CEP250 Antibody - #DF9367

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Product: CEP250 Antibody
Catalog: DF9367
Description: Rabbit polyclonal antibody to CEP250
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 281kDa; 281kD(Calculated).
Uniprot: Q9BV73
RRID: AB_2842563

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(90%), Horse(80%), Sheep(90%), Dog(90%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
CEP250 Antibody detects endogenous levels of total CEP250.
RRID:
AB_2842563
Cite Format: Affinity Biosciences Cat# DF9367, RRID:AB_2842563.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:

Q9BV73(CP250_HUMAN) >>Visit HPA database.

Gene(ID):
CEP250(11190)
Expression:
Q9BV73 CP250_HUMAN:

Ubiquitously and weakly expressed.

Sequence:
METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
90
Sheep
90
Dog
90
Chicken
88
Horse
80
Rabbit
70
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BV73 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation
K12 Acetylation
S45 Phosphorylation
T54 Phosphorylation
K62 Ubiquitination
K75 Ubiquitination
K126 Ubiquitination
K132 Ubiquitination
K155 Ubiquitination
K167 Ubiquitination
T186 Phosphorylation
K206 Ubiquitination
T253 Phosphorylation
S264 Phosphorylation
K271 Ubiquitination
T293 Phosphorylation
S295 Phosphorylation
K297 Acetylation
K297 Ubiquitination
K338 Ubiquitination
K463 Ubiquitination
K588 Ubiquitination
K600 Ubiquitination
K652 Ubiquitination
S699 Phosphorylation
T842 Phosphorylation
K892 Ubiquitination
S929 Phosphorylation
T933 Phosphorylation
K956 Ubiquitination
T971 Phosphorylation
K1010 Ubiquitination
K1016 Ubiquitination
K1036 Ubiquitination
T1040 Phosphorylation
K1048 Acetylation
K1048 Ubiquitination
K1148 Ubiquitination
K1254 Ubiquitination
K1268 Ubiquitination
K1280 Ubiquitination
S1295 Phosphorylation
S1310 Phosphorylation
S1313 Phosphorylation
S1324 Phosphorylation
S1386 Phosphorylation
K1412 Ubiquitination
K1417 Ubiquitination
K1449 Ubiquitination
K1466 Ubiquitination
K1484 Ubiquitination
K1502 Ubiquitination
K1533 Ubiquitination
K1574 Ubiquitination
K1577 Ubiquitination
S1601 Phosphorylation
S1609 Phosphorylation
S1618 Phosphorylation
K1664 Ubiquitination
K1679 Ubiquitination
K1689 Ubiquitination
T1701 Phosphorylation
S1723 Phosphorylation
K1797 Ubiquitination
T1853 Phosphorylation
K1855 Ubiquitination
K1865 Ubiquitination
K1901 Ubiquitination
S1927 Phosphorylation
K1936 Ubiquitination
K1972 Ubiquitination
K1980 Ubiquitination
K2063 Ubiquitination
K2077 Ubiquitination
S2095 Phosphorylation
S2128 Phosphorylation
K2130 Ubiquitination
S2138 Phosphorylation
K2140 Ubiquitination
S2143 Phosphorylation
K2172 Ubiquitination
S2179 Phosphorylation
T2218 Phosphorylation
S2229 Phosphorylation
T2233 Phosphorylation
S2234 Phosphorylation
S2252 Phosphorylation
S2259 Phosphorylation
K2265 Ubiquitination
S2267 Phosphorylation
S2322 Phosphorylation
K2327 Ubiquitination
S2332 Phosphorylation
K2347 Ubiquitination
T2364 Phosphorylation
Y2373 Phosphorylation
S2390 Phosphorylation
S2392 Phosphorylation P51955 (NEK2) , Q9H0K1 (SIK2)
S2394 Phosphorylation Q9H0K1 (SIK2) , P51955 (NEK2)
S2417 Phosphorylation P51955 (NEK2)
T2419 Phosphorylation
S2421 Phosphorylation P51955 (NEK2)
S2424 Phosphorylation
S2433 Phosphorylation
T2437 Phosphorylation

Research Backgrounds

Function:

May be involved in ciliogenesis. Probably plays an important role in centrosome cohesion during interphase.

PTMs:

Differentially phosphorylated during cell cycle. Phosphorylation may regulate association/dissociation from centrosome. During M phase of mitosis, C-terminal part is phosphorylated by NEK2, suggesting that it may trigger the dissociation from the mitotic centrosome. Dephosphorylated in vitro by the PP1 phosphatase.

Subcellular Location:

Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Cilium basal body. Cell projection>Cilium>Photoreceptor outer segment. Photoreceptor inner segment.
Note: Component of the core centrosome. In interphase cells, it specifically associates with the proximal ends of both mother and daughter centrioles. Associates with the centrosome in interphase cells. In mitotic cells, it dissociates from the mitotic spindle poles. At the end of cell division, it reaccumulates at centrosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously and weakly expressed.

Subunit Structure:

Monomer and homodimer (Probable). Forms a complex in vitro with both NEK2 kinase and the PPP1CC catalytic subunit of protein phosphatase 1 (PP1). Interacts with CEP135. Interacts with CROCC/rootletin (By similarity). Interacts with CNTLN. Interacts with NIN (via C-terminus) (By similarity).

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