NCAPD3 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from B16F10 cells, using NCAPD3 Antibody.

Product:	NCAPD3 Antibody
Catalog:	DF9413
Description:	Rabbit polyclonal antibody to NCAPD3
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	169kDa; 169kD(Calculated).
Uniprot:	P42695
RRID:	AB_2842609

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

NCAPD3 Antibody detects endogenous levels of total NCAPD3.

RRID:

AB_2842609
Cite Format: Affinity Biosciences Cat# DF9413, RRID:AB_2842609.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CAP D3; CAPD 3; CAPD3; CNDD3_HUMAN; Condensin II complex subunit D3; Condensin-2 complex subunit D3; FLJ42888; hCAP-D3; Hcp 6; Hcp6; hHCP 6; hHCP6; KIAA0056; MGC104671; NCAPD 3; Ncapd3; Non SMC condensin II complex subunit D3; Non-SMC condensin II complex subunit D3; OTTHUMP00000235403; OTTHUMP00000235404;

Immunogens

Immunogen:

Uniprot:

P42695(CNDD3_HUMAN) >>Visit HPA database.

Gene(ID):

NCAPD3(23310)

Sequence:

P42695 CNDD3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVALRGLGSGLQPWCPLDLRLEWVDTVWELDFTETEPLDPSIEAEIIETGLAAFTKLYESLLPFATGEHGSMESIWTFFIENNVSHSTLVALFYHFVQIVHKKNVSVQYREYGLHAAGLYFLLLEVPGSVANQVFHPVMFDKCIQTLKKSWPQESNLNRKRKKEQPKSSQANPGRHRKRGKPPRREDIEMDEIIEEQEDENICFSARDLSQIRNAIFHLLKNFLRLLPKFSLKEKPQCVQNCIEVFVSLTNFEPVLHECHVTQARALNQAKYIPELAYYGLYLLCSPIHGEGDKVISCVFHQMLSVILMLEVGEGSHRAPLAVTSQVINCRNQAVQFISALVDELKESIFPVVRILLQHICAKVVDKSEYRTFAAQSLVQLLSKLPCGEYAMFIAWLYKYSRSSKIPHRVFTLDVVLALLELPEREVDNTLSLEHQKFLKHKFLVQEIMFDRCLDKAPTVRSKALSSFAHCLELTVTSASESILELLINSPTFSVIESHPGTLLRNSSAFSYQRQTSNRSEPSGEINIDSSGETVGSGERCVMAMLRRRIRDEKTNVRKSALQVLVSILKHCDVSGMKEDLWILQDQCRDPAVSVRKQALQSLTELLMAQPRCVQIQKAWLRGVVPVVMDCESTVQEKALEFLDQLLLQNIRHHSHFHSGDDSQVLAWALLTLLTTESQELSRYLNKAFHIWSKKEKFSPTFINNVISHTGTEHSAPAWMLLSKIAGSSPRLDYSRIIQSWEKISSQQNPNSNTLGHILCVIGHIAKHLPKSTRDKVTDAVKCKLNGFQWSLEVISSAVDALQRLCRASAETPAEEQELLTQVCGDVLSTCEHRLSNIVLKENGTGNMDEDLLVKYIFTLGDIAQLCPARVEKRIFLLIQSVLASSADADHSPSSQGSSEAPASQPPPQVRGSVMPSVIRAHAIITLGKLCLQHEDLAKKSIPALVRELEVCEDVAVRNNVIIVMCDLCIRYTIMVDKYIPNISMCLKDSDPFIRKQTLILLTNLLQEEFVKWKGSLFFRFVSTLIDSHPDIASFGEFCLAHLLLKRNPVMFFQHFIECIFHFNNYEKHEKYNKFPQSEREKRLFSLKGKSNKERRMKIYKFLLEHFTDEQRFNITSKICLSILACFADGILPLDLDASELLSDTFEVLSSKEIKLLAMRSKPDKDLLMEEDDMALANVVMQEAQKKLISQVQKRNFIENIIPIIISLKTVLEKNKIPALRELMHYLREVMQDYRDELKDFFAVDKQLASELEYDMKKYQEQLVQEQELAKHADVAGTAGGAEVAPVAQVALCLETVPVPAGQENPAMSPAVSQPCTPRASAGHVAVSSPTPETGPLQRLLPKARPMSLSTIAILNSVKKAVESKSRHRSRSLGVLPFTLNSGSPEKTCSQVSSYSLEQESNGEIEHVTKRAISTPEKSISDVTFGAGVSYIGTPRTPSSAKEKIEGRSQGNDILCLSLPDKPPPQPQQWNVRSPARNKDTPACSRRSLRKTPLKTAN

PTMs - P42695 As Substrate

P42695

Site	PTM Type	Source
S106	Phosphorylation	Uniprot
Y109	Phosphorylation	Uniprot
K148	Ubiquitination	Uniprot
K149	Ubiquitination	Uniprot
K167	Ubiquitination	Uniprot
K229	Ubiquitination	Uniprot
S325	Phosphorylation	Uniprot
T412	Phosphorylation	Uniprot
T430	Phosphorylation	Uniprot
K442	Ubiquitination	Uniprot
K456	Ubiquitination	Uniprot
S507	Phosphorylation	Uniprot
S508	Phosphorylation	Uniprot
S511	Phosphorylation	Uniprot
Y512	Phosphorylation	Uniprot
T516	Phosphorylation	Uniprot
S517	Phosphorylation	Uniprot
S530	Phosphorylation	Uniprot
S531	Phosphorylation	Uniprot
T534	Phosphorylation	Uniprot
K554	Ubiquitination	Uniprot
K559	Ubiquitination	Uniprot
S567	Phosphorylation	Uniprot
K578	Ubiquitination	Uniprot
K597	Ubiquitination	Uniprot
K618	Ubiquitination	Uniprot
K687	Ubiquitination	Uniprot
K697	Ubiquitination	Uniprot
K724	Ubiquitination	Uniprot
S728	Phosphorylation	Uniprot
K743	Ubiquitination	Uniprot
K771	Ubiquitination	Uniprot
K776	Ubiquitination	Uniprot
K782	Ubiquitination	Uniprot
K841	Ubiquitination	Uniprot
S895	Phosphorylation	Uniprot
K929	Ubiquitination	Uniprot
K939	Ubiquitination	Uniprot
K940	Ubiquitination	Uniprot
Y972	Phosphorylation	Uniprot
K988	Ubiquitination	Uniprot
K1074	Ubiquitination	Uniprot
K1155	Ubiquitination	Uniprot
K1187	Ubiquitination	Uniprot
K1194	Ubiquitination	Uniprot
K1216	Acetylation	Uniprot
K1246	Ubiquitination	Uniprot
S1250	Phosphorylation	Uniprot
K1257	Ubiquitination	Uniprot
K1258	Ubiquitination	Uniprot
S1309	Phosphorylation	Uniprot
S1313	Phosphorylation	Uniprot
T1317	Phosphorylation	Uniprot
S1321	Phosphorylation	Uniprot
S1328	Phosphorylation	Uniprot
S1329	Phosphorylation	Uniprot
T1331	Phosphorylation	Uniprot
T1334	Phosphorylation	Uniprot
S1348	Phosphorylation	Uniprot
S1350	Phosphorylation	Uniprot
T1351	Phosphorylation	Uniprot
S1357	Phosphorylation	Uniprot
S1366	Phosphorylation	Uniprot
S1370	Phosphorylation	Uniprot
S1372	Phosphorylation	Uniprot
T1379	Phosphorylation	Uniprot
S1382	Phosphorylation	Uniprot
S1384	Phosphorylation	Uniprot
S1393	Phosphorylation	Uniprot
K1410	Ubiquitination	Uniprot
S1414	Phosphorylation	Uniprot
T1415	Phosphorylation	Uniprot
S1419	Phosphorylation	Uniprot
T1424	Phosphorylation	Uniprot
T1434	Phosphorylation	Uniprot
T1437	Phosphorylation	Uniprot
S1449	Phosphorylation	Uniprot
S1458	Phosphorylation	Uniprot
K1462	Ubiquitination	Uniprot
S1474	Phosphorylation	Uniprot
S1488	Phosphorylation	Uniprot
T1492	Phosphorylation	Uniprot
T1496	Phosphorylation	Uniprot

Research Backgrounds

P42695_CNDD3_HUMAN

Function:

Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis. May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Specifically required for decatenation of centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of the condensin-2 complex, which contains the SMC2 and SMC4 heterodimer, and 3 non SMC subunits that probably regulate the complex: NCAPH2, NCAPD3 and NCAPG2. Interacts with BRD4 (isoform B), leading to insulate chromatin from DNA damage response pathway.

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For Research Use Only.
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NCAPD3 Antibody - #DF9413