Product: GFM1 Antibody
Catalog: DF9494
Description: Rabbit polyclonal antibody to GFM1
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Zebrafish, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 83 kDa; 83kD(Calculated).
Uniprot: Q96RP9
RRID: AB_2842690

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
GFM1 Antibody detects endogenous levels of total GFM1.
RRID:
AB_2842690
Cite Format: Affinity Biosciences Cat# DF9494, RRID:AB_2842690.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

COXPD1; EF-Gmt; EFG; EFG1; EFGM; EFGM_HUMAN; EGF1; Elongation factor G 1; Elongation factor G 1 mitochondrial; Elongation factor G; Elongation factor G1; FLJ12662; FLJ13632; FLJ20773; G elongation factor mitochondrial 1; G translation elongation factor mitochondrial; GFM 1; GFM; gfm1; hEFG1; mEF G 1; mEF-G 1; mEFG 1; mitochondrial; Mitochondrial elongation factor G1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MRLLGAAAVAALGRGRAPASLGWQRKQVNWKACRWSSSGVIPNEKIRNIGISAHIDSGKTTLTERVLYYTGRIAKMHEVKGKDGVGAVMDSMELERQRGITIQSAATYTMWKDVNINIIDTPGHVDFTIEVERALRVLDGAVLVLCAVGGVQCQTMTVNRQMKRYNVPFLTFINKLDRMGSNPARALQQMRSKLNHNAAFMQIPMGLEGNFKGIVDLIEERAIYFDGDFGQIVRYGEIPAELRAAATDHRQELIECVANSDEQLGEMFLEEKIPSISDLKLAIRRATLKRSFTPVFLGSALKNKGVQPLLDAVLEYLPNPSEVQNYAILNKEDDSKEKTKILMNSSRDNSHPFVGLAFKLEVGRFGQLTYVRSYQGELKKGDTIYNTRTRKKVRLQRLARMHADMMEDVEEVYAGDICALFGIDCASGDTFTDKANSGLSMESIHVPDPVISIAMKPSNKNDLEKFSKGIGRFTREDPTFKVYFDTENKETVISGMGELHLEIYAQRLEREYGCPCITGKPKVAFRETITAPVPFDFTHKKQSGGAGQYGKVIGVLEPLDPEDYTKLEFSDETFGSNIPKQFVPAVEKGFLDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEISFIRAGEGALKQALANATLCILEPIMAVEVVAPNEFQGQVIAGINRRHGVITGQDGVEDYFTLYADVPLNDMFGYSTELRSCTEGKGEYTMEYSRYQPCLPSTQEDVINKYLEATGQLPVKKGKAKN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Bovine
79
Sheep
79
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96RP9 As Substrate

Site PTM Type Enzyme
R14 Methylation
Y68 Phosphorylation
Y69 Phosphorylation
K82 Ubiquitination
S91 Phosphorylation
T121 Phosphorylation
T128 Phosphorylation
Y165 Phosphorylation
K175 Acetylation
Y224 Phosphorylation
S275 Phosphorylation
K280 Ubiquitination
S291 Phosphorylation
T293 Phosphorylation
S345 Phosphorylation
R372 Methylation
K380 Ubiquitination
K468 Acetylation
K551 Ubiquitination
Y564 Phosphorylation
T565 Phosphorylation
K566 Ubiquitination
K580 Ubiquitination
Y735 Phosphorylation
T739 Phosphorylation

Research Backgrounds

Function:

Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.

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