DENND4A Antibody - #DF9399

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Product: DENND4A Antibody
Catalog: DF9399
Description: Rabbit polyclonal antibody to DENND4A
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 209kDa; 209kD(Calculated).
Uniprot: Q7Z401
RRID: AB_2842595

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(82%), Horse(91%), Sheep(91%), Rabbit(82%), Dog(91%)
Clonality:
Polyclonal
Specificity:
DENND4A Antibody detects endogenous levels of total DENND4A.
RRID:
AB_2842595
Cite Format: Affinity Biosciences Cat# DF9399, RRID:AB_2842595.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C-myc promoter-binding protein; DENN domain-containing protein 4A; DENN/MADD domain containing 4A; DENND4A; FLJ33949; IRLB; KIAA0476; MYCPBP; MYCPP_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q7Z401(MYCPP_HUMAN) >>Visit HPA database.

Gene(ID):
DENND4A(10260)
Expression:
Q7Z401 MYCPP_HUMAN:

Expressed ubiquitously. Highest expression in bone marrow, medium in peripheral blood lymphocytes and lowest in spleen. In brain, breast, and prostate, higher expression was seen in normal cells than in tumor cells. Expression is regulated in a growth- and cell cycle-dependent manner.

Sequence:
MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Sheep
91
Dog
91
Bovine
82
Rabbit
82
Chicken
55
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z401 As Substrate

Site PTM Type Enzyme
K178 Ubiquitination
S186 Phosphorylation
S190 Phosphorylation
S199 Phosphorylation
K202 Ubiquitination
S207 Phosphorylation
Y208 Phosphorylation
K287 Ubiquitination
T295 Phosphorylation
S296 Phosphorylation
T519 Phosphorylation
Y547 Phosphorylation
K718 Ubiquitination
S728 Phosphorylation
S731 Phosphorylation
Y823 Phosphorylation
T882 Phosphorylation
S900 Phosphorylation
S910 Phosphorylation
T921 Phosphorylation
S922 Phosphorylation
T923 Phosphorylation
S935 Phosphorylation
S939 Phosphorylation
S941 Phosphorylation
S943 Phosphorylation
Y958 Phosphorylation
S963 Phosphorylation
K977 Ubiquitination
S1015 Phosphorylation
T1019 Phosphorylation
S1035 Phosphorylation
S1093 Phosphorylation
S1098 Phosphorylation
S1099 Phosphorylation
S1117 Phosphorylation
S1128 Phosphorylation
T1130 Phosphorylation
S1151 Phosphorylation
S1152 Phosphorylation
S1154 Phosphorylation
S1192 Phosphorylation
S1194 Phosphorylation
T1197 Phosphorylation
S1217 Phosphorylation
T1223 Phosphorylation
S1225 Phosphorylation
S1240 Phosphorylation
S1251 Phosphorylation
S1256 Phosphorylation
S1281 Phosphorylation
S1282 Phosphorylation
S1286 Phosphorylation
T1288 Phosphorylation
S1295 Phosphorylation
S1305 Phosphorylation
K1315 Ubiquitination
K1328 Ubiquitination
K1336 Acetylation
Y1338 Phosphorylation
Y1344 Phosphorylation
K1349 Ubiquitination
S1404 Phosphorylation
S1410 Phosphorylation
Y1504 Phosphorylation
S1508 Phosphorylation
S1511 Phosphorylation
T1512 Phosphorylation
K1555 Ubiquitination
S1564 Phosphorylation
S1572 Phosphorylation
R1586 Methylation
S1587 Phosphorylation
S1589 Phosphorylation
T1590 Phosphorylation
S1591 Phosphorylation
K1765 Ubiquitination

Research Backgrounds

Function:

Probable guanine nucleotide exchange factor (GEF) which may activate RAB10. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. According to it may bind to ISRE-like element (interferon-stimulated response element) of MYC P2 promoter.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed ubiquitously. Highest expression in bone marrow, medium in peripheral blood lymphocytes and lowest in spleen. In brain, breast, and prostate, higher expression was seen in normal cells than in tumor cells. Expression is regulated in a growth- and cell cycle-dependent manner.

Restrictive clause

 

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For Research Use Only.
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