KHSRP Antibody - #DF9514

(3)  
Product: KHSRP Antibody
Catalog: DF9514
Description: Rabbit polyclonal antibody to KHSRP
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Chicken, Xenopus
Mol.Wt.: 73 kDa; 73kD(Calculated).
Uniprot: Q92945
RRID: AB_2842710

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
KHSRP Antibody detects endogenous levels of total KHSRP.
RRID:
AB_2842710
Cite Format: Affinity Biosciences Cat# DF9514, RRID:AB_2842710.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Far upstream element-binding protein 2; FBP2; FUBP2; FUBP2_HUMAN; FUSE binding protein 2; FUSE-binding protein 2; KH type splicing regulatory protein (FUSE binding protein 2); KH type splicing regulatory protein; KH type-splicing regulatory protein; KHSRP; KSRP; p75;

Immunogens

Immunogen:
Uniprot:

Q92945(FUBP2_HUMAN) >>Visit HPA database.

Gene(ID):
KHSRP(8570)
Expression:
Q92945 FUBP2_HUMAN:

Detected in neural and non-neural cell lines.

Sequence:
MSDYSTGGPPPGPPPPAGGGGGAGGAGGGPPPGPPGAGDRGGGGPGGGGPGGGSAGGPSQPPGGGGPGIRKDAFADAVQRARQIAAKIGGDAATTVNNSTPDFGFGGQKRQLEDGDQPESKKLASQGDSISSQLGPIHPPPRTSMTEEYRVPDGMVGLIIGRGGEQINKIQQDSGCKVQISPDSGGLPERSVSLTGAPESVQKAKMMLDDIVSRGRGGPPGQFHDNANGGQNGTVQEIMIPAGKAGLVIGKGGETIKQLQERAGVKMILIQDGSQNTNVDKPLRIIGDPYKVQQACEMVMDILRERDQGGFGDRNEYGSRIGGGIDVPVPRHSVGVVIGRSGEMIKKIQNDAGVRIQFKQDDGTGPEKIAHIMGPPDRCEHAARIINDLLQSLRSGPPGPPGGPGMPPGGRGRGRGQGNWGPPGGEMTFSIPTHKCGLVIGRGGENVKAINQQTGAFVEISRQLPPNGDPNFKLFIIRGSPQQIDHAKQLIEEKIEGPLCPVGPGPGGPGPAGPMGPFNPGPFNQGPPGAPPHAGGPPPHQYPPQGWGNTYPQWQPPAPHDPSKAAAAAADPNAAWAAYYSHYYQQPPGPVPGPAPAPAAPPAQGEPPQPPPTGQSDYTKAWEEYYKKIGQQPQQPGAPPQQDYTKAWEEYYKKQAQVATGGGPGAPPGSQPDYSAAWAEYYRQQAAYYGQTPGPGGPQPPPTQQGQQQAQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92945 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T6 Phosphorylation
S54 Phosphorylation
K71 Methylation
K71 Ubiquitination
K87 Acetylation
K87 Ubiquitination
T94 Phosphorylation
T95 Phosphorylation
S99 Phosphorylation
T100 Phosphorylation
K109 Acetylation
K109 Methylation
K109 Ubiquitination
S120 Phosphorylation
K121 Acetylation
K121 Sumoylation
K121 Ubiquitination
K122 Acetylation
K122 Ubiquitination
S125 Phosphorylation
S129 Phosphorylation
S131 Phosphorylation
S132 Phosphorylation Q13315 (ATM)
S144 Phosphorylation
Y149 Phosphorylation
R162 Methylation
K169 Ubiquitination
S174 Phosphorylation
C176 S-Nitrosylation
K177 Ubiquitination
S181 Phosphorylation
S184 Phosphorylation
S191 Phosphorylation
S193 Phosphorylation P31749 (AKT1) , P31751 (AKT2)
T195 Phosphorylation
S200 Phosphorylation
K203 Ubiquitination
K205 Ubiquitination
T234 Phosphorylation
K251 Ubiquitination
T255 Phosphorylation
K257 Ubiquitination
S274 Phosphorylation Q13315 (ATM)
T277 Phosphorylation
K281 Ubiquitination
R314 Methylation
Y317 Phosphorylation P12931 (SRC)
S319 Phosphorylation
S333 Phosphorylation
R340 Methylation
S341 Phosphorylation
K347 Ubiquitination
R355 Methylation
K359 Sumoylation
K359 Ubiquitination
R378 Methylation
R394 Methylation
R411 Methylation
R413 Methylation
R415 Methylation
C436 S-Nitrosylation
R442 Methylation
K448 Methylation
K448 Ubiquitination
S461 Phosphorylation
R478 Methylation
S480 Phosphorylation
K488 Acetylation
K488 Ubiquitination
Y583 Phosphorylation P12931 (SRC)
Y625 Phosphorylation
Y626 Phosphorylation
K627 Acetylation
K628 Ubiquitination
Y644 Phosphorylation
K646 Ubiquitination
Y651 Phosphorylation
Y652 Phosphorylation
K653 Acetylation
K654 Ubiquitination
S670 Phosphorylation Q13315 (ATM)
Y674 Phosphorylation
Y681 Phosphorylation
Y688 Phosphorylation P12931 (SRC)
T692 Phosphorylation Q15759 (MAPK11) , Q16539 (MAPK14)

Research Backgrounds

Function:

Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

PTMs:

Phosphorylation at Ser-193 leads to the unfolding of the unstable KH domain 1, creating a site for 14-3-3 YWHAZ binding, which promotes nuclear localization and impairs the RNA degradation function.

Subcellular Location:

Nucleus. Cytoplasm.
Note: A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in neural and non-neural cell lines.

Subunit Structure:

Part of a ternary complex containing FUBP2, PTBP1, PTBP2 and HNRPH1. Interacts with PARN. Interacts with PQBP1.

Family&Domains:

KH domains KH 3 and KH 4 behave as independent binding modules and can interact with different regions of the AU-rich RNA targets of degradation.

Belongs to the KHSRP family.

Restrictive clause

 

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