CLASP2 Antibody - #DF9398

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Product: CLASP2 Antibody
Catalog: DF9398
Description: Rabbit polyclonal antibody to CLASP2
Application: WB
Reactivity: Human, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 141kDa; 141kD(Calculated).
Uniprot: O75122
RRID: AB_2842594

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Prediction:
Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CLASP2 Antibody detects endogenous levels of total CLASP2.
RRID:
AB_2842594
Cite Format: Affinity Biosciences Cat# DF9398, RRID:AB_2842594.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CLAP2_HUMAN; Clasp2; CLIP associated protein 2; CLIP associating protein 2; CLIP-associating protein 2; Cytoplasmic linker associated protein 2; Cytoplasmic linker-associated protein 2; hOrbit2; KIAA0627; Multiple asters (Mast) like homolog 2; Protein Orbit homolog 2;

Immunogens

Immunogen:
Uniprot:

O75122(CLAP2_HUMAN) >>Visit HPA database.

Gene(ID):
CLASP2(23122)
Expression:
O75122 CLAP2_HUMAN:

Brain-specific.

Sequence:
MAMGDDKSFDDEESVDGNRPSSAASAFKVPAPKTSGNPANSARKPGSAGGPKVGGASKEGGAGAVDEDDFIKAFTDVPSIQIYSSRELEETLNKIREILSDDKHDWDQRANALKKIRSLLVAGAAQYDCFFQHLRLLDGALKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVPTLFNLVPNSAKVMATSGCAAIRFIIRHTHVPRLIPLITSNCTSKSVPVRRRSFEFLDLLLQEWQTHSLERHAAVLVETIKKGIHDADAEARVEARKTYMGLRNHFPGEAETLYNSLEPSYQKSLQTYLKSSGSVASLPQSDRSSSSSQESLNRPFSSKWSTANPSTVAGRVSAGSSKASSLPGSLQRSRSDIDVNAAAGAKAHHAAGQSVRSGRLGAGALNAGSYASLEDTSDKLDGTASEDGRVRAKLSAPLAGMGNAKADSRGRSRTKMVSQSQPGSRSGSPGRVLTTTALSTVSSGVQRVLVNSASAQKRSKIPRSQGCSREASPSRLSVARSSRIPRPSVSQGCSREASRESSRDTSPVRSFQPLASRHHSRSTGALYAPEVYGASGPGYGISQSSRLSSSVSAMRVLNTGSDVEEAVADALKKPARRRYESYGMHSDDDANSDASSACSERSYSSRNGSIPTYMRQTEDVAEVLNRCASSNWSERKEGLLGLQNLLKNQRTLSRVELKRLCEIFTRMFADPHGKRVFSMFLETLVDFIQVHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDVTRESFPNDLQFNILMRFTVDQTQTPSLKVKVAILKYIETLAKQMDPGDFINSSETRLAVSRVITWTTEPKSSDVRKAAQSVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLRNTGNGTQSSMGSPLTRPTPRSPANWSSPLTSPTNTSQNTLSPSAFDYDTENMNSEDIYSSLRGVTEAIQNFSFRSQEDMNEPLKRDSKKDDGDSMCGGPGMSDPRAGGDATDSSQTALDNKASLLHSMPTHSSPRSRDYNPYNYSDSISPFNKSALKEAMFDDDADQFPDDLSLDHSDLVAELLKELSNHNERVEERKIALYELMKLTQEESFSVWDEHFKTILLLLLETLGDKEPTIRALALKVLREILRHQPARFKNYAELTVMKTLEAHKDPHKEVVRSAEEAASVLATSISPEQCIKVLCPIIQTADYPINLAAIKMQTKVIERVSKETLNLLLPEIMPGLIQGYDNSESSVRKACVFCLVAVHAVIGDELKPHLSQLTGSKMKLLNLYIKRAQTGSGGADPTTDVSGQS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
92
Sheep
92
Chicken
67
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75122 As Substrate

Site PTM Type Enzyme
K7 Acetylation
K7 Ubiquitination
S8 Phosphorylation
S14 Phosphorylation
S21 Phosphorylation
S25 Phosphorylation
K28 Ubiquitination
S41 Phosphorylation
S47 Phosphorylation
S57 Phosphorylation
T91 Phosphorylation
K94 Ubiquitination
S100 Phosphorylation
T181 Phosphorylation
S189 Phosphorylation
S299 Phosphorylation
Y300 Phosphorylation
S310 Phosphorylation
S313 Phosphorylation
S316 Phosphorylation
R322 Methylation
S323 Phosphorylation
S325 Phosphorylation
S326 Phosphorylation
S327 Phosphorylation
S330 Phosphorylation
S352 Phosphorylation
S355 Phosphorylation
S359 Phosphorylation
S360 Phosphorylation
S364 Phosphorylation
S368 Phosphorylation
S370 Phosphorylation
R391 Methylation
S404 Phosphorylation
S407 Phosphorylation
T418 Phosphorylation
S420 Phosphorylation
S430 Phosphorylation
S453 Phosphorylation
S455 Phosphorylation
S459 Phosphorylation
S461 Phosphorylation
S463 Phosphorylation
S477 Phosphorylation
S478 Phosphorylation
S487 Phosphorylation
S489 Phosphorylation
S494 Phosphorylation
S499 Phosphorylation P49841 (GSK3B)
S503 Phosphorylation P49841 (GSK3B)
S507 Phosphorylation P06493 (CDK1) , P49841 (GSK3B)
S509 Phosphorylation
S512 Phosphorylation
S516 Phosphorylation
S523 Phosphorylation
S525 Phosphorylation
S529 Phosphorylation
S533 Phosphorylation P49841 (GSK3B)
S536 Phosphorylation
S537 Phosphorylation P49841 (GSK3B)
S541 Phosphorylation P06493 (CDK1)
S551 Phosphorylation
S584 Phosphorylation
S585 Phosphorylation
S587 Phosphorylation
T594 Phosphorylation
S596 Phosphorylation
K607 Ubiquitination
Y614 Phosphorylation
S616 Phosphorylation
S621 Phosphorylation
S627 Phosphorylation
S630 Phosphorylation
S634 Phosphorylation
S639 Phosphorylation
S640 Phosphorylation
S644 Phosphorylation
T647 Phosphorylation
Y648 Phosphorylation
K671 Ubiquitination
R710 Methylation
T781 Phosphorylation
T787 Phosphorylation
T857 Phosphorylation
T866 Phosphorylation
T872 Phosphorylation
K873 Ubiquitination
T886 Phosphorylation
S888 Phosphorylation
S889 Phosphorylation
S892 Phosphorylation
T895 Phosphorylation
R900 Methylation
S901 Phosphorylation
S907 Phosphorylation
Y927 Phosphorylation
Y938 Phosphorylation
S952 Phosphorylation
S955 Phosphorylation
S974 Phosphorylation
C976 S-Nitrosylation
S982 Phosphorylation
T991 Phosphorylation
S993 Phosphorylation
S994 Phosphorylation
T996 Phosphorylation
K1001 Ubiquitination
T1010 Phosphorylation
S1012 Phosphorylation
S1013 Phosphorylation
S1016 Phosphorylation
Y1019 Phosphorylation
Y1022 Phosphorylation
Y1024 Phosphorylation
S1025 Phosphorylation
S1027 Phosphorylation
S1029 Phosphorylation
S1034 Phosphorylation
S1053 Phosphorylation
S1057 Phosphorylation
K1078 Methylation
Y1082 Phosphorylation
T1088 Phosphorylation
S1092 Phosphorylation
T1110 Phosphorylation
K1124 Ubiquitination
Y1140 Phosphorylation
K1204 Ubiquitination
Y1273 Phosphorylation
S1294 Phosphorylation

Research Backgrounds

Function:

Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.

PTMs:

Phosphorylated by GSK3B. Phosphorylation reduces MAPRE1 binding. Phosphorylation by GSK3B may negatively regulate binding to microtubule lattices in lamella.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Chromosome>Centromere>Kinetochore. Cytoplasm>Cytoskeleton>Spindle. Golgi apparatus. Golgi apparatus>trans-Golgi network. Cell membrane. Cell projection>Ruffle membrane.
Note: Localizes to microtubule plus ends (PubMed:15631994). Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase (PubMed:16866869, PubMed:16914514). In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2 (PubMed:16824950). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain-specific.

Subunit Structure:

Interacts with microtubules. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with CLIP2, ERC1, MAPRE3, PHLDB2 and RSN. The interaction with ERC1 may be mediated by PHLDB2. Interacts with GCC2; recruits CLASP2 to Golgi membranes. Interacts with MACF1 (By similarity).

Family&Domains:

The two SXIP sequence motifs mediate interaction with MAPRE1; this is necessary for targeting to growing microtubule plus ends.

Two TOG regions display structural characteristics similar to HEAT repeat domains and mediate interaction with microtubules.

Belongs to the CLASP family.

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