CSPP1 Antibody - #DF9366
(3)
| Product: | CSPP1 Antibody |
| Catalog: | DF9366 |
| Description: | Rabbit polyclonal antibody to CSPP1 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit |
| Mol.Wt.: | 146 kDa; 146kD(Calculated). |
| Uniprot: | Q1MSJ5 |
| RRID: | AB_2842562 |
Product Info
Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
CSPP1 Antibody detects endogenous levels of total CSPP1.
RRID:
AB_2842562
Cite Format: Affinity Biosciences Cat# DF9366, RRID:AB_2842562.
Cite Format: Affinity Biosciences Cat# DF9366, RRID:AB_2842562.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Centrosome and spindle pole associated protein 1; CSPP 1; CSPP; FLJ22490; FLJ38886;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Q1MSJ5 CSPP1_HUMAN:
Expressed in adult and fetal brain with enrichment in the cerebellum. Detected in testis.
Sequence:
- Q1MSJ5 CSPP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLFPLQVAAVTSSVRDDPLEHCVSPRTRARSPEICKMADNLDEFIEEQKARLAEDKAELESDPPYMEMKGKLSAKLSENSKILISMAKENIPPNSQQTRGSLGIDYGLSLPLGEDYERKKHKLKEELRQDYRRYLTQGITQGKRKKNFLSTSETDPSTLGVSLPIGERLSAKERLKLERNKEYNQFLRGKEESSEKFRQVEKSTEPKSQRNKKPIGQVKPDLTSQIQTSCENSEGPRKDVLTPSEAYEELLNQRRLEEDRYRQLDDEIELRNRRIIKKANEEVGISNLKHQRFASKAGIPDRRFHRFNEDRVFDRRYHRPDQDPEVSEEMDERFRYESDFDRRLSRVYTNDRMHRNKRGNMPPMEHDGDVIEQSNIRISSAENKSAPDNETSKSANQDTCSPFAGMLFGGEDRELIQRRKEKYRLELLEQMAEQQRNKRREKDLELRVAASGAQDPEKSPDRLKQFSVAPRHFEEMIPPERPRIAFQTPLPPLSAPSVPPIPSVHPVPSQNEDLRSGLSSALGEMVSPRIAPLPPPPLLPPLATNYRTPYDDAYYFYGSRNTFDPSLAYYGSGMMGVQPAAYVSAPVTHQLAQPVVNTVGQNELKITSDQVINSGLIFEDKPKPSKQSLQSYQEALQQQIREREERRKKEREEKEEYEAKLEAEMRTYNPWGKGGGGAPLRDAKGNLITDLNRMHRQNIDAYHNPDARTYEDKRAVVSLDPNLATSNAENLEDAANKSSGHMQTQSSPFARGNVFGEPPTELQIKQQELYKNFLRFQIEEKKQREEAERERLRIAEEKEERRLAEQRARIQQEYEEEQEKKREKEEEQRLKNEEHIRLAEERQKEAERKKKEEEEKYNLQLQHYCERDNLIGEETKHMRQPSPIVPALQNKIASKLQRPPSVDSIIRSFIHESSMSRAQSPPVPARKNQLRAEEEKKNVIMELSEMRKQLRSEERRLQERLLHMDSDDEIPIRKKERNPMDIFDMARHRLQAPVRRQSPKGLDAATFQNVHDFNELKDRDSETRVDLKFMYLDPPRDHHTLEIQQQALLREQQKRLNRIKMQEGAKVDLDAIPSAKVREQRMPRDDTSDFLKNSLLESDSAFIGAYGETYPAIEDDVLPPPSQLPSARERRRNKWKGLDIDSSRPNVAPDGLSLKSISSVNVDELRVRNEERMRRLNEFHNKPINTDDESSLVDPDDIMKHIGDDGSNSVATEPWLRPGTSETLKRFMAEQLNQEQQQIPGKPGTFTWQGLSTAHG
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q1MSJ5 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S31 | Phosphorylation | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| Y65 | Phosphorylation | Uniprot | |
| K69 | Methylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| K278 | Methylation | Uniprot | |
| K289 | Methylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| Y336 | Phosphorylation | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| S527 | Phosphorylation | Uniprot | |
| Y554 | Phosphorylation | Uniprot | |
| Y555 | Phosphorylation | Uniprot | |
| Y557 | Phosphorylation | Uniprot | |
| K621 | Ubiquitination | Uniprot | |
| R641 | Methylation | Uniprot | |
| R643 | Methylation | Uniprot | |
| K648 | Acetylation | Uniprot | |
| K649 | Acetylation | Uniprot | |
| Y668 | Phosphorylation | Uniprot | |
| K673 | Ubiquitination | Uniprot | |
| K684 | Ubiquitination | Uniprot | |
| Y702 | Phosphorylation | Uniprot | |
| S747 | Phosphorylation | Uniprot | |
| K765 | Ubiquitination | Uniprot | |
| S882 | Phosphorylation | Uniprot | |
| S901 | Phosphorylation | Uniprot | |
| S904 | Phosphorylation | Uniprot | |
| S920 | Phosphorylation | Uniprot | |
| S966 | Phosphorylation | Uniprot | |
| K1017 | Ubiquitination | Uniprot | |
| K1060 | Ubiquitination | Uniprot | |
| K1066 | Ubiquitination | Uniprot | |
| K1076 | Ubiquitination | Uniprot | |
| K1136 | Ubiquitination | Uniprot | |
| K1155 | Ubiquitination | Uniprot | |
| S1156 | Phosphorylation | Uniprot | |
| T1186 | Phosphorylation | Uniprot | |
| S1191 | Phosphorylation | Uniprot | |
| K1225 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
May play a role in cell-cycle-dependent microtubule organization.
PTMs:
Phosphorylated. Phosphorylation increases in colcemide-treated cells.
Subcellular Location:
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Associated with mitotic spindles.
Tissue Specificity:
Expressed in adult and fetal brain with enrichment in the cerebellum. Detected in testis.
Subunit Structure:
Interacts with PLEKHG6.
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