DOCK11 Antibody - #DF9432

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Product: DOCK11 Antibody
Catalog: DF9432
Description: Rabbit polyclonal antibody to DOCK11
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 238kDa; 238kD(Calculated).
Uniprot: Q5JSL3
RRID: AB_2842628

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
DOCK11 Antibody detects endogenous levels of total DOCK11.
RRID:
AB_2842628
Cite Format: Affinity Biosciences Cat# DF9432, RRID:AB_2842628.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACG; Activated Cdc42-associated guanine nucleotide exchange factor; Dedicator of cytokinesis protein 11; DOC11_HUMAN; Dock11; ZIZ2; Zizimin-2;

Immunogens

Immunogen:
Uniprot:

Q5JSL3(DOC11_HUMAN) >>Visit HPA database.

Gene(ID):
DOCK11(139818)
Sequence:
MAEVRKFTKRLSKPGTAAELRQSVSEAVRGSVVLEKAKVVEPLDYENVIAQRKTQIYSDPLRDLLMFPMEDISISVIGRQRRTVQSTVPEDAEKRAQSLFVKECIKTYSTDWHVVNYKYEDFSGDFRMLPCKSLRPEKIPNHVFEIDEDCEKDEDSSSLCSQKGGVIKQGWLHKANVNSTITVTMKVFKRRYFYLTQLPDGSYILNSYKDEKNSKESKGCIYLDACIDVVQCPKMRRHAFELKMLDKYSHYLAAETEQEMEEWLITLKKIIQINTDSLVQEKKETVETAQDDETSSQGKAENIMASLERSMHPELMKYGRETEQLNKLSRGDGRQNLFSFDSEVQRLDFSGIEPDIKPFEEKCNKRFLVNCHDLTFNILGQIGDNAKGPPTNVEPFFINLALFDVKNNCKISADFHVDLNPPSVREMLWGSSTQLASDGSPKGSSPESYIHGIAESQLRYIQQGIFSVTNPHPEIFLVARIEKVLQGNITHCAEPYIKNSDPVKTAQKVHRTAKQVCSRLGQYRMPFAWAARPIFKDTQGSLDLDGRFSPLYKQDSSKLSSEDILKLLSEYKKPEKTKLQIIPGQLNITVECVPVDLSNCITSSYVPLKPFEKNCQNITVEVEEFVPEMTKYCYPFTIYKNHLYVYPLQLKYDSQKTFAKARNIAVCVEFRDSDESDASALKCIYGKPAGSVFTTNAYAVVSHHNQNPEFYDEIKIELPIHLHQKHHLLFTFYHVSCEINTKGTTKKQDTVETPVGFAWVPLLKDGRIITFEQQLPVSANLPPGYLNLNDAESRRQCNVDIKWVDGAKPLLKIKSHLESTIYTQDLHVHKFFHHCQLIQSGSKEVPGELIKYLKCLHAMEIQVMIQFLPVILMQLFRVLTNMTHEDDVPINCTMVLLHIVSKCHEEGLDSYLRSFIKYSFRPEKPSAPQAQLIHETLATTMIAILKQSADFLSINKLLKYSWFFFEIIAKSMATYLLEENKIKLPRGQRFPETYHHVLHSLLLAIIPHVTIRYAEIPDESRNVNYSLASFLKRCLTLMDRGFIFNLINDYISGFSPKDPKVLAEYKFEFLQTICNHEHYIPLNLPMAFAKPKLQRVQDSNLEYSLSDEYCKHHFLVGLLLRETSIALQDNYEIRYTAISVIKNLLIKHAFDTRYQHKNQQAKIAQLYLPFVGLLLENIQRLAGRDTLYSCAAMPNSASRDEFPCGFTSPANRGSLSTDKDTAYGSFQNGHGIKREDSRGSLIPEGATGFPDQGNTGENTRQSSTRSSVSQYNRLDQYEIRSLLMCYLYIVKMISEDTLLTYWNKVSPQELINILILLEVCLFHFRYMGKRNIARVHDAWLSKHFGIDRKSQTMPALRNRSGVMQARLQHLSSLESSFTLNHSSTTTEADIFHQALLEGNTATEVSLTVLDTISFFTQCFKTQLLNNDGHNPLMKKVFDIHLAFLKNGQSEVSLKHVFASLRAFISKFPSAFFKGRVNMCAAFCYEVLKCCTSKISSTRNEASALLYLLMRNNFEYTKRKTFLRTHLQIIIAVSQLIADVALSGGSRFQESLFIINNFANSDRPMKATAFPAEVKDLTKRIRTVLMATAQMKEHEKDPEMLIDLQYSLAKSYASTPELRKTWLDSMAKIHVKNGDFSEAAMCYVHVAALVAEFLHRKKLFPNGCSAFKKITPNIDEEGAMKEDAGMMDVHYSEEVLLELLEQCVDGLWKAERYEIISEISKLIVPIYEKRREFEKLTQVYRTLHGAYTKILEVMHTKKRLLGTFFRVAFYGQSFFEEEDGKEYIYKEPKLTGLSEISLRLVKLYGEKFGTENVKIIQDSDKVNAKELDPKYAHIQVTYVKPYFDDKELTERKTEFERNHNISRFVFEAPYTLSGKKQGCIEEQCKRRTILTTSNSFPYVKKRIPINCEQQINLKPIDVATDEIKDKTAELQKLCSSTDVDMIQLQLKLQGCVSVQVNAGPLAYARAFLNDSQASKYPPKKVSELKDMFRKFIQACSIALELNERLIKEDQVEYHEGLKSNFRDMVKELSDIIHEQILQEDTMHSPWMSNTLHVFCAISGTSSDRGYGSPRYAEV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Zebrafish
86
Horse
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5JSL3 As Substrate

Site PTM Type Enzyme
S12 Phosphorylation
T16 Phosphorylation
S23 Phosphorylation
S25 Phosphorylation
S31 Phosphorylation
Y57 Phosphorylation
S58 Phosphorylation
T83 Phosphorylation
S86 Phosphorylation
T87 Phosphorylation
S98 Phosphorylation
S123 Phosphorylation
K132 Ubiquitination
K138 Ubiquitination
S158 Phosphorylation
S161 Phosphorylation
K163 Ubiquitination
Y248 Phosphorylation
S249 Phosphorylation
Y251 Phosphorylation
T266 Phosphorylation
T275 Phosphorylation
S277 Phosphorylation
T294 Phosphorylation
S296 Phosphorylation
S306 Phosphorylation
T322 Phosphorylation
S329 Phosphorylation
S412 Phosphorylation
S437 Phosphorylation
S440 Phosphorylation
K498 Ubiquitination
S500 Phosphorylation
T505 Phosphorylation
K553 Ubiquitination
S560 Phosphorylation
Y571 Phosphorylation
K631 Acetylation
K656 Ubiquitination
T731 Phosphorylation
T1010 Phosphorylation
S1029 Phosphorylation
K1147 Ubiquitination
Y1188 Phosphorylation
S1208 Phosphorylation
T1221 Phosphorylation
Y1223 Phosphorylation
S1225 Phosphorylation
S1237 Phosphorylation
S1240 Phosphorylation
T1247 Phosphorylation
S1267 Phosphorylation
S1269 Phosphorylation
Y1271 Phosphorylation
Y1277 Phosphorylation
Y1288 Phosphorylation
S1350 Phosphorylation
T1352 Phosphorylation
T1378 Phosphorylation
T1620 Phosphorylation
S1624 Phosphorylation
S1636 Phosphorylation
Y1642 Phosphorylation
T1790 Phosphorylation
T1809 Phosphorylation
K2017 Ubiquitination

Research Backgrounds

Function:

Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Required for marginal zone (MZ) B-cell development, is associated with early bone marrow B-cell development, MZ B-cell formation, MZ B-cell number and marginal metallophilic macrophages morphology. Facilitates filopodia formation through the activation of CDC42.

Subunit Structure:

Interacts with CDC42.

Family&Domains:

The DHR-2 domain is necessary for the GEF activity. The DHR-2 domain mediates interaction with activated CDC42 in conjunction with residues 66-126.

Belongs to the DOCK family.

Restrictive clause

 

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