CHD8 Antibody - #DF9383

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Product: CHD8 Antibody
Catalog: DF9383
Description: Rabbit polyclonal antibody to CHD8
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 290kDa; 291kD(Calculated).
Uniprot: Q9HCK8
RRID: AB_2842579

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
CHD8 Antibody detects endogenous levels of total CHD8.
RRID:
AB_2842579
Cite Format: Affinity Biosciences Cat# DF9383, RRID:AB_2842579.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent helicase CHD8; ATP-dependent helicase CHD8; Axis duplication inhibitor; Beta catenin binding protein like; CHD 8; CHD-8; chd8; CHD8_HUMAN; Chromodomain helicase DNA binding protein 8; Chromodomain-helicase-DNA-binding protein 8; DKFZp686N17164; Duplin; Helicase with SNF2 domain 1; HELSNF1; KIAA1564;

Immunogens

Immunogen:
Uniprot:

Q9HCK8(CHD8_HUMAN) >>Visit HPA database.

Gene(ID):
CHD8(57680)
Sequence:
MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASELVPPPEETAPTELSKESTAPAPESITLHDYTTQPASQEQPAQPVLQTSTPTSGLLQVSKSQEILSQGNPFMGVSATAVSSSSAGGQPPQSAPKIVILKAPPSSSVTGAHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLAAKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGATGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQQPPSTQPVTLSSVQQAQIMGPGQSPGQRLSVPVKVVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASSAPHSGGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSAGERLKEEKPKKSKTSGASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRIVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSIDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELKRVNRPQASAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILTEFREACHIIPHDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHAYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKGFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDIVEGVDFDKDCEDPEYKPLQGPPKDQDDEGDPLMMMDEEISVIDGDEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKIEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDTMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGPELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGAPAPSLSRCSTPLLHQQYTSRTASPLPLRPDAPVEKSPEETATQVPSLESLTLKLEHEVVARSRPTPQDYEMRVSPSDTTPLVSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTDQSRSKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSSRRSQEMVTGGILGPGNHLLDSPSLTPGEYGDSPVPTPRSSSAASMAEEEASAVSTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHKLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDVDLETRIPVINKVDGTLLVGEDAPRRAELEMWLQGHPEFAVDPRFLAYMEDRRKQKWQRCKKNNKAELNCLGMEPVQTANSRNGKKGHHTETVFNRVLPGPIAPESSKKRARRMRPDLSKMMALMQGGSTGSLSLHNTFQHSSSGLQSVSSLGHSSATSASLPFMPFVMGGAPSSPHVDSSTMLHHHHHHPHPHHHHHHHPGLRAPGYPSSPVTTASGTTLRLPPLQPEEDDDEDEEDDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
83
Pig
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HCK8 As Substrate

Site PTM Type Enzyme
S125 Phosphorylation
T207 Phosphorylation
T209 Phosphorylation
K270 Ubiquitination
S277 Phosphorylation
T278 Phosphorylation
K285 Ubiquitination
S296 Phosphorylation
K313 Ubiquitination
K328 Ubiquitination
K399 Acetylation
K399 Ubiquitination
S409 Phosphorylation
S423 Phosphorylation
S432 Phosphorylation
S435 Phosphorylation
S440 Phosphorylation
T527 Phosphorylation
S531 Phosphorylation
T535 Phosphorylation
T537 Phosphorylation
T548 Phosphorylation
S549 Phosphorylation
S550 Phosphorylation
S553 Phosphorylation
S562 Phosphorylation
K592 Sumoylation
K609 Sumoylation
K655 Ubiquitination
T663 Phosphorylation
K671 Ubiquitination
K704 Ubiquitination
K738 Ubiquitination
K798 Ubiquitination
K1013 Ubiquitination
K1020 Ubiquitination
K1026 Ubiquitination
K1067 Ubiquitination
K1093 Ubiquitination
Y1099 Phosphorylation
K1140 Ubiquitination
R1202 Methylation
K1250 Acetylation
K1270 Ubiquitination
K1274 Ubiquitination
K1279 Ubiquitination
K1300 Ubiquitination
K1301 Acetylation
K1301 Ubiquitination
K1309 Ubiquitination
K1354 Ubiquitination
R1363 Methylation
K1376 Ubiquitination
K1380 Ubiquitination
K1391 Ubiquitination
T1398 Phosphorylation
S1420 Phosphorylation
S1424 Phosphorylation
S1432 Phosphorylation
S1466 Phosphorylation
T1475 Phosphorylation
K1517 Ubiquitination
S1523 Phosphorylation
S1526 Phosphorylation
K1539 Ubiquitination
S1540 Phosphorylation
K1548 Ubiquitination
K1554 Ubiquitination
K1567 Ubiquitination
S1632 Phosphorylation
K1644 Ubiquitination
K1659 Ubiquitination
K1666 Acetylation
K1666 Ubiquitination
S1679 Phosphorylation
K1689 Ubiquitination
K1697 Sumoylation
K1697 Ubiquitination
K1766 Ubiquitination
S1842 Phosphorylation
S1976 Phosphorylation
S1978 Phosphorylation
S1981 Phosphorylation
T1982 Phosphorylation
Y1989 Phosphorylation
T1990 Phosphorylation
S1991 Phosphorylation
T1993 Phosphorylation
S1995 Phosphorylation
S2008 Phosphorylation
T2012 Phosphorylation
T2014 Phosphorylation
S2018 Phosphorylation
K2025 Ubiquitination
T2037 Phosphorylation
Y2041 Phosphorylation
R2044 Methylation
S2046 Phosphorylation
S2048 Phosphorylation
T2051 Phosphorylation
S2069 Phosphorylation
S2071 Phosphorylation
S2076 Phosphorylation
S2083 Phosphorylation
S2084 Phosphorylation
S2085 Phosphorylation
S2086 Phosphorylation
S2087 Phosphorylation
S2126 Phosphorylation
S2182 Phosphorylation
S2200 Phosphorylation
S2202 Phosphorylation
T2204 Phosphorylation
S2211 Phosphorylation
T2215 Phosphorylation
S2223 Phosphorylation
K2241 Ubiquitination
K2256 Sumoylation
K2262 Ubiquitination
K2267 Ubiquitination
K2269 Ubiquitination
K2286 Acetylation
K2320 Ubiquitination
K2394 Ubiquitination
R2404 Methylation
S2414 Phosphorylation
K2416 Ubiquitination
Y2516 Phosphorylation
S2518 Phosphorylation
S2519 Phosphorylation
T2522 Phosphorylation
S2525 Phosphorylation
S2551 Phosphorylation
S2556 Phosphorylation

Research Backgrounds

Function:

DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription.

PTMs:

Sumoylated.

Subcellular Location:

Nucleus.
Note: Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with p53/TP53, histone H1, CTNNB1, CTCF and PIAS3. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with CHD7. Interacts with FAM124B.

Family&Domains:

Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

References

1). Study of the mechanism by which Xiaoyan decoction combined with E7449 regulates tumorigenesis in lung adenocarcinoma. Journal of cellular and molecular medicine, 2024 (PubMed: 38898581) [IF=5.3]

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