CCDC8 Antibody - #DF9405
| Product: | CCDC8 Antibody |
| Catalog: | DF9405 |
| Description: | Rabbit polyclonal antibody to CCDC8 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep |
| Mol.Wt.: | 59 kDa; 59kD(Calculated). |
| Uniprot: | Q9H0W5 |
| RRID: | AB_2842601 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9405, RRID:AB_2842601.
Fold/Unfold
CCDC8; CCDC8_HUMAN; Coiled-coil domain-containing protein 8; DKFZp564K0322; ENSMUSG00000041117; MGC72567;
Immunogens
Widely expressed with low levels in spleen, skeletal muscle, small intestine, kidney and liver.
- Q9H0W5 CCDC8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLQIGEDVDYLLIPREVRLAGGVWRVISKPATKEAEFRERLTQFLEEEGRTLEDVARIMEKSTPHPPQPPKKPKEPRVRRRVQQMVTPPPRLVVGTYDSSNASDSEFSDFETSRDKSRQGPRRGKKVRKMPVSYLGSKFLGSDLESEDDEELVEAFLRRQEKQPSAPPARRRVNLPVPMFEDNLGPQLSKADRWREYVSQVSWGKLKRRVKGWAPRAGPGVGEARLASTAVESAGVSSAPEGTSPGDRLGNAGDVCVPQASPRRWRPKINWASFRRRRKEQTAPTGQGADIEADQGGEAADSQREEAIADQREGAAGNQRAGAPADQGAEAADNQREEAADNQRAGAPAEEGAEAADNQREEAADNQRAEAPADQRSQGTDNHREEAADNQRAEAPADQGSEVTDNQREEAVHDQRERAPAVQGADNQRAQARAGQRAEAAHNQRAGAPGIQEAEVSAAQGTTGTAPGARARKQVKTVRFQTPGRFSWFCKRRRAFWHTPRLPTLPKRVPRAGEARNLRVLRAEARAEAEQGEQEDQL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H0W5 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T87 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| Y197 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| R320 | Methylation | Uniprot | |
| R344 | Methylation | Uniprot | |
| R445 | Methylation | Uniprot | |
| T477 | Phosphorylation | Uniprot | |
| S487 | Phosphorylation | Uniprot | |
| T499 | Phosphorylation | Uniprot |
Research Backgrounds
Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer. Required for localization of CUL7 to the centrosome.
Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Widely expressed with low levels in spleen, skeletal muscle, small intestine, kidney and liver.
Component of the 3M complex, composed of core components CUL7, CCDC8 and OBSL1. Interacts (via PxLPxI/L motif) with ANKRA2 (via ankyrin repeats); may link the 3M complex to histone deacetylases including HDAC4 and HDAC5.
The PxLPxI/L motif mediates interaction with ankyrin repeats of ANKRA2.
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