TOP1MT Antibody - #DF9452
| Product: | TOP1MT Antibody |
| Catalog: | DF9452 |
| Description: | Rabbit polyclonal antibody to TOP1MT |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Horse, Sheep, Chicken |
| Mol.Wt.: | 70 kDa; 70kD(Calculated). |
| Uniprot: | Q969P6 |
| RRID: | AB_2842648 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9452, RRID:AB_2842648.
Fold/Unfold
2900052H09Rik; DNA topoisomerase I; DNA topoisomerase I, mitochondrial; mitochondrial; TOP1M_HUMAN; TOP1mt; Topoisomerase I mitochondrial;
Immunogens
- Q969P6 TOP1M_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRVVRLLRLRAALTLLGEVPRRPASRGVPGSRRTQKGSGARWEKEKHEDGVKWRQLEHKGPYFAPPYEPLPDGVRFFYEGRPVRLSVAAEEVATFYGRMLDHEYTTKEVFRKNFFNDWRKEMAVEEREVIKSLDKCDFTEIHRYFVDKAAARKVLSREEKQKLKEEAEKLQQEFGYCILDGHQEKIGNFKIEPPGLFRGRGDHPKMGMLKRRITPEDVVINCSRDSKIPEPPAGHQWKEVRSDNTVTWLAAWTESVQNSIKYIMLNPCSKLKGETAWQKFETARRLRGFVDEIRSQYRADWKSREMKTRQRAVALYFIDKLALRAGNEKEDGEAADTVGCCSLRVEHVQLHPEADGCQHVVEFDFLGKDCIRYYNRVPVEKPVYKNLQLFMENKDPRDDLFDRLTTTSLNKHLQELMDGLTAKVFRTYNASITLQEQLRALTRAEDSIAAKILSYNRANRVVAILCNHQRATPSTFEKSMQNLQTKIQAKKEQVAEARAELRRARAEHKAQGDGKSRSVLEKKRRLLEKLQEQLAQLSVQATDKEENKQVALGTSKLNYLDPRISIAWCKRFRVPVEKIYSKTQRERFAWALAMAGEDFEF
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q969P6 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S31 | Phosphorylation | Uniprot | |
| K46 | Acetylation | Uniprot | |
| K52 | Acetylation | Uniprot | |
| Y96 | Phosphorylation | Uniprot | |
| Y104 | Phosphorylation | Uniprot | |
| K112 | Acetylation | Uniprot | |
| K210 | Methylation | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| Y262 | Phosphorylation | Uniprot | |
| T282 | Phosphorylation | Uniprot | |
| Y316 | Phosphorylation | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| Y384 | Phosphorylation | Uniprot | |
| T405 | Phosphorylation | Uniprot | |
| T485 | Phosphorylation | Uniprot | |
| K515 | Acetylation | Uniprot | |
| Y559 | Phosphorylation | Uniprot |
Research Backgrounds
Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Mitochondrion.
Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.
Belongs to the type IB topoisomerase family.
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