FANCL Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela cells(heat-shock treatment), using FANCL Antibody.

Product:	FANCL Antibody
Catalog:	DF9476
Description:	Rabbit polyclonal antibody to FANCL
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	43 kDa; 43kD(Calculated).
Uniprot:	Q9NW38
RRID:	AB_2842672

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(89%), Sheep(89%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(89%)

Clonality:

Polyclonal

Specificity:

FANCL Antibody detects endogenous levels of total FANCL.

RRID:

AB_2842672
Cite Format: Affinity Biosciences Cat# DF9476, RRID:AB_2842672.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

E3 ubiquitin-protein ligase FANCL; FAAP43; Fancl; FANCL_HUMAN; Fanconi anemia complementation group L; Fanconi anemia group L protein; Fanconi anemia-associated polypeptide of 43 kDa; FLJ10335; PHD finger protein 9; PHF9; POG; Ubiquitin ligase protein FANCL;

Immunogens

Immunogen:

Uniprot:

Q9NW38(FANCL_HUMAN) >>Visit HPA database.

Gene(ID):

FANCL(55120)

Sequence:

Q9NW38 FANCL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAVTEASLLRQCPLLLPQNRSKTVYEGFISAQGRDFHLRIVLPEDLQLKNARLLCSWQLRTILSGYHRIVQQRMQHSPDLMSFMMELKMLLEVALKNRQELYALPPPPQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQSSLISIYSQFLAAIESLKAFWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENSVLQNLKDVLEIDFPARAILEKSDFTMDCGICYAYQLDGTIPDQVCDNSQCGQPFHQICLYEWLRGLLTSRQSFNIIFGECPYCSKPITLKMSGRKH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Dog

100

Rabbit

100

Horse

Sheep

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NW38 As Substrate

Q9NW38

Site	PTM Type	Source
A2	Acetylation	Uniprot
T4	Phosphorylation	Uniprot
S7	Phosphorylation	Uniprot
K22	Ubiquitination	Uniprot
K49	Ubiquitination	Uniprot
K139	Sumoylation	Uniprot
K153	Ubiquitination	Uniprot
T178	Phosphorylation	Uniprot
K218	Ubiquitination	Uniprot
K261	Ubiquitination	Uniprot
K266	Ubiquitination	Uniprot
S351	Phosphorylation	Uniprot
K364	Ubiquitination	Uniprot

Research Backgrounds

Q9NW38_FANCL_HUMAN

Function:

Ubiquitin ligase protein that mediates monoubiquitination of FANCD2 in the presence of UBE2T, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.

PTMs:

The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.

Subcellular Location:

Cytoplasm. Nucleus.

Subunit Structure:

Interacts with GGN (By similarity). Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. In complex with FANCF, FANCA and FANCG, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins. Interacts with FANCI. Directly interacts (via the RING-type zinc finger) with UBE2T and UBE2W.

Family&Domains:

The UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.

Research Fields

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)

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FANCL Antibody - #DF9476