HECTD1 Antibody - #DF9478

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Product: HECTD1 Antibody
Catalog: DF9478
Description: Rabbit polyclonal antibody to HECTD1
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 289kDa; 289kD(Calculated).
Uniprot: Q9ULT8
RRID: AB_2842674

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
HECTD1 Antibody detects endogenous levels of total HECTD1.
RRID:
AB_2842674
Cite Format: Affinity Biosciences Cat# DF9478, RRID:AB_2842674.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E3 ligase for inhibin receptor; E3 ubiquitin protein ligase HECTD1; E3 ubiquitin-protein ligase HECTD1; EULIR; HECD1_HUMAN; HECT domain containing protein 1; HECT domain-containing protein 1; HECTD1; KIAA1131;

Immunogens

Immunogen:
Uniprot:

Q9ULT8(HECD1_HUMAN) >>Visit HPA database.

Gene(ID):
HECTD1(25831)
Sequence:
MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTTADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTILVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSMDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPDTVASPKPVSSTVSGTTQSWSSLVKNNCPDKTSAAAGSSSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVPQTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTPGESSAISMGIVSVSSPDVSSVSELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEDGDEQLQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAIKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULT8 As Substrate

Site PTM Type Enzyme
R114 Methylation
S182 Phosphorylation
R216 Methylation
K229 Ubiquitination
T233 Phosphorylation
T246 Phosphorylation
S248 Phosphorylation
S251 Phosphorylation
S252 Phosphorylation
K255 Ubiquitination
S259 Phosphorylation
T260 Phosphorylation
S270 Phosphorylation
K340 Ubiquitination
S342 Phosphorylation
T346 Phosphorylation
R353 Methylation
S357 Phosphorylation
S358 Phosphorylation
S362 Phosphorylation
T445 Phosphorylation
K461 Ubiquitination
T462 Phosphorylation
K466 Ubiquitination
S481 Phosphorylation
K491 Ubiquitination
K522 Ubiquitination
S544 Phosphorylation
T626 Phosphorylation
S631 Phosphorylation
S632 Phosphorylation
S667 Phosphorylation
Y706 Phosphorylation
S715 Phosphorylation
S717 Phosphorylation
K727 Ubiquitination
K736 Ubiquitination
K782 Ubiquitination
K825 Acetylation
K828 Acetylation
K846 Ubiquitination
T858 Phosphorylation
K947 Ubiquitination
Y1034 Phosphorylation
K1041 Ubiquitination
Y1044 Phosphorylation
Y1111 Phosphorylation
S1119 Phosphorylation
K1253 Ubiquitination
K1272 Ubiquitination
Y1273 Phosphorylation
K1287 Ubiquitination
T1342 Phosphorylation
S1345 Phosphorylation
T1357 Phosphorylation
K1371 Ubiquitination
S1373 Phosphorylation
K1382 Ubiquitination
S1384 Phosphorylation
S1385 Phosphorylation
S1386 Phosphorylation
S1387 Phosphorylation
S1390 Phosphorylation
S1393 Phosphorylation
S1394 Phosphorylation
K1463 Ubiquitination
S1468 Phosphorylation
T1472 Phosphorylation
S1487 Phosphorylation
S1488 Phosphorylation
K1500 Ubiquitination
S1509 Phosphorylation
S1510 Phosphorylation
S1511 Phosphorylation
S1520 Phosphorylation
S1531 Phosphorylation
S1533 Phosphorylation
T1558 Phosphorylation
T1561 Phosphorylation
S1567 Phosphorylation
S1570 Phosphorylation
S1571 Phosphorylation
T1575 Phosphorylation
T1577 Phosphorylation
Y1657 Phosphorylation
Y1660 Phosphorylation
S1711 Phosphorylation
Y1751 Phosphorylation
K1754 Ubiquitination
T1760 Phosphorylation
Y1765 Phosphorylation
K1768 Ubiquitination
S1772 Phosphorylation
T1800 Phosphorylation
K1849 Ubiquitination
Y1876 Phosphorylation
K1919 Ubiquitination
K1930 Ubiquitination
K1936 Ubiquitination
S1945 Phosphorylation
K1960 Ubiquitination
T1980 Phosphorylation
K1981 Ubiquitination
K2070 Ubiquitination
K2214 Ubiquitination
K2268 Ubiquitination
K2280 Ubiquitination
K2285 Ubiquitination
S2315 Phosphorylation
S2318 Phosphorylation
K2364 Ubiquitination
S2375 Phosphorylation
K2377 Ubiquitination
S2380 Phosphorylation
K2387 Ubiquitination
K2460 Ubiquitination
K2471 Ubiquitination
K2584 Ubiquitination
K2605 Ubiquitination

Research Backgrounds

Function:

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates 'Lys-63'-linked polyubiquitination of HSP90AA1 which leads to its intracellular localization and reduced secretion. Negatively regulating HSP90AA1 secretion in cranial mesenchyme cells may impair their emigration and may be essential for the correct development of the cranial neural folds and neural tube closure.

Subunit Structure:

Interacts with IGSF1. Interacts (via N-terminus) with HSP90AA1 (By similarity).

Family&Domains:

Belongs to the UPL family. K-HECT subfamily.

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