Cytokeratin 8 Antibody - #AF0196

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Product: Cytokeratin 8 Antibody
Catalog: AF0196
Description: Rabbit polyclonal antibody to Cytokeratin 8
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 55kDa; 54kD(Calculated).
Uniprot: P05787
RRID: AB_2833389

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Cytokeratin 8 Antibody detects endogenous levels of total Cytokeratin 8.
RRID:
AB_2833389
Cite Format: Affinity Biosciences Cat# AF0196, RRID:AB_2833389.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CARD2; CK 8; CK-8; CK8; CYK8; CYKER; Cytokeratin endo A; Cytokeratin-8; DreK8; EndoA; K2C8; K2C8_HUMAN; K8; Keratin 8; Keratin type II cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; KO; Krt 2.8; KRT8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8;

Immunogens

Immunogen:
Uniprot:

P05787(K2C8_HUMAN) >>Visit HPA database.

Gene(ID):
KRT8(3856)
Expression:
P05787 K2C8_HUMAN:

Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

Description:
K8 a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization.
Sequence:
MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK

PTMs - P05787 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
T6 Phosphorylation Q96GD4 (AURKB)
K8 Acetylation
K8 Methylation
K8 Ubiquitination
S9 Phosphorylation P17612 (PRKACA)
Y10 Phosphorylation
K11 Acetylation
K11 Methylation
K11 Sumoylation
K11 Ubiquitination
S13 O-Glycosylation
S13 Phosphorylation P17612 (PRKACA)
T14 Phosphorylation
S15 O-Glycosylation
S15 Phosphorylation
R18 Methylation
S21 Phosphorylation
S22 Phosphorylation
R23 Methylation
S24 Phosphorylation P17612 (PRKACA)
Y25 Phosphorylation
T26 Phosphorylation
S27 Phosphorylation
S31 Phosphorylation
R32 Methylation
S34 Phosphorylation P17612 (PRKACA)
S35 Phosphorylation
S36 Phosphorylation
S37 Phosphorylation P17612 (PRKACA)
S39 Phosphorylation
R40 Methylation
S43 Phosphorylation P17612 (PRKACA)
S44 Phosphorylation
R47 Methylation
Y54 Phosphorylation
S58 Phosphorylation
T64 Phosphorylation
T67 Phosphorylation
S71 Phosphorylation
S74 Phosphorylation P36507 (MAP2K2) , O15264 (MAPK13) , Q05655 (PRKCD) , P53778 (MAPK12) , Q16539 (MAPK14) , Q15759 (MAPK11) , Q02750 (MAP2K1) , P45983 (MAPK8)
T89 Phosphorylation
K92 Ubiquitination
K96 Ubiquitination
T97 Phosphorylation
K101 Acetylation
K101 Methylation
K101 Sumoylation
K101 Ubiquitination
S104 Phosphorylation
K108 Acetylation
K108 Ubiquitination
K117 Acetylation
K117 Ubiquitination
K122 Acetylation
K122 Methylation
K122 Ubiquitination
S124 Phosphorylation
K130 Acetylation
K130 Sumoylation
K130 Ubiquitination
T131 Phosphorylation
S134 Phosphorylation
S142 Phosphorylation
Y143 Phosphorylation
K158 Acetylation
K158 Ubiquitination
K160 Ubiquitination
K176 Ubiquitination
K178 Methylation
K178 Ubiquitination
Y179 Phosphorylation
K185 Methylation
K185 Ubiquitination
T187 Phosphorylation
K197 Sumoylation
K197 Ubiquitination
K198 Ubiquitination
Y204 Phosphorylation
K207 Acetylation
K207 Ubiquitination
S212 Phosphorylation
T218 Phosphorylation
Y228 Phosphorylation
S240 Phosphorylation
T242 Phosphorylation
S243 Phosphorylation
S247 Phosphorylation
S251 Phosphorylation
S253 Phosphorylation
S258 Phosphorylation
K264 Ubiquitination
Y267 Phosphorylation
S274 Phosphorylation
S280 Phosphorylation
Y282 Phosphorylation
K285 Acetylation
K285 Methylation
K285 Sumoylation
K285 Ubiquitination
Y286 Phosphorylation
S291 Phosphorylation
K295 Acetylation
K295 Ubiquitination
T303 Phosphorylation
K304 Sumoylation
K304 Ubiquitination
T305 Phosphorylation
S308 Phosphorylation
S315 Phosphorylation
K325 Acetylation
K325 Sumoylation
K325 Ubiquitination
S330 Phosphorylation
K347 Acetylation
K347 Ubiquitination
K352 Acetylation
K352 Ubiquitination
S354 Phosphorylation
K364 Sumoylation
Y374 Phosphorylation
K393 Acetylation
K393 Sumoylation
K393 Ubiquitination
S400 Phosphorylation
S404 Phosphorylation
S410 Phosphorylation
T413 Phosphorylation
T415 Phosphorylation
T416 Phosphorylation
S417 Phosphorylation P17612 (PRKACA)
Y419 Phosphorylation
S424 Phosphorylation
S425 Phosphorylation
Y427 Phosphorylation
T431 Phosphorylation P28482 (MAPK1)
S432 Phosphorylation P27361 (MAPK3) , P45983 (MAPK8) , P06493 (CDK1)
S436 Phosphorylation
Y437 Phosphorylation
S438 Phosphorylation
S441 Phosphorylation
S442 Phosphorylation
S445 Phosphorylation
S449 Phosphorylation
S450 Phosphorylation
S451 Phosphorylation
S453 Phosphorylation
T455 Phosphorylation
S456 Phosphorylation
S457 Phosphorylation
S458 Phosphorylation
K464 Acetylation
K465 Acetylation
K465 Ubiquitination
T468 Phosphorylation
K472 Acetylation
K472 Sumoylation
K472 Ubiquitination
S475 O-Glycosylation
S475 Phosphorylation
S477 Phosphorylation
S478 Phosphorylation
K483 Acetylation

Research Backgrounds

Function:

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

PTMs:

Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.

O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Subcellular Location:

Cytoplasm. Nucleus>Nucleoplasm. Nucleus matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1. Interacts with GPER1. Interacts with EPPK1 (By similarity).

(Microbial infection) Interacts with hepatitis C virus/HCV core protein.

Family&Domains:

Belongs to the intermediate filament family.

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