CEP72 Antibody - #DF9365

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Product: CEP72 Antibody
Catalog: DF9365
Description: Rabbit polyclonal antibody to CEP72
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Monkey
Mol.Wt.: 72 kDa; 72kD(Calculated).
Uniprot: Q9P209
RRID: AB_2842561

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Clonality:
Polyclonal
Specificity:
CEP72 Antibody detects endogenous levels of total CEP72.
RRID:
AB_2842561
Cite Format: Affinity Biosciences Cat# DF9365, RRID:AB_2842561.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Centrosomal protein 72kDa; Centrosomal protein of 72 kDa; Cep72; CEP72_HUMAN; FLJ10565; MGC5307; RGD1566336;

Immunogens

Immunogen:
Uniprot:

Q9P209(CEP72_HUMAN) >>Visit HPA database.

Gene(ID):
CEP72(55722)
Sequence:
MARAGPRLVLSEEAVRAKSGLGPHRDLAELQSLSIPGTYQEKITHLGHSLMSLTGLKSLDLSRNSLVSLEGIQYLTALESLNLYYNCISSLAEVFRLHALTELVDVDFRLNPVVKVEPDYRLFVVHLLPKLQQLDDRPVRASERKASRLHFASEDSLDSKESVPASLKEGRPHHPRAKCTEALAKQSLVMDADDEAVLNLIAECEWDLGRPPGSTSFSQKGREADSRGSQESRHLLSPQLVQYQCGDSGKQGRETRRSSCRGCCLEKMPWSQLCGELPPLYGAEPEASRAPRPHTYFTPHPDSMDTEDSASSQKLDLSGEMVPGPLPAPGKCRKRRMPVGRFQTFSDQEGLGCPERTHGSSVPKESLSRQDSSESRNGRTLSQPEASETEEQRSRGVTDTREPSPGSHSALPGKKTALQAALLETLLDLVDRSWGGCRSLHSNEAFLAQARHILSSVEEFTAAQDSSAMVGEDVGSLALESKSLQSRLAEQQQQHAREMSEVTAELHHTHKELDDLRQHLDKSLEENSRLKSLLLSMKKEVKSADTAATLNLQIAGLQTSVKRLCGEIVELKQHLEHYDKIQELTQMLQESHSSLVSTNEHLLQELSQVRAQHRAEVEQMHWSYQELKKTMALFPHSSASHGGCQAC

PTMs - Q9P209 As Substrate

Site PTM Type Enzyme
K18 Ubiquitination
T44 Phosphorylation
K57 Ubiquitination
S62 Phosphorylation
K115 Ubiquitination
K130 Ubiquitination
S142 Phosphorylation
S153 Phosphorylation
S156 Phosphorylation
K160 Sumoylation
K160 Ubiquitination
K168 Ubiquitination
K178 Ubiquitination
S237 Phosphorylation
S248 Phosphorylation
K250 Ubiquitination
K267 Ubiquitination
K314 Ubiquitination
S318 Phosphorylation
T344 Phosphorylation
S346 Phosphorylation
K364 Sumoylation
K364 Ubiquitination
S368 Phosphorylation
S372 Phosphorylation
S375 Phosphorylation
T380 Phosphorylation
S382 Phosphorylation
S387 Phosphorylation
T400 Phosphorylation
S404 Phosphorylation
S407 Phosphorylation
K414 Ubiquitination
S439 Phosphorylation
K482 Ubiquitination
K522 Ubiquitination
S523 Phosphorylation
K531 Ubiquitination
S532 Phosphorylation
S536 Phosphorylation
K538 Ubiquitination
K542 Ubiquitination
K562 Ubiquitination
K572 Ubiquitination
K628 Ubiquitination
K629 Ubiquitination

Research Backgrounds

Function:

Involved in the recruitment of key centrosomal proteins to the centrosome. Provides centrosomal microtubule-nucleation activity on the gamma-tubulin ring complexes (gamma-TuRCs) and has critical roles in forming a focused bipolar spindle, which is needed for proper tension generation between sister chromatids. Required for localization of KIZ, AKAP9 and gamma-tubulin ring complexes (gamma-TuRCs). Involved in centriole duplication. Required for CDK5RAP22, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite.
Note: Localizes to the centrosome and centrosome-surrounding particles throughout the cell cycle. These particles disappear after microtubules are depolymerized using nocodazole, suggesting that CEP72-associating particles localize in a microtubule- dependent manner.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with KIZ, PCM1 and CDK5RAP2.

Family&Domains:

Belongs to the CEP72 family.

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