NSMCE2 Antibody | Affinity Biosciences

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Western blot analysis NSMCE2 using LOVO whole cell lysates.

DF9475 at 1/100 staining rat testis tissue by IHC-P.

Product:	NSMCE2 Antibody
Catalog:	DF9475
Description:	Rabbit polyclonal antibody to NSMCE2
Application:	WB IHC
Reactivity:	Human
Prediction:	Rabbit
Mol.Wt.:	28 kDa; 28kD(Calculated).
Uniprot:	Q96MF7
RRID:	AB_2842671

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Rabbit(100%)

Clonality:

Polyclonal

Specificity:

NSMCE2 Antibody detects endogenous levels of total NSMCE2.

RRID:

AB_2842671
Cite Format: Affinity Biosciences Cat# DF9475, RRID:AB_2842671.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C8orf36; E3 SUMO-protein ligase NSE2; hMMS21; hNSE2; methyl methanesulfonate sensitivity gene 21; MMS21; MMS21 homolog; non-SMC element 2; Non-SMC element 2 homolog; Non-structural maintenance of chromosomes element 2 homolog; NSE2_HUMAN; Nsmce2;

Immunogens

Immunogen:

Uniprot:

Q96MF7(NSE2_HUMAN) >>Visit HPA database.

Gene(ID):

NSMCE2(286053)

Sequence:

Q96MF7 NSE2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPGRSSSNSGSTGFISFSGVESALSSLKNFQACINSGMDTASSVALDLVESQTEVSSEYSMDKAMVEFATLDRQLNHYVKAVQSTINHVKEERPEKIPDLKLLVEKKFLALQSKNSDADFQNNEKFVQFKQQLKELKKQCGLQADREADGTEGVDEDIIVTQSQTNFTCPITKEEMKKPVKNKVCGHTYEEDAIVRMIESRQKRKKKAYCPQIGCSHTDIRKSDLIQDEALRRAIENHNKKRHRHSE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

Pig

Xenopus

Bovine

Sheep

Dog

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96MF7 As Substrate

Q96MF7

Site	PTM Type	Source
M1	Acetylation	Uniprot
S5	Phosphorylation	Uniprot
S6	Phosphorylation	Uniprot
S7	Phosphorylation	Uniprot
S11	Phosphorylation	Uniprot
T12	Phosphorylation	Uniprot
S22	Phosphorylation	Uniprot
S26	Phosphorylation	Uniprot
K80	Ubiquitination	Uniprot
K90	Sumoylation	Uniprot
K90	Ubiquitination	Uniprot
K96	Ubiquitination	Uniprot
K101	Sumoylation	Uniprot
K101	Ubiquitination	Uniprot
K107	Sumoylation	Uniprot
K107	Ubiquitination	Uniprot
S113	Phosphorylation	Uniprot
K114	Ubiquitination	Uniprot
S116	Phosphorylation	Uniprot
K125	Acetylation	Uniprot
K125	Sumoylation	Uniprot
K125	Ubiquitination	Uniprot
K130	Sumoylation	Uniprot
K130	Ubiquitination	Uniprot
K183	Ubiquitination	Uniprot
K207	Ubiquitination	Uniprot
Y209	Phosphorylation	Uniprot
S216	Phosphorylation	Uniprot
K222	Sumoylation	Uniprot
K222	Ubiquitination	Uniprot
S223	Phosphorylation	Uniprot

Research Backgrounds

Q96MF7_NSE2_HUMAN

Function:

E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines. Required for sister chromatid cohesion during prometaphase and mitotic progression.

PTMs:

Sumoylated, possibly via autosumoylation.

Subcellular Location:

Nucleus. Chromosome>Telomere. Nucleus>PML body.
Note: Localizes to PML nuclear bodies in ALT cell lines.

Subunit Structure:

Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NSMCE3.

Family&Domains:

Belongs to the NSE2 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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NSMCE2 Antibody - #DF9475