CHD9 Antibody - #DF9384

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Product: CHD9 Antibody
Catalog: DF9384
Description: Rabbit polyclonal antibody to CHD9
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 326kDa; 326kD(Calculated).
Uniprot: Q3L8U1
RRID: AB_2842580

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
CHD9 Antibody detects endogenous levels of total CHD9.
RRID:
AB_2842580
Cite Format: Affinity Biosciences Cat# DF9384, RRID:AB_2842580.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1810014J18Rik; 9030205D12Rik; A330063D19Rik; AD-013; AD013; ATP dependent helicase CHD9; ATP-dependent helicase; CHD 9; CHD9; Chromatin related mesenchymal modulator; chromatin remodeling factor; Chromatin remodeling factor CHROM1; Chromodomain helicase DNA binding protein 9; Ciprofibrate bound protein p240; CReMM; KIAA0308; KISH2; Kismet homolog 2; mKIAA0308; Peroxisomal proliferator activated receptor A interacting complex 320 kDa protein; PPAR alpha interacting complex protein 320 kDa; PPAR-alpha-interacting complex protein 320 k; PPAR{gamma}-interacting cofactor 320 kDa; PRIC320; Proteinx0008;

Immunogens

Immunogen:
Uniprot:

Q3L8U1(CHD9_HUMAN) >>Visit HPA database.

Gene(ID):
CHD9(80205)
Expression:
Q3L8U1 CHD9_HUMAN:

Widely expressed at low levels. In bone marrow, expression is restricted to osteoprogenitor cells adjacent to mature osteoblasts.

Sequence:
MTDPMMDFFDDANLFGETLEGLSDDAFVQPGPVSLVDELNLGAEFEPLHIDSLNHVQGTPTHQKMTDFEQLNQFDSIKFHHVNQSFGSPAEHVLSPHSQFNCSPIHPQNQPNGLFPDVSDGSPMWGHQTATTISNQNGSPFHQQGHSHSMHQNKSFVAHHDFALFQANEQQTQCTSLRSQQNRNNLNPGQNSLSQSKNFMNVSGPHRVNVNHPPQMTNASNSQQSISMQQFSQTSNPSAHFHKCSSHQEGNFNGPSPNMTSCSVSNSQQFSSHYSFSSNHISPNSLLQSSAVLASNHTNQTLSDFTGSNSFSPHRGIKQESTQHILNPNTSLNSNNFQILHSSHPQGNYSNSKLSPVHMNFPDPVDSGTQMGHFNDHVETNGFSSLEENLLHQVESQTEPFTGLDPEDLLQEGLLPHFDESTFGQDNSSHILDHDLDRQFTSHLVTRPSDMAQTQLQSQARSWHSSFSNHQHLHDRNHLCLQRQPPSSKKSDGSGTYTKLQNTQVRVMSEKKQRKKVESESKQEKANRIISEAIAKAKERGERNIPRVMSPENFPTASVEGKEEKKGRRMKSKPKDKDSKKTKTCSKLKEKTKIGKLIITLGKKQKRKNESSDEISDAEQMPQHTLKDQDSQKRRSNRQIKRKKYAEDIEGKQSEEEVKGSMKIKKNSAPLPGEQPLQLFVENPSEEDAAIVDKILSSRTVKKEISPGVMIDTEEFFVKYKNYSYLHCEWATEEQLLKDKRIQQKIKRFKLRQAQRAHFFADMEEEPFNPDYVEVDRVLEVSFCEDKDTGEPVIYYLVKWCSLPYEDSTWELKEDVDLAKIEEFEQLQASRPDTRRLDRPPSNIWKKIDQSRDYKNGNQLREYQLEGLNWLLFNWYNRRNCILADEMGLGKTIQSITFLYEILLTGIRGPFLIIAPLSTIANWEREFRTWTDINVVVYHGSLISRQMIQQYEMYFRDSQGRIIRGAYRFQAIITTFEMILGGCGELNAIEWRCVIIDEAHRLKNKNCKLLEGLKLMNLEHKVLLTGTPLQNTVEELFSLLHFLEPLRFPSESTFMQEFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKKLAPKEETIIEVELTNIQKKYYRAILEKNFSFLSKGAGQTNVPNLVNTMMELRKCCNHPYLIKGAEEKILGEFRDTYNPAASDFHLQAMIQSAGKLVLIDKLLPKMKAGGHKVLIFSQMVRCLDILEDYLIHKRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQNKAVKVYRLVTRNSYEREMFDRASLKLGLDKAVLQSMSGRESNVGGIQQLSKKEIEDLLRRGAYGAIMEEEDEGSKFCEEDIDQILLRRTKTITIESEGRGSTFAKASFVASGNRTDISLDDPNFWQKWAKKAEIDIEAISGRNSLVIDTPRIRKQTRPFSATKDELAELSEAESEGDEKPKLRRPCDRSNGYGRTECFRVEKNLLVYGWGRWREILSHGRFKRQLNEHDVEIICRALLAYCLVHYRGDEKIKGFIWDLITPTEDGQTRELQNHLGLSAPVPRGRKGKKVKTQTSSFDIQKAEWLRKYNPEQLLQDEGYKKHIKHHCNKVLLRVRMLYYLKQEVIGNECQKVFDGVDASDIDVWVPEPDHSEVPAEWWDFDADKSLLIGVFKHGYEKYNTIRADPALCFLERVGKPDEKAVAAEQRANDYMDGDVEDPEYKPAPAIFKDDIEDDVSSPGDLVIADGDGQLMEGDKVYWPTQSALTTRLRRLITAYQRTNKNRQIQQIQPTFSVPTSVMQPIYEEATLNPKMAAKIERQQRWTRREEADFYRVVSTFGVVFDPDRGQFDWTKFRAMARLHKKTDDSLEKYLYAFMSMCRRVCRLPSKEELVDPNIFIQPITEERASRTLYRIELLRKVREQALRHPQLFERLKLCHPNPDLPVWWECGPHDRDLLIGAAKHGVSRTDYHILRDPELSFMAAQRNYSQSKMAHSRTSTPLLQQYQVALSASPLTSLPRLLDAKGIILEEMKVKSENLKEEPQSSEEESMSSVETRTLIKSEPVSPKNGVLPQATGDQKSGGKCETDRRMVAARTEPLTPNPASKKPRVHKRGSESSSDSDSDSERSSCSSRSSSSSSSSSCSHSRSGSSSSSSSSCSSASSSSSSSTSSSSSSSSSSSEESDSDEEEAQKRESTTHMKAYDEESVASLSTTQDETQDSFQMNNGTPESAYILQGGYMLAASYWPKDRVMINRLDSICQTVLKGKWPSARRSYDANTVASFYTTKLLDSPGAATEYSDPSVPTPPGAGVKEEHDQSTQMSKVKKHVREKEFTVKIKDEGGLKLTFQKQGLAQKRPFDGEDGALGQQQYLTRLRELQSASETSLVNFPKSIPVSGTSIQPTLGANGVILDNQPIVKKRRGRRKNVEGVDIFFFNRNKPPNHVSLGLTSSQISTGINPALSYTQPQGIPDTESPVPVINLKDGTRLAGDDAPKRKDLEKWLKEHPGYVEDLGAFIPRMQLHEGRPKQKRHRCRNPNKLDVNSLTGEERVQLINRRNARKVGGAFAPPLKDLCRFLKENSEYGVAPEWGDVVKQSGFLPESMYERILTGPVVREEVSRRGRRPKSGIAKATAAAAAASATSVSGNPLLANGLLPGVDLTTLQALQQNLQNLQSLQVTAGLMGMPTGLPSGGEAKNMAAMFPMLLSGMAGLPNLLGMGGLLTKPTESGTEDKKGSDSKESEGKTERTESQSSENGGENSVSSSPSTSSTAALNTAAAANPLALNPLLLSNILYPGMLLTPGLNLHIPTLSQSNTFDVQNKNSDLGSSKSVEVKEEDSRIKDQEDKGGTEPSPLNENSTDEGSEKADASSGSDSTSSSSEDSDSSNED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
91
Chicken
91
Horse
67
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q3L8U1 As Substrate

Site PTM Type Enzyme
T2 Phosphorylation
K197 Ubiquitination
S203 Phosphorylation
K318 Sumoylation
K490 Acetylation
K499 Acetylation
K499 Ubiquitination
S521 Phosphorylation
S550 Phosphorylation
S611 Phosphorylation
S612 Phosphorylation
S616 Phosphorylation
T625 Phosphorylation
S654 Phosphorylation
K694 Ubiquitination
K703 Ubiquitination
Y720 Phosphorylation
Y725 Phosphorylation
K738 Methylation
Y772 Phosphorylation
S782 Phosphorylation
K820 Ubiquitination
K846 Ubiquitination
Y951 Phosphorylation
K1008 Ubiquitination
K1014 Ubiquitination
K1069 Ubiquitination
K1075 Ubiquitination
K1116 Ubiquitination
K1151 Ubiquitination
K1156 Ubiquitination
K1189 Ubiquitination
R1251 Methylation
K1323 Ubiquitination
K1328 Ubiquitination
K1349 Ubiquitination
K1350 Ubiquitination
T1387 Phosphorylation
T1389 Phosphorylation
K1403 Ubiquitination
S1405 Phosphorylation
K1425 Ubiquitination
K1429 Sumoylation
K1429 Ubiquitination
S1438 Phosphorylation
T1447 Phosphorylation
S1458 Phosphorylation
S1468 Phosphorylation
S1472 Phosphorylation
K1598 Ubiquitination
K1604 Ubiquitination
Y1635 Phosphorylation
Y1636 Phosphorylation
S1682 Phosphorylation
Y1692 Phosphorylation
K1694 Ubiquitination
Y1695 Phosphorylation
K1716 Ubiquitination
K1738 Sumoylation
S1753 Phosphorylation
S1754 Phosphorylation
T1777 Phosphorylation
T2011 Phosphorylation
T2013 Phosphorylation
Y2019 Phosphorylation
S2026 Phosphorylation
K2038 Sumoylation
K2038 Ubiquitination
S2058 Phosphorylation
S2059 Phosphorylation
S2063 Phosphorylation
K2074 Sumoylation
S2075 Phosphorylation
S2079 Phosphorylation
T2113 Phosphorylation
S2138 Phosphorylation
S2141 Phosphorylation
S2142 Phosphorylation
S2149 Phosphorylation
S2150 Phosphorylation
S2151 Phosphorylation
S2152 Phosphorylation
S2154 Phosphorylation
S2157 Phosphorylation
S2159 Phosphorylation
S2185 Phosphorylation
S2186 Phosphorylation
S2187 Phosphorylation
S2188 Phosphorylation
S2189 Phosphorylation
S2198 Phosphorylation
S2286 Phosphorylation
Y2287 Phosphorylation
S2303 Phosphorylation
K2348 Sumoylation
K2350 Sumoylation
K2361 Ubiquitination
K2367 Ubiquitination
K2588 Ubiquitination
S2737 Phosphorylation
K2843 Sumoylation
T2858 Phosphorylation
S2861 Phosphorylation
S2867 Phosphorylation
T2868 Phosphorylation
S2872 Phosphorylation
S2879 Phosphorylation
S2883 Phosphorylation
S2891 Phosphorylation
S2893 Phosphorylation
S2894 Phosphorylation

Research Backgrounds

Function:

Acts as a transcriptional coactivator for PPARA and possibly other nuclear receptors. Proposed to be a ATP-dependent chromatin remodeling protein. Has DNA-dependent ATPase activity and binds to A/T-rich DNA. Associates with A/T-rich regulatory regions in promoters of genes that participate in the differentiation of progenitors during osteogenesis (By similarity).

PTMs:

Phosphorylated on serine and tyrosine residues.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed at low levels. In bone marrow, expression is restricted to osteoprogenitor cells adjacent to mature osteoblasts.

Subunit Structure:

Interacts with PPARA. Probably interacts with ESR1 and NR1I3.

Family&Domains:

Belongs to the SNF2/RAD54 helicase family.

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