UBR2 Antibody - #DF9491
(2)
(3)
| Product: | UBR2 Antibody |
| Catalog: | DF9491 |
| Description: | Rabbit polyclonal antibody to UBR2 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 201kDa; 201kD(Calculated). |
| Uniprot: | Q8IWV8 |
| RRID: | AB_2842687 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9491, RRID:AB_2842687.
Fold/Unfold
bA49A4.1; C6orf133; dJ242G1.1; dJ392M17.3; DKFZp686C08114; E3 ubiquitin protein ligase UBR2; KIAA0349; MGC71112; N recognin 2; OTTHUMP00000016403; OTTHUMP00000039767; OTTHUMP00000039768; RP3 392M17.3; Ubiquitin ligase E3 alpha II; Ubiquitin protein ligase E3 alpha 2; Ubiquitin protein ligase E3 alpha II; Ubiquitin protein ligase E3 component n recognin 2; UBR 2;
Immunogens
Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level).
- Q8IWV8 UBR2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASELEPEVQAIDRSLLECSAEEIAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAITFRYAVEILTWEKESELPADLEMVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLSDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYQQLQRDFMEDDHERAVSVTALSVQFFTAPTLARMLITEENLMSIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDELRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCASDEKVLIEAYKKCLAVLMQCHGGYTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDVVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMLVGERFSPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMGTILQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLENVTEEHVVTFTFTQKISKPGEAPKNSPSILAMLETLQNAPYLEVHKDMIRWILKTFNAVKKMRESSPTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDHSPVASDMTLTALGPAQTQVPEQRQFVTCILCQEEQEVKVESRAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTSSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRNIFNNRLNFSDQPNLTQWIRTISQQIKALQFLRKEESTPNNASTKNSENVDELQLPEGFRPDFRPKIPYSESIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVASVSVVQGHFCKLFASLVPNDSHEELPCILDIDMFHLLVGLVLAFPALQCQDFSGISLGTGDLHIFHLVTMAHIIQILLTSCTEENGMDQENPPCEEESAVLALYKTLHQYTGSALKEIPSGWHLWRSVRAGIMPFLKCSALFFHYLNGVPSPPDIQVPGTSHFEHLCSYLSLPNNLICLFQENSEIMNSLIESWCRNSEVKRYLEGERDAIRYPRESNKLINLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGSLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSVTEEIGHAQEANQTLVGIDWQHL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IWV8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| Y274 | Phosphorylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| T581 | Phosphorylation | Uniprot | |
| S734 | Phosphorylation | Uniprot | |
| K779 | Ubiquitination | Uniprot | |
| K789 | Ubiquitination | Uniprot | |
| K798 | Ubiquitination | Uniprot | |
| K821 | Ubiquitination | Uniprot | |
| K822 | Ubiquitination | Uniprot | |
| T826 | Phosphorylation | Uniprot | |
| Y844 | Phosphorylation | Uniprot | |
| K994 | Ubiquitination | Uniprot | |
| S1005 | Phosphorylation | Uniprot | |
| S1006 | Phosphorylation | Uniprot | |
| T1008 | Phosphorylation | Uniprot | |
| S1009 | Phosphorylation | Uniprot | |
| T1014 | Phosphorylation | Uniprot | |
| T1017 | Phosphorylation | Uniprot | |
| S1022 | Phosphorylation | Uniprot | |
| K1044 | Ubiquitination | Uniprot | |
| S1050 | Phosphorylation | Uniprot | |
| K1142 | Ubiquitination | Uniprot | |
| K1187 | Ubiquitination | Uniprot | |
| K1255 | Ubiquitination | Uniprot | |
| T1266 | Phosphorylation | Uniprot | |
| K1273 | Ubiquitination | Uniprot | |
| K1294 | Ubiquitination | Uniprot | |
| T1312 | Phosphorylation | Uniprot | |
| K1599 | Ubiquitination | Uniprot | |
| Y1607 | Phosphorylation | Uniprot | |
| K1621 | Ubiquitination | Uniprot | |
| K1689 | Ubiquitination | Uniprot | |
| K1718 | Ubiquitination | Uniprot |
Research Backgrounds
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Plays a critical role in chromatin inactivation and chromosome-wide transcriptional silencing during meiosis via ubiquitination of histone H2A. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth. Required for spermatogenesis, promotes, with Tex19.1, SPO11-dependent recombination foci to accumulate and drive robust homologous chromosome synapsis (By similarity). Polyubiquitinates LINE-1 retrotransposon encoded, LIRE1, which induces degradation, inhibiting LINE-1 retrotransposon mobilization (By similarity).
Nucleus.
Note: Associated with chromatin during meiosis.
Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level).
Interacts with UBE2B; promotes the UBE2B-H2A interaction and the ubiquitination of histone H2A by UBE2B and UBR2 (By similarity). Interacts with RECQL4. Interacts with TEX19; does not lead to TEX19 degradation and stabilizes it (By similarity). Interacts with CASP8. Interacts with ATXN3.
The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.
The UBR-type zinc finger forms a pocket that mediates recognition of type 1 N-degrons. It exhibits preference for Arginine in first position, has poor affinity for histidine, and doesn't bind acetylated peptides.
Belongs to the UBR1 family.
References
Application: WB Species: Mouse Sample:
Application: WB Species: Mouse Sample:
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