ELP3 Antibody - #DF9496
| Product: | ELP3 Antibody |
| Catalog: | DF9496 |
| Description: | Rabbit polyclonal antibody to ELP3 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 62 kDa; 62kD(Calculated). |
| Uniprot: | Q9H9T3 |
| RRID: | AB_2842692 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9496, RRID:AB_2842692.
Fold/Unfold
DKFZp761L098; Elongation protein 3 homolog (S. cerevisiae); Elongation protein 3 homolog; ELONGATION PROTEIN 3, S. CEREVISIAE, HOMOLOG OF; elongator acetyltransferase complex subunit 3; Elongator complex protein 3; elp3; ELP3_HUMAN; FLJ10422; hELP3; Hypothetical protein DKFZp761L098; Kat9;
Immunogens
- Q9H9T3 ELP3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRQKRKGDLSPAELMMLTIGDVIKQLIEAHEQGKDIDLNKVKTKTAAKYGLSAQPRLVDIIAAVPPQYRKVLMPKLKAKPIRTASGIAVVAVMCKPHRCPHISFTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPFLQTRHRIEQLKQLGHSVDKVEFIVMGGTFMALPEEYRDYFIRNLHDALSGHTSNNIYEAVKYSERSLTKCIGITIETRPDYCMKRHLSDMLTYGCTRLEIGVQSVYEDVARDTNRGHTVKAVCESFHLAKDSGFKVVAHMMPDLPNVGLERDIEQFTEFFENPAFRPDGLKLYPTLVIRGTGLYELWKSGRYKSYSPSDLVELVARILALVPPWTRVYRVQRDIPMPLVSSGVEHGNLRELALARMKDLGIQCRDVRTREVGIQEIHHKVRPYQVELVRRDYVANGGWETFLSYEDPDQDILIGLLRLRKCSEETFRFELGGGVSIVRELHVYGSVVPVSSRDPTKFQHQGFGMLLMEEAERIAREEHGSGKIAVISGVGTRNYYRKIGYRLQGPYMVKMLK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H9T3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Ubiquitination | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| T197 | Phosphorylation | Uniprot | |
| Y202 | Phosphorylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K229 | Methylation | Uniprot | |
| Y251 | Phosphorylation | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K280 | Acetylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| Y318 | Phosphorylation | Uniprot | |
| Y329 | Phosphorylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| T360 | Phosphorylation | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| R424 | Methylation | Uniprot | |
| Y427 | Phosphorylation | Uniprot | |
| K491 | Ubiquitination | Uniprot | |
| K517 | Ubiquitination | Uniprot |
Research Backgrounds
Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. The elongator complex is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase. Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation. Required for acetylation of GJA1 in the developing cerebral cortex (By similarity).
Cytoplasm. Nucleus.
Nucleus.
Cytoplasm. Nucleus.
Expressed in the cerebellum and spinal motor neurons.
Component of the RNA polymerase II elongator complex (Elongator), which consists of ELP1, STIP1/ELP2, ELP3, ELP4, ELP5 and ELP6. ELP1, STIP1/ELP2 and ELP3 form the Elongator core complex. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit. Interacts with ELP1. Interacts with alpha-tubulin.
Belongs to the ELP3 family.
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