GORASP1 Antibody - #DF9536
| Product: | GORASP1 Antibody |
| Catalog: | DF9536 |
| Description: | Rabbit polyclonal antibody to GORASP1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 46 kDa; 46kD(Calculated). |
| Uniprot: | Q9BQQ3 |
| RRID: | AB_2842732 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9536, RRID:AB_2842732.
Fold/Unfold
FLJ23443; Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; Golgi reassembly and stacking protein 1; Golgi reassembly and stacking protein 65 kDa; Golgi reassembly and stacking protein, 65-kD; Golgi reassembly stacking protein 1 65kDa; Golgi reassembly stacking protein 1; Golgi reassembly stacking protein of 65 kDa; Golgi reassembly-stacking protein 1; Golgi reassembly-stacking protein of 65 kDa; GOLPH 5; GOLPH5; Gorasp 1; GORASP1; GORS1_HUMAN; GRASP 65; GRASP65; MGC118894; MGC118897; P65;
Immunogens
- Q9BQQ3 GORS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGLGVSAEQPAGGAEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLKANVEKPVKLEVFNMKTMRVREVEVVPSNMWGGQGLLGASVRFCSFRRASEQVWHVLDVEPSSPAALAGLRPYTDYVVGSDQILQESEDFFTLIESHEGKPLKLMVYNSKSDSCREVTVTPNAAWGGEGSLGCGIGYGYLHRIPTQPPSYHKKPPGTPPPSALPLGAPPPDALPPGPTPEDSPSLETGSRQSDYMEALLQAPGSSMEDPLPGPGSPSHSAPDPDGLPHFMETPLQPPPPVQRVMDPGFLDVSGISLLDNSNASVWPSLPSSTELTTTAVSTSGPEDICSSSSSHERGGEATWSGSEFEVSFLDSPGAQAQADHLPQLTLPDSLTSAASPEDGLSAELLEAQAEEEPASTEGLDTGTEAEGLDSQAQISTTE
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BQQ3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K50 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | P53350 (PLK1) | Uniprot |
| T204 | Phosphorylation | Uniprot | |
| T216 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| T237 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| Y253 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | P45984 (MAPK9) , P28482 (MAPK1) | Uniprot |
| S278 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot |
Research Backgrounds
Plays an important role in assembly and membrane stacking of the Golgi cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes. Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane.
Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher-order oligomers, leading to cisternal unstacking.
Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis.
Myristoylated.
Golgi apparatus>cis-Golgi network membrane>Peripheral membrane protein>Cytoplasmic side. Endoplasmic reticulum-Golgi intermediate compartment membrane.
Homodimer. Forms higher-order oligomers under interphase but not mitotic conditions. Dimers of the protein on one membrane might be able to interact with dimers on another and so stack cisternae. Interacts with the C-terminus of GOLGA2/GM130 under both mitotic and non-mitotic conditions. The interaction is critical for the correct targeting of both proteins to the cis-Golgi. Interacts with TMED2 and TMED3 (By similarity).
Belongs to the GORASP family.
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