KIF4B Antibody - #DF9385
(2)
| Product: | KIF4B Antibody |
| Catalog: | DF9385 |
| Description: | Rabbit polyclonal antibody to KIF4B |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Mol.Wt.: | 140kDa; 140kD(Calculated). |
| Uniprot: | Q2VIQ3 |
| RRID: | AB_2842581 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
KIF4B Antibody detects endogenous levels of total KIF4B.
RRID:
AB_2842581
Cite Format: Affinity Biosciences Cat# DF9385, RRID:AB_2842581.
Cite Format: Affinity Biosciences Cat# DF9385, RRID:AB_2842581.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q2VIQ3 KIF4B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKEEVKGIPVRVALRCRPLVPKEISEGCQMCLSFVPGETQVVVGTDKSFTYDFVFDPCTEQEEVFNKAVAPLIKGIFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTVGIIPRVIQLLFKEIDKKSDFEFTLKVSYLEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLTEKTVLVALDTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISIEQRKKSDKNCSFRSKLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGDDKKGSFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLSTLRYADRARKIKNKPIVNIDPHTAELNHLKQQVQQLQVLLLQAHGGTLPGSINAEPSENLQSLMEKNQSLVEENEKLSRCLSKAAGQTAQMLERIILTEQVNEKLNAKLEELRQHVACKLDLQKLVETLEDQELKENVEIICNLQQLITQLSDETVACTAAAIDTAVEEEAQVETSPETSRSSDAFTTQHALHQAQMSKEVVELNNALALKEALVRKMTQNDNQLQPIQFQYQDNIKNLELEVINLQKEKEELVRELQTAKKNVNQAKLSEHRHKLLQELEGQIADLKKKLNEQSKLLKLKESTERTVSKLNQEIWMMKNQRVQLMRQMKEDAEKFRQWKQKKDKEVIQLKERDRKRQYELLKLERNFQKQSSVLRRKTEEAAAANKRLKDALQKQREVTDKRKETQSHGKEGIAARVRNWLGNEIEVMVSTEEAKRHLNDLLEDRKILAQDVVQLKEKKESRENPPPKLRKCTFSLSEVHGQVLESEDCITKQIESLETEMELRSAQIADLQQKLLDAESEDRPKQCWENIATILEAKCALKYLIGELVSSKIHVTKLENSLRQSKASCADMQKMLFEEQNHFSEIETELQAELVRMEQQHQEKVLYLVSQLQESQMAEKQLEKSASEKEQQLVSTLQCQDEELEKMREVCEQNQQLLQENEIIKQKLILLQVASRQKHLPNDTLLSPDSSFEYIPPKPKPSRVKEKFLEQSMDIEDLKYCSEHSVNEHEDGDGDGDSDEGDDEEWKPTKLVKVSRKNIQGCSCKGWCGNKQCGCRKQKSDCGVDCSCDPTKCRNRQQGKDSLGTVEQTQDSEGSFKLEDPTEVTPGLSFFNPVCATPNSKILKEMCDMEQVLSKKTAPAPSPFDLPESKHGATEYQQNKPPGKKKKRALASNTSFFSGCSPIEEEAH
PTMs - Q2VIQ3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K129 | Ubiquitination | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| T199 | Phosphorylation | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| K401 | Sumoylation | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| K449 | Ubiquitination | Uniprot | |
| K460 | Sumoylation | Uniprot | |
| Y557 | Phosphorylation | Uniprot | |
| K624 | Acetylation | Uniprot | |
| K670 | Ubiquitination | Uniprot | |
| K676 | Ubiquitination | Uniprot | |
| Y684 | Phosphorylation | Uniprot | |
| K688 | Ubiquitination | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K840 | Ubiquitination | Uniprot | |
| K868 | Acetylation | Uniprot | |
| Y869 | Phosphorylation | Uniprot | |
| S876 | Phosphorylation | Uniprot | |
| S877 | Phosphorylation | Uniprot | |
| S961 | Phosphorylation | Uniprot | |
| S1038 | Phosphorylation | Uniprot | |
| K1097 | Ubiquitination | Uniprot | |
| S1106 | Phosphorylation | Uniprot | |
| S1113 | Phosphorylation | Uniprot | |
| T1117 | Phosphorylation | Uniprot | |
| T1135 | Phosphorylation | Uniprot | |
| S1138 | Phosphorylation | Uniprot | |
| S1141 | Phosphorylation | Uniprot | |
| T1148 | Phosphorylation | Uniprot | |
| T1151 | Phosphorylation | Uniprot | |
| T1163 | Phosphorylation | Uniprot | |
| S1166 | Phosphorylation | Uniprot | |
| T1183 | Phosphorylation | Uniprot | |
| T1200 | Phosphorylation | Uniprot | |
| Y1202 | Phosphorylation | Uniprot | |
| S1218 | Phosphorylation | Uniprot | |
| S1221 | Phosphorylation | Uniprot | |
| S1224 | Phosphorylation | Uniprot | |
| S1227 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization (By similarity).
Subcellular Location:
Nucleus matrix. Cytoplasm>Cytoskeleton.
Tissue Specificity:
Specifically expressed in testis.
Family&Domains:
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Chromokinesin subfamily.
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