EIF2C3 Antibody - #DF9508

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Product: EIF2C3 Antibody
Catalog: DF9508
Description: Rabbit polyclonal antibody to EIF2C3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 97 kDa; 97kD(Calculated).
Uniprot: Q9H9G7
RRID: AB_2842704

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(83%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
EIF2C3 Antibody detects endogenous levels of total EIF2C3.
RRID:
AB_2842704
Cite Format: Affinity Biosciences Cat# DF9508, RRID:AB_2842704.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5730550L01Rik; Ago 3; Ago3; AGO3_HUMAN; argonaute 3; Argonaute3; eIF 2C 3; eIF-2C 3; eIF2C 3; Eif2c3; EIF2C3 protein; Eukaryotic translation initiation factor 2C 3; Eukaryotic translation initiation factor 2C3; FLJ12765; hAgo3; MGC86946; Protein argonaute-3;

Immunogens

Immunogen:
Uniprot:

Q9H9G7(AGO3_HUMAN) >>Visit HPA database.

Gene(ID):
AGO3(192669)
Sequence:
MEIGSAGPAGAQPLLMVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSIKFVSRVSWHLLHEVLTGRTLPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
83
Chicken
83
Pig
57
Horse
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H9G7 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
K28 Ubiquitination
K89 Ubiquitination
K174 Ubiquitination
Y189 Phosphorylation
T275 Phosphorylation
Y317 Phosphorylation
Y323 Phosphorylation
K336 Ubiquitination
T338 Phosphorylation
Y339 Phosphorylation
K356 Ubiquitination
S363 Phosphorylation
K367 Ubiquitination
Y421 Phosphorylation
T445 Phosphorylation
K480 Ubiquitination
K494 Ubiquitination
T512 Phosphorylation
Y513 Phosphorylation
S514 Phosphorylation
T527 Phosphorylation
K534 Ubiquitination
K656 Ubiquitination
Y682 Phosphorylation
Y683 Phosphorylation
Y705 Phosphorylation
S825 Phosphorylation
S829 Phosphorylation
S832 Phosphorylation
S835 Phosphorylation

Research Backgrounds

Function:

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.

Subcellular Location:

Cytoplasm>P-body.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with EIF4B, IMP8, PRMT5 and TNRC6B. Interacts with APOBEC3F, APOBEC3G and APOBEC3H. Interacts with EDC4.

Family&Domains:

Belongs to the argonaute family. Ago subfamily.

Restrictive clause

 

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