PJA1 Antibody - #DF9487

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Product: PJA1 Antibody
Catalog: DF9487
Description: Rabbit polyclonal antibody to PJA1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 71 kDa; 71kD(Calculated).
Uniprot: Q8NG27
RRID: AB_2842683

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
PJA1 Antibody detects endogenous levels of total PJA1.
RRID:
AB_2842683
Cite Format: Affinity Biosciences Cat# DF9487, RRID:AB_2842683.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:

Q8NG27(PJA1_HUMAN) >>Visit HPA database.

Gene(ID):
PJA1(64219)
Expression:
Q8NG27 PJA1_HUMAN:

Expressed in various regions of the brain including the cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Highest levels in the cerebral cortex.

Sequence:
MGQESSKPVWPNPTGGYQSNTGRRYGRRHAYVSFRPPTSQRERIASQRKTNSEVPMHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSRYPPREYRASGSRRGMAYGHIDSYGADDSEEEGAGPVERPPVRGKTGKFKDDKLYDPEKGARSLAGPPPHFSSFSRDVREERDKLDPVPAARCSASRADFLPQSSVASQSSSEGKLATKGDSSERERREQNLPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFDTDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

PTMs - Q8NG27 As Substrate

Site PTM Type Enzyme
S68 Phosphorylation
S70 Phosphorylation
S78 Phosphorylation
T87 Phosphorylation
S126 Phosphorylation
S265 Phosphorylation
T277 Phosphorylation
S312 Phosphorylation
S313 Phosphorylation
S314 Phosphorylation
Y316 Phosphorylation
K337 Ubiquitination
T344 Phosphorylation
S354 Phosphorylation
Y360 Phosphorylation
S365 Phosphorylation
S367 Phosphorylation
S387 Phosphorylation
S388 Phosphorylation
S389 Phosphorylation
S392 Phosphorylation
Y440 Phosphorylation
S448 Phosphorylation
S557 Phosphorylation
K628 Ubiquitination

Research Backgrounds

Function:

Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting.

PTMs:

Substrate for E2-dependent ubiquitination.

Tissue Specificity:

Expressed in various regions of the brain including the cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Highest levels in the cerebral cortex.

Subunit Structure:

Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2.

Family&Domains:

The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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