ETAA1 Antibody - #DF9512

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Product: ETAA1 Antibody
Catalog: DF9512
Description: Rabbit polyclonal antibody to ETAA1
Application: WB IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 103 kDa; 103kD(Calculated).
Uniprot: Q9NY74
RRID: AB_2842708

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
ETAA1 Antibody detects endogenous levels of total ETAA1.
RRID:
AB_2842708
Cite Format: Affinity Biosciences Cat# DF9512, RRID:AB_2842708.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Etaa1; ETAA1_HUMAN; ETAA16; Ewing''s tumor-associated antigen 1; Ewing''s tumor-associated antigen 16;

Immunogens

Immunogen:
Uniprot:

Q9NY74(ETAA1_HUMAN) >>Visit HPA database.

Gene(ID):
ETAA1(54465)
Expression:
Q9NY74 ETAA1_HUMAN:

Expressed at high levels in the brain, liver kidney and Ewing tumor cell lines.

Sequence:
MSRRRKHDDSPSPKKTPHKTVAAEECGSVVEPGRRRLRSARGSWPCGAREGPPGPVRQREQPPTAALCSKSNPEERYETPKRALKMDSLSSSFSSPNDPDGQNDIFWDQNSPLTKQLGKGRKKQIYTTDSDEISHIVNRIAPQDEKPTTNSMLDMWIGETAIPCTPSVAKGKSRAKISCTKLKTQSQEEELMKLAKQFDKNMEELDVIQEQNKRNYDFTQMISETEILSNYKDNIQMWSLHNIVPEIDNATKKPIKGNTKISVANNQNSSQKPFDQIAEAAFNAIFDGSTQKCSGQLSQELPEAFWSTSNTTFVKTNALKEEKIITNETLVIEKLSNKTPRSLSSQVDTPIMTKSCVTSCTKEPETSNKYIDAFTTSDFEDDWENLLGSEPFAMQNIDMPELFPSKTAHVTDQKEICTFNSKTVKNTSRANTSPDARLGDSKVLQDLSSKTYDRELIDAEYRFSPNSNKSNKLSTGNKMKFENSSNKIVIQDEIQNCIVTSNLTKIKEDILTNSTEASERKSALNTRYSNEQKNKCILNQSIKAPVNTDLFGSANLGSKTSVSNPNQTSASKVGSFFDDWNDPSFANEIIKACHQLDNTWEADDVDDDLLYQACDDIERLTQQQDIRKDSKTSESICEINNNSEHGAKLTQQQDIRKDSKTSESICEINNNSEHGAKNMFAISKQGSNLVQSKHLNPGSISVQTSLTNSSQIDKPMKMEKGEMYGNSPRFLGATNLTMYSKISNCQINNLHVSYTNTDVPIQVNSSKLVLPGSSSLNVTSDHMNTEITTYKKKLSTNQPCHKTVTDEAQSNLNTTVGFSKFTFTRMKNSQILSQFNQNCITGSMSDTKITQGVEKKKGVNPLLEEAVGQQSLVKLSESLKQSSKEEEEKNRKCSPEEIQRKRQEALVRRMAKARASSVNAAPTSFL

PTMs - Q9NY74 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
S12 Phosphorylation
S43 Phosphorylation
K119 Methylation
K123 Ubiquitination
S130 Phosphorylation
K146 Acetylation
S178 Phosphorylation
K183 Sumoylation
K183 Ubiquitination
T184 Phosphorylation
S186 Phosphorylation
K193 Ubiquitination
K196 Sumoylation
K200 Ubiquitination
K213 Ubiquitination
K256 Acetylation
K260 Sumoylation
S298 Phosphorylation
S342 Phosphorylation
S344 Phosphorylation
S345 Phosphorylation
T349 Phosphorylation
K354 Ubiquitination
S359 Phosphorylation
K362 Ubiquitination
K414 Sumoylation
K414 Ubiquitination
S433 Phosphorylation
K442 Sumoylation
K442 Ubiquitination
K450 Ubiquitination
S464 Phosphorylation
K469 Ubiquitination
K507 Sumoylation
K507 Ubiquitination
Y528 Phosphorylation
S529 Phosphorylation
K535 Sumoylation
K535 Ubiquitination
S541 Phosphorylation
K543 Sumoylation
K543 Ubiquitination
S553 Phosphorylation
K559 Ubiquitination
K572 Ubiquitination
T599 Phosphorylation
K631 Sumoylation
K648 Ubiquitination
K660 Sumoylation
K684 Sumoylation
K684 Ubiquitination
K717 Sumoylation
K720 Ubiquitination
S727 Phosphorylation
S795 Phosphorylation
T796 Phosphorylation
K802 Ubiquitination
S833 Phosphorylation
K857 Ubiquitination
K874 Ubiquitination
K880 Ubiquitination
R891 Methylation
S894 Phosphorylation

Research Backgrounds

Function:

Replication stress response protein that accumulates at DNA damage sites and promotes replication fork progression and integrity. Recruited to stalled replication forks via interaction with the RPA complex and directly stimulates ATR kinase activity independently of TOPBP1. Probably only regulates a subset of ATR targets.

PTMs:

Phosphorylated by ATR.

Subcellular Location:

Nucleus.
Note: Localizes at sites of DNA damage following replication stress (PubMed:27601467, PubMed:27723720, PubMed:27723717). Recruited to stalled replication forks via interaction with RPA1 and RPA2 subunits of the RPA complex (PubMed:27601467, PubMed:27723720).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at high levels in the brain, liver kidney and Ewing tumor cell lines.

Subunit Structure:

Interacts (via RBM1 motif) with RPA1. Interacts (via RBM2 motif) with RPA2. Interacts (via the ATR-activation domain motif) with ATR.

Family&Domains:

The RBM1 (RPA1-binding, also named RPA70N-binding) motif mediates interaction with RPA1 (PubMed:27601467, PubMed:27723720, PubMed:27723717). The RBM2 (RPA2-binding, also named RPA32C-binding) motif mediates interaction with RPA2 (PubMed:27601467, PubMed:27723720, PubMed:27723717).

The ATR-activation domain (AAD) motif is required to bind and activate ATR (PubMed:27723720, PubMed:27723717).

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