GPATCH8 Antibody - #DF9522

(1)   (4)  
Product: GPATCH8 Antibody
Catalog: DF9522
Description: Rabbit polyclonal antibody to GPATCH8
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 164 kDa; 164kD(Calculated).
Uniprot: Q9UKJ3
RRID: AB_2842718

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(91%), Sheep(91%), Rabbit(82%), Dog(91%)
Clonality:
Polyclonal
Specificity:
GPATCH8 Antibody detects endogenous levels of total GPATCH8.
RRID:
AB_2842718
Cite Format: Affinity Biosciences Cat# DF9522, RRID:AB_2842718.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

G patch domain containing 8; G patch domain-containing protein 8; GPATC8; Gpatch8; GPTC8_HUMAN; KIAA0553;

Immunogens

Immunogen:
Uniprot:

Q9UKJ3(GPTC8_HUMAN) >>Visit HPA database.

Gene(ID):
GPATCH8(23131)
Sequence:
MADRFSRFNEDRDFQGNHFDQYEEGHLEIEQASLDKPIESDNIGHRLLQKHGWKLGQGLGKSLQGRTDPIPIVVKYDVMGMGRMEMELDYAEDATERRRVLEVEKEDTEELRQKYKDYVDKEKAIAKALEDLRANFYCELCDKQYQKHQEFDNHINSYDHAHKQRLKDLKQREFARNVSSRSRKDEKKQEKALRRLHELAEQRKQAECAPGSGPMFKPTTVAVDEEGGEDDKDESATNSGTGATASCGLGSEFSTDKGGPFTAVQITNTTGLAQAPGLASQGISFGIKNNLGTPLQKLGVSFSFAKKAPVKLESIASVFKDHAEEGTSEDGTKPDEKSSDQGLQKVGDSDGSSNLDGKKEDEDPQDGGSLASTLSKLKRMKREEGAGATEPEYYHYIPPAHCKVKPNFPFLLFMRASEQMDGDNTTHPKNAPESKKGSSPKPKSCIKAAASQGAEKTVSEVSEQPKETSMTEPSEPGSKAEAKKALGGDVSDQSLESHSQKVSETQMCESNSSKETSLATPAGKESQEGPKHPTGPFFPVLSKDESTALQWPSELLIFTKAEPSISYSCNPLYFDFKLSRNKDARTKGTEKPKDIGSSSKDHLQGLDPGEPNKSKEVGGEKIVRSSGGRMDAPASGSACSGLNKQEPGGSHGSETEDTGRSLPSKKERSGKSHRHKKKKKHKKSSKHKRKHKADTEEKSSKAESGEKSKKRKKRKRKKNKSSAPADSERGPKPEPPGSGSPAPPRRRRRAQDDSQRRSLPAEEGSSGKKDEGGGGSSSQDHGGRKHKGELPPSSCQRRAGTKRSSRSSHRSQPSSGDEDSDDASSHRLHQKSPSQYSEEEEEEDSGSEHSRSRSRSGRRHSSHRSSRRSYSSSSDASSDQSCYSRQRSYSDDSYSDYSDRSRRHSKRSHDSDDSDYASSKHRSKRHKYSSSDDDYSLSCSQSRSRSRSHTRERSRSRGRSRSSSCSRSRSKRRSRSTTAHSWQRSRSYSRDRSRSTRSPSQRSGSRKRSWGHESPEERHSGRRDFIRSKIYRSQSPHYFRSGRGEGPGKKDDGRGDDSKATGPPSQNSNIGTGRGSEGDCSPEDKNSVTAKLLLEKIQSRKVERKPSVSEEVQATPNKAGPKLKDPPQGYFGPKLPPSLGNKPVLPLIGKLPATRKPNKKCEESGLERGEEQEQSETEEGPPGSSDALFGHQFPSEETTGPLLDPPPEESKSGEATADHPVAPLGTPAHSDCYPGDPTISHNYLPDPSDGDTLESLDSSSQPGPVESSLLPIAPDLEHFPSYAPPSGDPSIESTDGAEDASLAPLESQPITFTPEEMEKYSKLQQAAQQHIQQQLLAKQVKAFPASAALAPATPALQPIHIQQPATASATSITTVQHAILQHHAAAAAAAIGIHPHPHPQPLAQVHHIPQPHLTPISLSHLTHSIIPGHPATFLASHPIHIIPASAIHPGPFTFHPVPHAALYPTLLAPRPAAAAATALHLHPLLHPIFSGQDLQHPPSHGT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
91
Sheep
91
Dog
91
Rabbit
82
Chicken
73
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UKJ3 As Substrate

Site PTM Type Enzyme
K50 Ubiquitination
K54 Ubiquitination
S62 Phosphorylation
Y76 Phosphorylation
Y118 Phosphorylation
Y137 Phosphorylation
K188 Methylation
T293 Phosphorylation
S301 Phosphorylation
S303 Phosphorylation
T327 Phosphorylation
S328 Phosphorylation
S349 Phosphorylation
K376 Ubiquitination
Y394 Phosphorylation
S444 Phosphorylation
S451 Phosphorylation
K456 Acetylation
T457 Phosphorylation
S459 Phosphorylation
S469 Phosphorylation
S491 Phosphorylation
S499 Phosphorylation
S510 Phosphorylation
S517 Phosphorylation
T520 Phosphorylation
S526 Phosphorylation
S542 Phosphorylation
Y573 Phosphorylation
S635 Phosphorylation
S637 Phosphorylation
S640 Phosphorylation
S650 Phosphorylation
S653 Phosphorylation
T655 Phosphorylation
S738 Phosphorylation
S740 Phosphorylation
S758 Phosphorylation
S766 Phosphorylation
S815 Phosphorylation
S832 Phosphorylation
S837 Phosphorylation
S845 Phosphorylation
S847 Phosphorylation
S861 Phosphorylation
S862 Phosphorylation
S871 Phosphorylation
S874 Phosphorylation
S878 Phosphorylation
C882 S-Nitrosylation
Y889 Phosphorylation
S893 Phosphorylation
Y894 Phosphorylation
S898 Phosphorylation
S908 Phosphorylation
S911 Phosphorylation
S914 Phosphorylation
Y916 Phosphorylation
S923 Phosphorylation
S931 Phosphorylation
S940 Phosphorylation
S942 Phosphorylation
S944 Phosphorylation
S954 Phosphorylation
S960 Phosphorylation
S974 Phosphorylation
S976 Phosphorylation
T977 Phosphorylation
T978 Phosphorylation
S981 Phosphorylation
S985 Phosphorylation
S987 Phosphorylation
S1009 Phosphorylation
S1014 Phosphorylation
Y1031 Phosphorylation
S1033 Phosphorylation
S1035 Phosphorylation
Y1038 Phosphorylation
S1058 Phosphorylation
S1065 Phosphorylation
T1072 Phosphorylation
S1076 Phosphorylation
S1081 Phosphorylation
K1096 Acetylation
S1107 Phosphorylation
S1109 Phosphorylation
T1115 Phosphorylation
Y1130 Phosphorylation
K1142 Acetylation
K1150 Acetylation
S1260 Phosphorylation
K1322 Acetylation
K1322 Ubiquitination
K1338 Ubiquitination
K1341 Ubiquitination

Research Backgrounds

References

1). Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis. PLOS Neglected Tropical Diseases, 2017 (PubMed: 36206314) [IF=3.8]

Application: WB    Species: Mouse    Sample:

Fig 1. The dynamic changes of mouse proteins in serum-derived exosomes in response to E. multilocularis infection. (A) and (B) Transmission electron microscopy of the enriched extracellular vesicles (EVs) isolated from the sera of E. multilocularis-infected (A) and uninfected mice (B). (C) The diameter distribution of the purified EVs. (D) Western blotting analysis of mammalian EV-marker CD63 in the purified EVs. (E) Heatmap of differentially abundance proteins between the serum exosomes from E. multilocularis-infected and uninfected mice. (F) Western blotting analysis the abundance of TGF-β1, GPATC8, LRP1, and AMY1 during E. multilocularis infection. https://doi.org/10.1371/journal.pntd.0010814.g001

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.