QSER1 Antibody - #DF9533
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Product: | QSER1 Antibody |
Catalog: | DF9533 |
Description: | Rabbit polyclonal antibody to QSER1 |
Application: | WB IHC |
Reactivity: | Human, Mouse |
Prediction: | Pig, Bovine, Sheep, Dog |
Mol.Wt.: | 190 kDa; 190kD(Calculated). |
Uniprot: | Q2KHR3 |
RRID: | AB_2842729 |
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
QSER1 Antibody detects endogenous levels of total QSER1.
RRID:
AB_2842729
Cite Format: Affinity Biosciences Cat# DF9533, RRID:AB_2842729.
Cite Format: Affinity Biosciences Cat# DF9533, RRID:AB_2842729.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
FLJ21924; Glutamine and serine rich 1; Glutamine and serine-rich protein 1;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q2KHR3 QSER1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNFLSTAESRTAQAAASGTTLLPQFRAPSWQTGMHSSAATELFATGPLPSTGTLPPSLSAYQHPTTFSNRNFATTSPLVLQDSTFNTTSNGILSHHDPLLQIKTSQGTVPTALAFERLGSSVLSNSIPPQSSTYRSAQESAPHLLQPQFSLLPSALGGSQQTPQAYSSTLFTSSTASIERALLRECSVIKHHQRPSGTQSIQAQLTGSQHSLHSYLSNSSVVNFQETTRQSSLSCSPIGDSTQVSNGGLQQKTSQVSVELAQSYSSAIPSSGYPPSTTKIKSCSTEQPLTSTKTPKPQSIIPPVQTLSYSKPLHNQSSVISGQAQIYSTAQLPSLLSVSQSQNYGLVQPHNVPSIVHSQVYRSSKVEKLPPLYKTLTFSGSSQTVTPENQTLNYSSNQQEVLSSVTNENYPAQTRDLSSVSQSQSYSSGHSQGLSPVSQTQVSYSSQSQVLSVVSLSESYASGESLTLTAPSLSYSSASRAQNLPDSSPTQNYISMHSSQNVQTQESSSPQSQKFLPAVQSSSFASSTHCQTLQNNITSPDPKSYAERKLDSDVYPSSKQEDGFPMQELQVLQPQASLESSTQRLSDGEINAQESTYKVSKADDRYSQSVIRSNSRLEDQVIGVALQASKKEESVVGSVTQLNQQIGQVNNAATLDLKNSTNLIQTPQIRLNTKDLKQQHPLILKVHESKVQEQHDQIINASSQIQIPNHALGHGHQASLPNTQVLLDSACDLQILQQSILQAGLGQVKASLQAQRVQSPQQIVHPFLQMEGHVIQSNGDHSQQQLHPQNSEVMKMDLSESSKPLQQHLTTKGHFSETNQHDSKNQFVSLGSMCFPEAVLLSDERNILSNVDDILAATAAACGVTPTDFSKSTSNETMQAVEDGDSKSHFQQSLDVRHVTSDFNSMTATVGKPQNINDTSLNGNQVTVNLSPVPALQSKMTLDQQHIETPGQNIPTKVTSAVVGPSHEVQEQSSGPFKKQSATNLESEEDSEAPVDSTLNNNRNQEFVSSSRSISGENATSESEFTLGGDDSGVSMNPARSALALLAMAQSGDAVSVKIEEENQDLMHFNLQKKRAKGKGQVKEEDNSNQKQLKRPAQGKRQNPRGTDIYLPYTPPSSESCHDGYQHQEKMRQKIKEVEEKQPEVKTGFIASFLDFLKSGPKQQFSTLAVRMPNRTRRPGTQMVRTFCPPPLPKPSSTTPTPLVSETGGNSPSDKVDNELKNLEHLSSFSSDEDDPGYSQDAYKSVSTPLTTLDATSDKKKKTEALQVATTSPTANTTGTATTSSTTVGAVKQEPLHSTSYAVNILENISSSESSKPIELDGLPSDQFAKGQDTVAIEGFTDEEDTESGGEGQYRERDEFVVKIEDIETFKEALKTGKEPPAIWKVQKALLQKFVPEIRDGQREFAATNSYLGYFGDAKSKYKRIYVKFIENANKKEYVRVCSKKPRNKPSQTIRTVQAKPSSSSKTSDPLASKTTTTKAPSVKPKVKQPKVKAEPPPKKRKKWKEEFSSSQSDSSPEIHTSSSDDEEFEPPAPFVTRFLNTRAMKETFKSYMELLVSIALDPDTMQALEKSNDELLLPHMKKIDGMLNDNRKRLLLNLHLDQSFKNALESFPELTIITRDSKAKSGGTAISKIKMNGKAYNKKTLRTSKTTTKSAQEFAVDPEKIQLYSLYHSLHHYKYHVYLICKDEISSVQKKNEDLGQEEIVQLCMKNVKWVEDLFEKFGELLNHVQQKCS
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
92
Rabbit
75
Chicken
67
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q2KHR3 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
R26 | Methylation | Uniprot | |
T104 | O-Glycosylation | Uniprot | |
S105 | O-Glycosylation | Uniprot | |
S236 | Phosphorylation | Uniprot | |
T242 | Phosphorylation | Uniprot | |
K281 | Methylation | Uniprot | |
K281 | Sumoylation | Uniprot | |
K281 | Ubiquitination | Uniprot | |
S282 | Phosphorylation | Uniprot | |
K368 | Ubiquitination | Uniprot | |
S488 | Phosphorylation | Uniprot | |
T490 | Phosphorylation | Uniprot | |
Y493 | Phosphorylation | Uniprot | |
S512 | Phosphorylation | Uniprot | |
K549 | Sumoylation | Uniprot | |
K549 | Ubiquitination | Uniprot | |
S552 | Phosphorylation | Uniprot | |
S580 | Phosphorylation | Uniprot | |
S586 | Phosphorylation | Uniprot | |
K598 | Ubiquitination | Uniprot | |
Y606 | Phosphorylation | Uniprot | |
S607 | Phosphorylation | Uniprot | |
S613 | Phosphorylation | Uniprot | |
S615 | Phosphorylation | Uniprot | |
S634 | Phosphorylation | Uniprot | |
T666 | Phosphorylation | Uniprot | |
K674 | Ubiquitination | Uniprot | |
K677 | Sumoylation | Uniprot | |
K677 | Ubiquitination | Uniprot | |
K685 | Ubiquitination | Uniprot | |
K803 | Acetylation | Uniprot | |
K803 | Sumoylation | Uniprot | |
K803 | Ubiquitination | Uniprot | |
K812 | Ubiquitination | Uniprot | |
K824 | Ubiquitination | Uniprot | |
S832 | Phosphorylation | Uniprot | |
S842 | Phosphorylation | Uniprot | |
T865 | Phosphorylation | Uniprot | |
S886 | Phosphorylation | Uniprot | |
K887 | Sumoylation | Uniprot | |
S931 | Phosphorylation | Uniprot | |
T949 | Phosphorylation | Uniprot | |
S966 | Phosphorylation | Uniprot | |
S974 | Phosphorylation | Uniprot | |
K978 | Acetylation | Uniprot | |
T983 | Phosphorylation | Uniprot | |
S987 | Phosphorylation | Uniprot | |
S1010 | Phosphorylation | Uniprot | |
S1011 | Phosphorylation | Uniprot | |
S1013 | Phosphorylation | Uniprot | |
S1015 | Phosphorylation | Uniprot | |
S1032 | Phosphorylation | Uniprot | |
S1035 | Phosphorylation | Uniprot | |
S1041 | Phosphorylation | Uniprot | |
K1058 | Sumoylation | Uniprot | |
K1083 | Sumoylation | Uniprot | |
Y1113 | Phosphorylation | Uniprot | |
T1114 | Phosphorylation | Uniprot | |
K1141 | Sumoylation | Uniprot | |
K1162 | Ubiquitination | Uniprot | |
R1171 | Methylation | Uniprot | |
R1178 | Methylation | Uniprot | |
R1185 | Methylation | Uniprot | |
T1199 | Phosphorylation | Uniprot | |
T1207 | Phosphorylation | Uniprot | |
S1211 | Phosphorylation | Uniprot | |
S1227 | Phosphorylation | Uniprot | |
S1228 | Phosphorylation | Uniprot | |
S1230 | Phosphorylation | Uniprot | |
S1231 | Phosphorylation | Uniprot | |
S1239 | Phosphorylation | Uniprot | |
T1248 | Phosphorylation | Uniprot | |
T1256 | Phosphorylation | Uniprot | |
S1257 | Phosphorylation | Uniprot | |
T1271 | Phosphorylation | Uniprot | |
T1277 | Phosphorylation | Uniprot | |
K1292 | Sumoylation | Uniprot | |
K1316 | Sumoylation | Uniprot | |
T1341 | Phosphorylation | Uniprot | |
T1346 | Phosphorylation | Uniprot | |
S1348 | Phosphorylation | Uniprot | |
Y1354 | Phosphorylation | Uniprot | |
K1388 | Sumoylation | Uniprot | |
K1388 | Ubiquitination | Uniprot | |
K1393 | Ubiquitination | Uniprot | |
Y1411 | Phosphorylation | Uniprot | |
K1493 | Sumoylation | Uniprot | |
K1550 | Methylation | Uniprot | |
S1551 | Phosphorylation | Uniprot | |
Y1552 | Phosphorylation | Uniprot | |
S1558 | Phosphorylation | Uniprot | |
K1583 | Ubiquitination | Uniprot | |
S1626 | Phosphorylation | Uniprot | |
T1629 | Phosphorylation | Uniprot | |
K1633 | Sumoylation | Uniprot | |
K1654 | Ubiquitination | Uniprot | |
K1714 | Ubiquitination | Uniprot | |
K1733 | Ubiquitination | Uniprot |
Research Backgrounds
References
1). Enhanced Activity against Multi-drug Resistant Bacteria through Coapplication of an Analogue of Tachyplesin I and an Inhibitor of the QseC/B Signaling Pathway. Journal of Medicinal Chemistry, 2020
(PubMed: 32003561)
[IF=7.3]
Application: WB Species: Sample:
Figure S8. Western blotting analysis of QseB expression in L. enzymogenes YC36
strain after TPAD was added, untreated L. enzymogenes YC36 strain (-) and L.
enzymogenes YC36 strain with 2 μg/ml TPAD. Western blot results showed that after
TPAD treated , qseB expression was up-regulated, which was consistent with qPCR
and transcriptome result.
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