DOCK7 Antibody - #DF9433

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Product: DOCK7 Antibody
Catalog: DF9433
Description: Rabbit polyclonal antibody to DOCK7
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 242kDa; 243kD(Calculated).
Uniprot: Q96N67
RRID: AB_2842629

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(91%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DOCK7 Antibody detects endogenous levels of total DOCK7.
RRID:
AB_2842629
Cite Format: Affinity Biosciences Cat# DF9433, RRID:AB_2842629.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3110056M06Rik; dedicator of cytokinesis 7; Dedicator of cytokinesis protein 7; Gm430; KIAA1771; MGC189434; mKIAA1771; RP23 329P19.2; ZIR2;

Immunogens

Immunogen:
Uniprot:

Q96N67(DOCK7_HUMAN) >>Visit HPA database.

Gene(ID):
DOCK7(85440)
Expression:
Q96N67 DOCK7_HUMAN:

Widely expressed.

Sequence:
MAERRAFAQKISRTVAAEVRKQISGQYSGSPQLLKNLNIVGNISHHTTVPLTEAVDPVDLEDYLITHPLAVDSGPLRDLIEFPPDDIEVVYSPRDCRTLVSAVPEESEMDPHVRDCIRSYTEDWAIVIRKYHKLGTGFNPNTLDKQKERQKGLPKQVFESDEAPDGNSYQDDQDDLKRRSMSIDDTPRGSWACSIFDLKNSLPDALLPNLLDRTPNEEIDRQNDDQRKSNRHKELFALHPSPDEEEPIERLSVPDIPKEHFGQRLLVKCLSLKFEIEIEPIFASLALYDVKEKKKISENFYFDLNSEQMKGLLRPHVPPAAITTLARSAIFSITYPSQDVFLVIKLEKVLQQGDIGECAEPYMIFKEADATKNKEKLEKLKSQADQFCQRLGKYRMPFAWTAIHLMNIVSSAGSLERDSTEVEISTGERKGSWSERRNSSIVGRRSLERTTSGDDACNLTSFRPATLTVTNFFKQEGDRLSDEDLYKFLADMRRPSSVLRRLRPITAQLKIDISPAPENPHYCLTPELLQVKLYPDSRVRPTREILEFPARDVYVPNTTYRNLLYIYPQSLNFANRQGSARNITVKVQFMYGEDPSNAMPVIFGKSSCSEFSKEAYTAVVYHNRSPDFHEEIKVKLPATLTDHHHLLFTFYHVSCQQKQNTPLETPVGYTWIPMLQNGRLKTGQFCLPVSLEKPPQAYSVLSPEVPLPGMKWVDNHKGVFNVEVVAVSSIHTQDPYLDKFFALVNALDEHLFPVRIGDMRIMENNLENELKSSISALNSSQLEPVVRFLHLLLDKLILLVIRPPVIAGQIVNLGQASFEAMASIINRLHKNLEGNHDQHGRNSLLASYIHYVFRLPNTYPNSSSPGPGGLGGSVHYATMARSAVRPASLNLNRSRSLSNSNPDISGTPTSPDDEVRSIIGSKGLDRSNSWVNTGGPKAAPWGSNPSPSAESTQAMDRSCNRMSSHTETSSFLQTLTGRLPTKKLFHEELALQWVVCSGSVRESALQQAWFFFELMVKSMVHHLYFNDKLEAPRKSRFPERFMDDIAALVSTIASDIVSRFQKDTEMVERLNTSLAFFLNDLLSVMDRGFVFSLIKSCYKQVSSKLYSLPNPSVLVSLRLDFLRIICSHEHYVTLNLPCSLLTPPASPSPSVSSATSQSSGFSTNVQDQKIANMFELSVPFRQQHYLAGLVLTELAVILDPDAEGLFGLHKKVINMVHNLLSSHDSDPRYSDPQIKARVAMLYLPLIGIIMETVPQLYDFTETHNQRGRPICIATDDYESESGSMISQTVAMAIAGTSVPQLTRPGSFLLTSTSGRQHTTFSAESSRSLLICLLWVLKNADETVLQKWFTDLSVLQLNRLLDLLYLCVSCFEYKGKKVFERMNSLTFKKSKDMRAKLEEAILGSIGARQEMVRRSRGQLGTYTIASPPERSPSGSAFGSQENLRWRKDMTHWRQNTEKLDKSRAEIEHEALIDGNLATEANLIILDTLEIVVQTVSVTESKESILGGVLKVLLHSMACNQSAVYLQHCFATQRALVSKFPELLFEEETEQCADLCLRLLRHCSSSIGTIRSHASASLYLLMRQNFEIGNNFARVKMQVTMSLSSLVGTSQNFNEEFLRRSLKTILTYAEEDLELRETTFPDQVQDLVFNLHMILSDTVKMKEHQEDPEMLIDLMYRIAKGYQTSPDLRLTWLQNMAGKHSERSNHAEAAQCLVHSAALVAEYLSMLEDRKYLPVGCVTFQNISSNVLEESAVSDDVVSPDEEGICSGKYFTESGLVGLLEQAAASFSMAGMYEAVNEVYKVLIPIHEANRDAKKLSTIHGKLQEAFSKIVHQSTGWERMFGTYFRVGFYGTKFGDLDEQEFVYKEPAITKLAEISHRLEGFYGERFGEDVVEVIKDSNPVDKCKLDPNKAYIQITYVEPYFDTYEMKDRITYFDKNYNLRRFMYCTPFTLDGRAHGELHEQFKRKTILTTSHAFPYIKTRVNVTHKEEIILTPIEVAIEDMQKKTQELAFATHQDPADPKMLQMVLQGSVGTTVNQGPLEVAQVFLSEIPSDPKLFRHHNKLRLCFKDFTKRCEDALRKNKSLIGPDQKEYQRELERNYHRLKEALQPLINRKIPQLYKAVLPVTCHRDSFSRMSLRKMDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
100
Sheep
91
Zebrafish
73
Horse
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96N67 As Substrate

Site PTM Type Enzyme
S24 Phosphorylation
Y27 Phosphorylation
S28 Phosphorylation
S30 Phosphorylation
K133 Ubiquitination
T136 Phosphorylation
T142 Phosphorylation
K155 Ubiquitination
S168 Phosphorylation
Y169 Phosphorylation
K177 Ubiquitination
S180 Phosphorylation
S182 Phosphorylation
T186 Phosphorylation
S190 Phosphorylation
K199 Ubiquitination
T214 Phosphorylation
K233 Ubiquitination
K258 Ubiquitination
K366 Ubiquitination
K381 Methylation
K381 Ubiquitination
S414 Phosphorylation
S432 Phosphorylation
S439 Phosphorylation
S440 Phosphorylation
T450 Phosphorylation
T451 Phosphorylation
S452 Phosphorylation
S497 Phosphorylation
Y522 Phosphorylation
K532 Ubiquitination
T649 Phosphorylation
S864 Phosphorylation
S873 Phosphorylation
Y876 Phosphorylation
T878 Phosphorylation
S882 Phosphorylation
S888 Phosphorylation
S894 Phosphorylation
S896 Phosphorylation
S898 Phosphorylation
S900 Phosphorylation
S905 Phosphorylation
T907 Phosphorylation
T909 Phosphorylation
S910 Phosphorylation
S917 Phosphorylation
S927 Phosphorylation
S929 Phosphorylation
S946 Phosphorylation
S948 Phosphorylation
S958 Phosphorylation
S963 Phosphorylation
S964 Phosphorylation
S969 Phosphorylation
T981 Phosphorylation
Y1242 Phosphorylation
Y1257 Phosphorylation P04626 (ERBB2)
T1296 Phosphorylation
S1297 Phosphorylation
T1302 Phosphorylation
S1306 Phosphorylation
Y1372 Phosphorylation
S1383 Phosphorylation
T1385 Phosphorylation
K1388 Methylation
S1403 Phosphorylation
R1415 Methylation
Y1421 Phosphorylation
T1422 Phosphorylation
S1425 Phosphorylation
S1430 Phosphorylation
S1432 Phosphorylation
S1434 Phosphorylation
S1438 Phosphorylation
K1446 Ubiquitination
T1455 Phosphorylation
S1874 Phosphorylation
K1962 Acetylation
K1962 Methylation
T1991 Phosphorylation
K2003 Ubiquitination
K2080 Ubiquitination
K2088 Ubiquitination
K2112 Ubiquitination
Y2117 Phosphorylation
S2129 Phosphorylation
S2131 Phosphorylation
S2134 Phosphorylation

Research Backgrounds

Function:

Functions as a guanine nucleotide exchange factor (GEF), which activates Rac1 and Rac3 Rho small GTPases by exchanging bound GDP for free GTP. Does not have a GEF activity for CDC42. Required for STMN1 'Ser-15' phosphorylation during axon formation and consequently for neuronal polarization. As part of the DISP complex, may regulate the association of septins with actin and thereby regulate the actin cytoskeleton. Has a role in pigmentation (By similarity). Involved in the regulation of cortical neurogenesis through the control of radial glial cells (RGCs) proliferation versus differentiation; negatively regulates the basal-to-apical interkinetic nuclear migration of RGCs by antagonizing the microtubule growth-promoting function of TACC3 (By similarity).

Subcellular Location:

Cell projection>Axon.
Note: Enriched in the developing axons of hippocampal neurons.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Component of the DOCK7-induced septin displacement/DISP complex, at least composed of DOCK7, LRCH3 and MYO6. Interacts with TSC1. Interacts with nucleotide-free RAC1 and RAC3. Interacts with TACC3 and CRY1 (By similarity). Interacts with NOD2.

Family&Domains:

The DHR-2 domain mediates GEF activity.

Belongs to the DOCK family.

Restrictive clause

 

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