CHD2 Antibody - #DF9381

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Product: CHD2 Antibody
Catalog: DF9381
Description: Rabbit polyclonal antibody to CHD2
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 211kDa; 211kD(Calculated).
Uniprot: O14647
RRID: AB_2842577

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%)
Clonality:
Polyclonal
Specificity:
CHD2 Antibody detects endogenous levels of total CHD2.
RRID:
AB_2842577
Cite Format: Affinity Biosciences Cat# DF9381, RRID:AB_2842577.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2810013C04Rik; 2810040A01Rik; 5630401D06Rik; AI851092; ATP dependent helicase CHD2; ATP-dependent helicase CHD2; BC029703; CHD 2; CHD-2; CHD2; CHD2_HUMAN; chromodomain helicase dna binding protein; Chromodomain-helicase-DNA-binding protein 2; DKFZp547I1315; DKFZp686E01200; DKFZP781D1727; EC 3.6.1.-; EEOC; FLJ38614;

Immunogens

Immunogen:
Uniprot:

O14647(CHD2_HUMAN) >>Visit HPA database.

Gene(ID):
CHD2(1106)
Sequence:
MMRNKDKSQEEDSSLHSNASSHSASEEASGSDSGSQSESEQGSDPGSGHGSESNSSSESSESQSESESESAGSKSQPVLPEAKEKPASKKERIADVKKMWEEYPDVYGVRRSNRSRQEPSRFNIKEEASSGSESGSPKRRGQRQLKKQEKWKQEPSEDEQEQGTSAESEPEQKKVKARRPVPRRTVPKPRVKKQPKTQRGKRKKQDSSDEDDDDDEAPKRQTRRRAAKNVSYKEDDDFETDSDDLIEMTGEGVDEQQDNSETIEKVLDSRLGKKGATGASTTVYAIEANGDPSGDFDTEKDEGEIQYLIKWKGWSYIHSTWESEESLQQQKVKGLKKLENFKKKEDEIKQWLGKVSPEDVEYFNCQQELASELNKQYQIVERVIAVKTSKSTLGQTDFPAHSRKPAPSNEPEYLCKWMGLPYSECSWEDEALIGKKFQNCIDSFHSRNNSKTIPTRECKALKQRPRFVALKKQPAYLGGENLELRDYQLEGLNWLAHSWCKNNSVILADEMGLGKTIQTISFLSYLFHQHQLYGPFLIVVPLSTLTSWQREFEIWAPEINVVVYIGDLMSRNTIREYEWIHSQTKRLKFNALITTYEILLKDKTVLGSINWAFLGVDEAHRLKNDDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFEFWEDFEEDHGKGRENGYQSLHKVLEPFLLRRVKKDVEKSLPAKVEQILRVEMSALQKQYYKWILTRNYKALAKGTRGSTSGFLNIVMELKKCCNHCYLIKPPEENERENGQEILLSLIRSSGKLILLDKLLTRLRERGNRVLIFSQMVRMLDILAEYLTIKHYPFQRLDGSIKGEIRKQALDHFNADGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGTVEEEIIERAKKKMVLDHLVIQRMDTTGRTILENNSGRSNSNPFNKEELTAILKFGAEDLFKELEGEESEPQEMDIDEILRLAETRENEVSTSATDELLSQFKVANFATMEDEEELEERPHKDWDEIIPEEQRKKVEEEERQKELEEIYMLPRIRSSTKKAQTNDSDSDTESKRQAQRSSASESETEDSDDDKKPKRRGRPRSVRKDLVEGFTDAEIRRFIKAYKKFGLPLERLECIARDAELVDKSVADLKRLGELIHNSCVSAMQEYEEQLKENASEGKGPGKRRGPTIKISGVQVNVKSIIQHEEEFEMLHKSIPVDPEEKKKYCLTCRVKAAHFDVEWGVEDDSRLLLGIYEHGYGNWELIKTDPELKLTDKILPVETDKKPQGKQLQTRADYLLKLLRKGLEKKGAVTGGEEAKLKKRKPRVKKENKVPRLKEEHGIELSSPRHSDNPSEEGEVKDDGLEKSPMKKKQKKKENKENKEKQMSSRKDKEGDKERKKSKDKKEKPKSGDAKSSSKSKRSQGPVHITAGSEPVPIGEDEDDDLDQETFSICKERMRPVKKALKQLDKPDKGLNVQEQLEHTRNCLLKIGDRIAECLKAYSDQEHIKLWRRNLWIFVSKFTEFDARKLHKLYKMAHKKRSQEEEEQKKKDDVTGGKKPFRPEASGSSRDSLISQSHTSHNLHPQKPHLPASHGPQMHGHPRDNYNHPNKRHFSNADRGDWQRERKFNYGGGNNNPPWGSDRHHQYEQHWYKDHHYGDRRHMDAHRSGSYRPNNMSRKRPYDQYSSDRDHRGHRDYYDRHHHDSKRRRSDEFRPQNYHQQDFRRMSDHRPAMGYHGQGPSDHYRSFHTDKLGEYKQPLPPLHPAVSDPRSPPSQKSPHDSKSPLDHRSPLERSLEQKNNPDYNWNVRKT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14647 As Substrate

Site PTM Type Enzyme
S75 Phosphorylation
K83 Acetylation
K85 Acetylation
K90 Acetylation
S129 Phosphorylation
S130 Phosphorylation
S132 Phosphorylation
S136 Phosphorylation
S156 Phosphorylation
T164 Phosphorylation
S165 Phosphorylation
S168 Phosphorylation
S207 Phosphorylation
S208 Phosphorylation
T240 Phosphorylation
S242 Phosphorylation
T262 Phosphorylation
S269 Phosphorylation
T282 Phosphorylation
K354 Ubiquitination
T396 Phosphorylation
S402 Phosphorylation
K436 Ubiquitination
K472 Ubiquitination
Y596 Phosphorylation
K623 Ubiquitination
K631 Ubiquitination
K637 Ubiquitination
S708 Phosphorylation
S722 Phosphorylation
Y728 Phosphorylation
Y729 Phosphorylation
S747 Phosphorylation
Y766 Phosphorylation
T828 Phosphorylation
S840 Phosphorylation
S1012 Phosphorylation
S1019 Phosphorylation
T1028 Phosphorylation
K1062 Ubiquitination
Y1068 Phosphorylation
T1082 Phosphorylation
S1085 Phosphorylation
S1087 Phosphorylation
T1089 Phosphorylation
S1091 Phosphorylation
T1105 Phosphorylation
S1108 Phosphorylation
T1132 Phosphorylation
K1141 Ubiquitination
K1165 Ubiquitination
T1209 Phosphorylation
S1213 Phosphorylation
K1295 Ubiquitination
K1338 Acetylation
S1364 Phosphorylation
S1365 Phosphorylation
S1369 Phosphorylation
S1373 Phosphorylation
S1386 Phosphorylation
S1429 Phosphorylation
S1434 Phosphorylation
S1441 Phosphorylation
T1448 Phosphorylation
R1546 Methylation
S1584 Phosphorylation
S1590 Phosphorylation
S1633 Phosphorylation
R1637 Methylation
R1685 Methylation
S1695 Phosphorylation
Y1715 Phosphorylation
Y1716 Phosphorylation
S1728 Phosphorylation
Y1736 Phosphorylation
S1745 Phosphorylation
Y1753 Phosphorylation
Y1762 Phosphorylation
S1785 Phosphorylation
S1789 Phosphorylation
S1792 Phosphorylation
S1795 Phosphorylation
S1799 Phosphorylation
S1801 Phosphorylation
S1807 Phosphorylation
Y1821 Phosphorylation
T1828 Phosphorylation

Research Backgrounds

Function:

DNA-binding helicase that specifically binds to the promoter of target genes, leading to chromatin remodeling, possibly by promoting deposition of histone H3.3. Involved in myogenesis via interaction with MYOD1: binds to myogenic gene regulatory sequences and mediates incorporation of histone H3.3 prior to the onset of myogenic gene expression, promoting their expression (By similarity).

Subcellular Location:

Nucleus.
Note: Binds to myogenic gene promoters.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with MYOD1. Interacts with histone H3.3 (By similarity).

Family&Domains:

Belongs to the SNF2/RAD54 helicase family.

Restrictive clause

 

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