MARCH7 Antibody - #DF9483

(3)  
Product: MARCH7 Antibody
Catalog: DF9483
Description: Rabbit polyclonal antibody to MARCH7
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Rabbit, Chicken, Xenopus
Mol.Wt.: 78 kDa; 78kD(Calculated).
Uniprot: Q9H992
RRID: AB_2842679

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MARCH7 Antibody detects endogenous levels of total MARCH7.
RRID:
AB_2842679
Cite Format: Affinity Biosciences Cat# DF9483, RRID:AB_2842679.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AXO; AXOT; Axotrophin; E3 ubiquitin-protein ligase MARCH7; MARCH VII; MARCH-VII; March7; MARH7_HUMAN; Membrane associated RING CH protein VII; Membrane associated ring finger (C3HC4) 7 E3 ubiquitin protein ligase; membrane associated ring finger C3HC4 7; Membrane associated RING finger protein 7; Membrane-associated RING finger protein 7; Membrane-associated RING-CH protein VII; RING finger protein 177; RNF177;

Immunogens

Immunogen:
Uniprot:

Q9H992(MARH7_HUMAN) >>Visit HPA database.

Gene(ID):
MARCH7(64844)
Sequence:
MESKPSRIPRRISVQPSSSLSARMMSGSRGSSLNDTYHSRDSSFRLDSEYQSTSASASASPFQSAWYSESEITQGARSRSQNQQRDHDSKRPKLSCTNCTTSAGRNVGNGLNTLSDSSWRHSQVPRSSSMVLGSFGTDLMRERRDLERRTDSSISNLMDYSHRSGDFTTSSYVQDRVPSYSQGARPKENSMSTLQLNTSSTNHQLPSEHQTILSSRDSRNSLRSNFSSRESESSRSNTQPGFSYSSSRDEAPIISNSERVVSSQRPFQESSDNEGRRTTRRLLSRIASSMSSTFFSRRSSQDSLNTRSLNSENSYVSPRILTASQSRSNVPSASEVPDNRASEASQGFRFLRRRWGLSSLSHNHSSESDSENFNQESEGRNTGPWLSSSLRNRCTPLFSRRRREGRDESSRIPTSDTSSRSHIFRRESNEVVHLEAQNDPLGAAANRPQASAASSSATTGGSTSDSAQGGRNTGISGILPGSLFRFAVPPALGSNLTDNVMITVDIIPSGWNSADGKSDKTKSAPSRDPERLQKIKESLLLEDSEEEEGDLCRICQMAAASSSNLLIEPCKCTGSLQYVHQDCMKKWLQAKINSGSSLEAVTTCELCKEKLELNLEDFDIHELHRAHANEQAEYEFISSGLYLVVLLHLCEQSFSDMMGNTNEPSTRVRFINLARTLQAHMEDLETSEDDSEEDGDHNRTFDIA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Xenopus
100
Chicken
100
Rabbit
100
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H992 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K4 Ubiquitination
S13 Phosphorylation
S26 Phosphorylation
S28 Phosphorylation
R29 Methylation
S31 Phosphorylation
T36 Phosphorylation
Y37 Phosphorylation
K93 Ubiquitination
S102 Phosphorylation
S127 Phosphorylation
S129 Phosphorylation
S179 Phosphorylation
S218 Phosphorylation
S221 Phosphorylation
T238 Phosphorylation
S243 Phosphorylation
S245 Phosphorylation
S271 Phosphorylation
S284 Phosphorylation
S288 Phosphorylation
S300 Phosphorylation
S314 Phosphorylation
Y315 Phosphorylation
S317 Phosphorylation
S324 Phosphorylation
S359 Phosphorylation
S368 Phosphorylation
S389 Phosphorylation
T395 Phosphorylation
R406 Methylation
S428 Phosphorylation
K522 Ubiquitination
K536 Ubiquitination
S544 Phosphorylation
K585 Ubiquitination
K586 Ubiquitination
K591 Ubiquitination
S597 Phosphorylation
K608 Ubiquitination
K610 Ubiquitination
T686 Phosphorylation
S687 Phosphorylation
S691 Phosphorylation

Research Backgrounds

Function:

E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May be involved in T-cell proliferation by regulating LIF secretion (By similarity). May play a role in lysosome homeostasis.

Family&Domains:

The RING-CH-type zinc finger domain is required for E3 ligase activity.

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