CHD6 Antibody - #DF9382

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Product: CHD6 Antibody
Catalog: DF9382
Description: Rabbit polyclonal antibody to CHD6
Application: WB IHC
Reactivity: Human, Mouse
Mol.Wt.: 305kDa; 305kD(Calculated).
Uniprot: Q8TD26
RRID: AB_2842578

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
CHD6 Antibody detects endogenous levels of total CHD6.
RRID:
AB_2842578
Cite Format: Affinity Biosciences Cat# DF9382, RRID:AB_2842578.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent helicase CHD6; ATP-dependent helicase CHD6; CHD-6; CHD5; CHD6; CHD6_HUMAN; chromodomain helicase DNA binding protein 6; Chromodomain-helicase-DNA-binding protein 6; helicase C-terminal domain- and SNF2 N-terminal domain-containing protein; KIAA1335; Radiation induced gene B protein; Radiation-induced gene B protein; RIGB; RP4-620E11.2;

Immunogens

Immunogen:
Uniprot:

Q8TD26(CHD6_HUMAN) >>Visit HPA database.

Gene(ID):
CHD6(84181)
Expression:
Q8TD26 CHD6_HUMAN:

Widely expressed.

Sequence:
MKMKIQKKEKQLSNLKVLNHSPMSDASVNFDYKSPSPFDCSTDQEEKIEDVASHCLPQKDLYTAEEEAATLFPRKMTSHNGMEDSGGGGTGVKKKRKKKEPGDQEGAAKGSKDREPKPKRKREPKEPKEPRKAKEPKKAKEHKEPKQKDGAKKARKPREASGTKEAKEKRSCTDSAARTKSRKASKEQGPTPVEKKKKGKRKSETTVESLELDQGLTNPSLRSPEESTESTDSQKRRSGRQVKRRKYNEDLDFKVVDDDGETIAVLGAGRTSALSASTLAWQAEEPPEDDANIIEKILASKTVQEVHPGEPPFDLELFYVKYRNFSYLHCKWATMEELEKDPRIAQKIKRFRNKQAQMKHIFTEPDEDLFNPDYVEVDRILEVAHTKDAETGEEVTHYLVKWCSLPYEESTWELEEDVDPAKVKEFESLQVLPEIKHVERPASDSWQKLEKSREYKNSNQLREYQLEGMNWLLFNWYNRKNCILADEMGLGKTIQSITFLSEIFLRGIHGPFLIIAPLSTITNWEREFRTWTEMNAIVYHGSQISRQMIQQYEMVYRDAQGNPLSGVFKFHVVITTFEMILADCPELKKIHWSCVIIDEAHRLKNRNCKLLEGLKLMALEHKVLLTGTPLQNSVEELFSLLNFLEPSQFPSETAFLEEFGDLKTEEQVKKLQSILKPMMLRRLKDDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLTKGANQHNMPNLINTMMELRKCCNHPYLINGAEEKILEDFRKTHSPDAPDFQLQAMIQAAGKLVLIDKLLPKLIAGGHKVLIFSQMVRCLDILEDYLIQRRYTYERIDGRVRGNLRQAAIDRFCKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQDINRKGGTNGVQQLSKMEVEDLLRKGAYGALMDEEDEGSKFCEEDIDQILQRRTHTITIQSEGKGSTFAKASFVASGNRTDISLDDPNFWQKWAKIAELDTEAKNEKESLVIDRPRVRKQTKHYNSFEEDELMEFSELDSDSDERPTRSRRLNDKARRYLRAECFRVEKNLLIFGWGRWKDILTHGRFKWHLNEKDMEMICRALLVYCVKHYKGDEKIKSFIWELITPTKDGQAQTLQNHSGLSAPVPRGRKGKKTKNQLLIPELKDADWLATCNPEVVLHDDGYKKHLKQHCNKVLLRVRMLYYLKAEILGEAAEKAFEGSPARELDVPLPDIDYMEIPVDWWDAEADKSLLIGVFKHGYERYNAMRADPALCFLEKVGMPDEKSLSAEQGVTDGTSDIPERGNTDKEDNAEDKVDGLQKQTESSSDGGDGVFSEKKDDSRAAQDGSDPDKSPWPVSSALTARLRRLVTVYQRCNRKELCRPEILGPGNQGYWVQEEMFRRTSEMDLINKEAQKRWTRREQADFYRTVSSFGVVYDQEKKTFDWTQFRIISRLDKKSDESLEQYFYSFVAMCRNVCRLPTWKDGGPPDTTIYVEPITEERAARTLYRIELLRKVREQVLKCPQLHERLQLCRPSLYLPVWWECGKHDRDLLIGTAKHGLNRTDCYIMNDPQLSFLDAYRNYAQHKRSGTQAPGNLCCLYQTNSKLYESLTYSQMSRTSESLENEPENLVRVESRDDHLSLPDVTCENFISKVQDVISINHDESLLPESLESMMYGKKVLSQEPSSFQESPSTNTESRKDVITISISKDGNCQSGGPEAEIASGPTFMGSLEAGGVAQANIKNGKHLLMSISKEGELCCSEAGQRPENIGQLEAKCLASPSLNPGNESGFVDMCSLSVCDSKRNLSSDQQLIDLLENKSLESKLILSQNHSDEEEEEEENEEENLAMAVGMGERPEVLHLTEPTTNISREKNQGFQDETKKGSLEVANQTPGLQRAFPAPAACQCHCKHMERWMHGLENDEFEIEKPKAYIPDLFKSKTNTIAMEGEPTAIPSQPFKVKHELLKEPWKESAEGQNVFPTYPLEGSELKSEDMDFENKDDYDRDGNCHSQDYPGKYSEEESKSSTSGITGDIGDELQEARAPTIAQLLQEKTLYSFSEWPKDRVIINRLDNICHVVLKGKWPSSQQYEPSGTLPTPVLTSSAGSRTSLSEPEAAEHSFSNGAALAAQIHKESFLAPVFTKDEQKHRRPYEFEVERDAKARGLEQFSATHGHTPIILNGWHGESAMDLSCSSEGSPGATSPFPVSASTPKIGAISSLQGALGMDLSGILQAGLIHPVTGQIVNGSLRRDDAATRRRRGRRKHVEGGMDLIFLKEQTLQAGILEVHEDPGQATLSTTHPEGPGPATSAPEPATAASSQAEKSIPSKSLLDWLRQQADYSLEVPGFGANFSDKPKQRRPRCKEPGKLDVSSLSGEERVPAIPKEPGLRGFLPENKFNHTLAEPILRDTGPRRRGRRPRSELLKAPSIVADSPSGMGPLFMNGLIAGMDLVGLQNMRNMPGIPLTGLVGFPAGFATMPTGEEVKSTLSMLPMMLPGMAAVPQMFGVGGLLSPPMATTCTSTAPASLSSTTKSGTAVTEKTAEDKPSSHDVKTDTLAEDKPGPGPFSDQSEPAITTSSPVAFNPFLIPGVSPGLIYPSMFLSPGMGMALPAMQQARHSEIVGLESQKRKKKKTKGDNPNSHPEPAPSCEREPSGDENCAEPSAPLPAEREHGAQAGEGALKDSNNDTN

PTMs - Q8TD26 As Substrate

Site PTM Type Enzyme
K10 Acetylation
S21 Phosphorylation
S24 Phosphorylation
S27 Phosphorylation
S36 Phosphorylation
S41 Phosphorylation
T42 Phosphorylation
S53 Phosphorylation
T77 Phosphorylation
S78 Phosphorylation
S85 Phosphorylation
S111 Phosphorylation
K134 Ubiquitination
K138 Ubiquitination
K183 Acetylation
S185 Phosphorylation
K186 Acetylation
K198 Acetylation
T262 Phosphorylation
K296 Ubiquitination
K301 Ubiquitination
Y327 Phosphorylation
T334 Phosphorylation
K340 Acetylation
K424 Ubiquitination
K436 Ubiquitination
K448 Ubiquitination
T493 Phosphorylation
S565 Phosphorylation
K609 Ubiquitination
K615 Ubiquitination
K670 Ubiquitination
K717 Ubiquitination
K743 Ubiquitination
Y749 Phosphorylation
K790 Ubiquitination
K900 Acetylation
K920 Ubiquitination
K924 Ubiquitination
K929 Ubiquitination
T941 Phosphorylation
K958 Ubiquitination
K1003 Ubiquitination
K1025 Ubiquitination
K1028 Ubiquitination
K1040 Ubiquitination
S1069 Phosphorylation
S1073 Phosphorylation
S1174 Phosphorylation
S1177 Phosphorylation
K1199 Ubiquitination
Y1237 Phosphorylation
Y1238 Phosphorylation
K1250 Ubiquitination
S1284 Phosphorylation
K1311 Ubiquitination
T1327 Phosphorylation
T1339 Phosphorylation
K1444 Ubiquitination
Y1459 Phosphorylation
Y1469 Phosphorylation
S1491 Phosphorylation
Y1500 Phosphorylation
Y1540 Phosphorylation
K1590 Ubiquitination
Y1640 Phosphorylation
Y1645 Phosphorylation
S1654 Phosphorylation
S1667 Phosphorylation
S1673 Phosphorylation
S1684 Phosphorylation
K1710 Ubiquitination
K1711 Ubiquitination
S1714 Phosphorylation
S1719 Phosphorylation
S1723 Phosphorylation
S1725 Phosphorylation
T1728 Phosphorylation
S1785 Phosphorylation
S1839 Phosphorylation
S1840 Phosphorylation
K1851 Ubiquitination
S1852 Phosphorylation
S1855 Phosphorylation
T1897 Phosphorylation
T1898 Phosphorylation
T1923 Phosphorylation
K1959 Ubiquitination
T1972 Phosphorylation
K1992 Sumoylation
K1997 Ubiquitination
S2041 Phosphorylation
K2083 Ubiquitination
K2172 Ubiquitination
Y2181 Phosphorylation
K2356 Ubiquitination
S2402 Phosphorylation
K2412 Ubiquitination
R2417 Methylation
K2424 Ubiquitination
S2652 Phosphorylation
K2654 Ubiquitination
S2674 Phosphorylation
S2680 Phosphorylation
S2710 Phosphorylation
T2714 Phosphorylation

Research Backgrounds

Function:

DNA-dependent ATPase that plays a role in chromatin remodeling. Regulates transcription by disrupting nucleosomes in a largely non-sliding manner which strongly increases the accessibility of chromatin. Activates transcription of specific genes in response to oxidative stress through interaction with NFE2L2.

(Microbial infection) Acts as a transcriptional repressor of different viruses including influenza virus or papillomavirus. During influenza virus infection, the viral polymerase complex localizes CHD6 to inactive chromatin where it gets degraded in a proteasome independent-manner.

Subcellular Location:

Nucleus>Nucleoplasm.
Note: Enriched at sites of mRNA synthesis (PubMed:17027977). During influenza A virus infection, localizes to inactive chromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts with NFE2L2; involved in activation of the transcription.

(Microbial infection) Interacts with the influenza A polymerase complex composed fo PB1, PB2 and PA.

(Microbial infection) Interacts (via N-terminus) with human papillomavirus protein E8^E2C (via C-terminus); this interaction induces transcriptional repression of the viral genome.

Family&Domains:

Belongs to the SNF2/RAD54 helicase family.

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