCRAGVSFFEAAVQVGTEDLKTLSAIYSQLGNAYFYLHDYAKALEYHHHDLTLARTIGDQLGEAKASGNLGNTLKVLGNFDEAIVCCQRHLDISRELNDKVGEARALYNLGNVYHAKGKSFGCPGPQDVGEFPEEVRDALQAAVDFYEENLSLVTALGDRAAQGRAFGNLGNTHYLLGNFRDAVIAHEQRLLIAKEFGDKAAERRAYSNLGNAYIFLGEFETASEYYKKTLLLARQLKDRAVEAQSCYSLGNTYTLLQDYEKAIDYHLKHLAIAQELNDRIGEGRACWSLGNAYTALGNHDQAMHFAEKHLEISREVGDKSGELTARLNLSDLQMVLGLSYSTNNSIMSENTEIDSSLNGVRPKLGRRHSMENMELMKLTPEKVQNWNSEILAKQKPLIAKPSAKLLFVNRLKGKKYKTNSSTKVLQDASNSIDHRIPNSQRKISADTIGDEGFFDLLSRFQSNRMDDQRCCLQEKNCHTASTTTSSTPPKMMLKTSSVPVVSPNTDEFLDLLASSQSRRLDDQRASFSNLPGLRLTQNSQSVLSHLMTNDNKEADEDFFDILVKCQGSRLDDQRCAPPPATTKGPTVPDEDFFSLILRSQGKRMDEQRVLLQRDQNRDTDFGLKDFLQNNALLEFKNSGKKSADH
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P81274 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K103 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| Y146 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| Y252 | Phosphorylation | Uniprot | |
| Y264 | Phosphorylation | Uniprot | |
| Y265 | Phosphorylation | Uniprot | |
| S408 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T418 | Phosphorylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| T457 | Phosphorylation | Q96KB5 (PBK) | Uniprot |
| S459 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| T461 | Phosphorylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| T486 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| T518 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| T521 | Phosphorylation | Uniprot | |
| T522 | Phosphorylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| T526 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | Uniprot | |
| S541 | Phosphorylation | Uniprot | |
| T544 | Phosphorylation | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| S565 | Phosphorylation | Uniprot | |
| S567 | Phosphorylation | Uniprot | |
| S607 | Phosphorylation | Uniprot | |
| S633 | Phosphorylation | Uniprot | |
| S638 | Phosphorylation | Uniprot | |
| T658 | Phosphorylation | Uniprot | |
| K675 | Ubiquitination | Uniprot | |
| S677 | Phosphorylation | Uniprot | |
| S681 | Phosphorylation | Uniprot |
Research Backgrounds
Plays an important role in mitotic spindle pole organization via its interaction with NUMA1. Required for cortical dynein-dynactin complex recruitment during metaphase. Plays a role in metaphase spindle orientation. Plays also an important role in asymmetric cell divisions. Has guanine nucleotide dissociation inhibitor (GDI) activity towards G(i) alpha proteins, such as GNAI1 and GNAI3, and thereby regulates their activity (By similarity).
Cytoplasm. Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton>Spindle pole. Lateral cell membrane.
Note: Localizes in the cytoplasm during interphase and at cell cortex during metaphase (PubMed:11781568, PubMed:15632202, PubMed:22074847). Colocalizes with NUMA1 to mitotic spindle poles (PubMed:11781568, PubMed:21816348). Localized at the central and lateral cell cortex regions in a RanGTP-dependent manner (PubMed:22327364). In horizontally retinal progenitor dividing cells, localized to the lateral cortical region. In vertically retinal progenitor dividing cells, localized at the polar cortical region (By similarity).
Ubiquitously expressed.
Interacts with the dynein-dynactin complex; this interaction is inhibited in a PLK1-dependent manner. Part of a spindle orientation complex at least composed of GNAI1, GPSM2 and NUMA1. Interacts with LLGL2. Interacts (via TPR repeat region) with INSC/inscuteable. Interacts (via TPR repeat region) with NUMA1 (via C-terminus); this interaction is direct, inhibited in a PLK1-dependent manner, prevents the binding of NUMA1 with SPAG5 and promotes spindle pole organization. INSC and NUMA1 compete for the same binding site, but INSC has higher affinity and can displace NUMA1 (in vitro). Interacts with GNAI2. Interacts (via GoLoco domains) with the GDP-bound form of GNAI1 and GNAI3; has much lower affinity for the GTP-bound form. Interaction with GDP-bound GNAI3 strongly enhances the affinity for NUMA1 (By similarity). Interacts (via TPR repeat region) with FRMPD1. INSC and FRMPD1 compete for the same binding site, but INSC has higher affinity and can displace FRMPD1 (in vitro) (By similarity). Interacts (via TPR repeat region) with FRMPD4. Identified in a complex with INSC and F2RL2/Par3.
Belongs to the GPSM family.
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