ENDOD1 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from rat muscle, using ENDOD1 Antibody.

DF9497 at 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P.

Product:	ENDOD1 Antibody
Catalog:	DF9497
Description:	Rabbit polyclonal antibody to ENDOD1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	55 kDa; 55kD(Calculated).
Uniprot:	O94919
RRID:	AB_2842693

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(82%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(91%)

Clonality:

Polyclonal

Specificity:

ENDOD1 Antibody detects endogenous levels of total ENDOD1.

RRID:

AB_2842693
Cite Format: Affinity Biosciences Cat# DF9497, RRID:AB_2842693.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C85344; ENDD1_HUMAN; ENDOD1; Endonuclease domain containing 1; Endonuclease domain-containing 1 protein; KIAA0830;

Immunogens

Immunogen:

Uniprot:

O94919(ENDD1_HUMAN) >>Visit HPA database.

Gene(ID):

ENDOD1(23052)

Sequence:

O94919 ENDD1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGTARWLALGSLFALAGLLEGRLVGEEEAGFGECDKFFYAGTPPAGLAADSHVKICQRAEGAERFATLYSTRDRIPVYSAFRAPRPAPGGAEQRWLVEPQIDDPNSNLEEAINEAEAITSVNSLGSKQALNTDYLDSDYQRGQLYPFSLSSDVQVATFTLTNSAPMTQSFQERWYVNLHSLMDRALTPQCGSGEDLYILTGTVPSDYRVKDKVAVPEFVWLAACCAVPGGGWAMGFVKHTRDSDIIEDVMVKDLQKLLPFNPQLFQNNCGETEQDTEKMKKILEVVNQIQDEERMVQSQKSSSPLSSTRSKRSTLLPPEASEGSSSFLGKLMGFIATPFIKLFQLIYYLVVAILKNIVYFLWCVTKQVINGIESCLYRLGSATISYFMAIGEELVSIPWKVLKVVAKVIRALLRILCCLLKAICRVLSIPVRVLVDVATFPVYTMGAIPIVCKDIALGLGGTVSLLFDTAFGTLGGLFQVVFSVCKRIGYKVTFDNSGEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Pig

Sheep

Dog

Rabbit

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O94919 As Substrate

O94919

Site	PTM Type	Source
S137	Phosphorylation	Uniprot
Y207	Phosphorylation	Uniprot
K300	Ubiquitination	Uniprot
S303	Phosphorylation	Uniprot
S306	Phosphorylation	Uniprot
S307	Phosphorylation	Uniprot
S310	Phosphorylation	Uniprot
S313	Phosphorylation	Uniprot
T314	Phosphorylation	Uniprot
K407	Acetylation	Uniprot
Y490	Phosphorylation	Uniprot

Research Backgrounds

O94919_ENDD1_HUMAN

Function:

May act as a DNase and a RNase.

Subcellular Location:

Secreted.

Family&Domains:

Belongs to the DNA/RNA non-specific endonuclease family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ENDOD1 Antibody - #DF9497