IGHMBP2 Antibody - #DF9454

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Product: IGHMBP2 Antibody
Catalog: DF9454
Description: Rabbit polyclonal antibody to IGHMBP2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 109kDa; 109kD(Calculated).
Uniprot: P38935
RRID: AB_2842650

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(89%), Horse(100%), Sheep(90%), Dog(80%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
IGHMBP2 Antibody detects endogenous levels of total IGHMBP2.
RRID:
AB_2842650
Cite Format: Affinity Biosciences Cat# DF9454, RRID:AB_2842650.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AEP; Antifreeze enhancer binding protein; ATP-dependent helicase IGHMBP2; Cardiac transcription factor 1; Cardiac transcription factor1; CATF 1; CATF1; CMT2S; DNA-binding protein SMUBP-2; GF-1; Glial factor 1; HCSA; HMN 6; HMN6; IGHMBP 2; Ighmbp2; Immunoglobulin mu binding protein 2; Immunoglobulin mu binding protein2; Immunoglobulin mu-binding protein 2; Immunoglobulin S mu binding protein 2; Immunoglobulin S mu binding protein2; RIPE3 b1; RIPE3b 1; RIPE3b1; SMARD 1; SMARD1; SMBP2_HUMAN; SMUBP 2; SMUBP2; ZFAND7; zinc finger, AN1 type domain 7;

Immunogens

Immunogen:
Uniprot:

P38935(SMBP2_HUMAN) >>Visit HPA database.

Gene(ID):
IGHMBP2(3508)
Expression:
P38935 SMBP2_HUMAN:

Expressed in all tissues examined. Expressed in the developing and adult human brain, with highest expression in the cerebellum. Moderately expressed in fibroblasts.

Sequence:
MASAAVESFVTKQLDLLELERDAEVEERRSWQENISLKELQSRGVCLLKLQVSSQRTGLYGRLLVTFEPRRYGSAAALPSNSFTSGDIVGLYDAANEGSQLATGILTRVTQKSVTVAFDESHDFQLSLDRENSYRLLKLANDVTYRRLKKALIALKKYHSGPASSLIEVLFGRSAPSPASEIHPLTFFNTCLDTSQKEAVLFALSQKELAIIHGPPGTGKTTTVVEIILQAVKQGLKVLCCAPSNIAVDNLVERLALCKQRILRLGHPARLLESIQQHSLDAVLARSDSAQIVADIRKDIDQVFVKNKKTQDKREKSNFRNEIKLLRKELKEREEAAMLESLTSANVVLATNTGASADGPLKLLPESYFDVVVIDECAQALEASCWIPLLKARKCILAGDHKQLPPTTVSHKAALAGLSLSLMERLAEEYGARVVRTLTVQYRMHQAIMRWASDTMYLGQLTAHSSVARHLLRDLPGVAATEETGVPLLLVDTAGCGLFELEEEDEQSKGNPGEVRLVSLHIQALVDAGVPARDIAVVSPYNLQVDLLRQSLVHRHPELEIKSVDGFQGREKEAVILSFVRSNRKGEVGFLAEDRRINVAVTRARRHVAVICDSRTVNNHAFLKTLVEYFTQHGEVRTAFEYLDDIVPENYSHENSQGSSHAATKPQGPATSTRTGSQRQEGGQEAAAPARQGRKKPAGKSLASEAPSQPSLNGGSPEGVESQDGVDHFRAMIVEFMASKKMQLEFPPSLNSHDRLRVHQIAEEHGLRHDSSGEGKRRFITVSKRAPRPRAALGPPAGTGGPAPLQPVPPTPAQTEQPPREQRGPDQPDLRTLHLERLQRVRSAQGQPASKEQQASGQQKLPEKKKKKAKGHPATDLPTEEDFEALVSAAVKADNTCGFAKCTAGVTTLGQFCQLCSRRYCLSHHLPEIHGCGERARAHARQRISREGVLYAGSGTKNGSLDPAKRAQLQRRLDKKLSELSNQRTSRRKERGT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Xenopus
100
Chicken
100
Sheep
90
Zebrafish
89
Dog
80
Bovine
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P38935 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K38 Ubiquitination
S85 Phosphorylation
Y92 Phosphorylation
K138 Ubiquitination
S160 Phosphorylation
T218 Phosphorylation
K237 Ubiquitination
K259 Ubiquitination
S289 Phosphorylation
K298 Ubiquitination
K306 Ubiquitination
K324 Ubiquitination
K394 Acetylation
K394 Ubiquitination
K402 Ubiquitination
S539 Phosphorylation
K572 Ubiquitination
S656 Phosphorylation
T675 Phosphorylation
S677 Phosphorylation
S711 Phosphorylation
S716 Phosphorylation
S722 Phosphorylation
S843 Phosphorylation
S945 Phosphorylation
Y951 Phosphorylation
S954 Phosphorylation
T956 Phosphorylation
S978 Phosphorylation
S986 Phosphorylation

Research Backgrounds

Function:

5' to 3' helicase that unwinds RNA and DNA duplices in an ATP-dependent reaction. Acts as a transcription regulator. Required for the transcriptional activation of the flounder liver-type antifreeze protein gene. Exhibits strong binding specificity to the enhancer element B of the flounder antifreeze protein gene intron. Binds to the insulin II gene RIPE3B enhancer region. May be involved in translation (By similarity). DNA-binding protein specific to 5'-phosphorylated single-stranded guanine-rich sequence related to the immunoglobulin mu chain switch region. Preferentially binds to the 5'-GGGCT-3' motif. Interacts with tRNA-Tyr. Stimulates the transcription of the human neurotropic virus JCV.

Subcellular Location:

Nucleus. Cytoplasm. Cell projection>Axon.
Note: Colocalizes with the translation initiation factor EIF4G2.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues examined. Expressed in the developing and adult human brain, with highest expression in the cerebellum. Moderately expressed in fibroblasts.

Subunit Structure:

Homooligomer. Interacts with RUVBL1, RUVBL2, GTF3C1 and ABT1. Is part of large cytosolic ribonucleoprotein complexes (Probable). Associates with the ribosomes.

Family&Domains:

Belongs to the DNA2/NAM7 helicase family.

References

1). Impaired Local Translation of β-actin mRNA in Ighmbp2-Deficient Motoneurons: Implications for Spinal Muscular Atrophy with respiratory Distress (SMARD1). NEUROSCIENCE, 2018 (PubMed: 29928949) [IF=3.3]

Application: WB    Species: human    Sample: HEK293 cells

Fig. 5:| Ighmbp2 does not directly bind to β-actin mRNA.(A) Western blots of IMP1- and Ighmbp2-IP of RIP were performed to verify the successful pulldown (PD) of IMP1 and Ighmbp2. No direct binding of Ighmbp2 to IMP1 was detectable(lower panel). Negative control conditions (IgG IP) are free of both target proteins, only the heavy chains of rabbit IgG antibodies are detectable, and no protein can be detected when antibodies are omitted (w/o AB).

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