CEP63 Antibody - #DF9363
Product: | CEP63 Antibody |
Catalog: | DF9363 |
Description: | Rabbit polyclonal antibody to CEP63 |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse |
Prediction: | Dog |
Mol.Wt.: | 81 kDa; 81kD(Calculated). |
Uniprot: | Q96MT8 |
RRID: | AB_2842559 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9363, RRID:AB_2842559.
Fold/Unfold
Centrosomal protein 63kDa; Centrosomal protein of 63 kDa; centrosome protein CEP63;
Immunogens
- Q96MT8 CEP63_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEALLEGIQNRGHGGGFLTSCEAELQELMKQIDIMVAHKKSEWEGRTHALETCLKIREQELKSLRSQLDVTHKEVGMLHQQVEEHEKIKQEMTMEYKQELKKLHEELCILKRSYEKLQKKQMREFRGNTKNHREDRSEIERLTAKIEEFRQKSLDWEKQRLIYQQQVSSLEAQRKALAEQSEIIQAQLVNRKQKLESVELSSQSEIQHLSSKLERANDTICANELEIERLTMRVNDLVGTSMTVLQEQQQKEEKLRESEKLLEALQEEKRELKAALQSQENLIHEARIQKEKLQEKVKATNTQHAVEAIRPREESLAEKKYTSQGQGDLDSVLSQLNFTHTSEDLLQAEVTCLEGSLESVSATCKQLSQELMEKYEELKRMEAHNNEYKAEIKKLKEQILQGEQSYSSALEGMKMEISHLTQELHQRDITIASTKGSSSDMEKRLRAEMQKAEDKAVEHKEILDQLESLKLENRHLSEMVMKLELGLHEAKEISLADLQENYIEALNKLVSENQQLQKDLMNTKSQLEISTQMCKKQNDRIFKPTHSRTTEFKNTEFKPTHGQHRHDGIKTEHYKTDLHSPRGQASDSINPMSRVLSPLSPQISPCSSTRSLTSYSLCKTHSLPSALDTNEANFSDTMSESMNDQEEFISSCSLPVSPLGSIATRFLEEEELRSHHILERLDAHIEELKRESEKTVRQFTALK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96MT8 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
K55 | Ubiquitination | Uniprot | |
K62 | Ubiquitination | Uniprot | |
K73 | Ubiquitination | Uniprot | |
K145 | Ubiquitination | Uniprot | |
K152 | Ubiquitination | Uniprot | |
K158 | Ubiquitination | Uniprot | |
K175 | Ubiquitination | Uniprot | |
K212 | Ubiquitination | Uniprot | |
K251 | Ubiquitination | Uniprot | |
K260 | Ubiquitination | Uniprot | |
K269 | Ubiquitination | Uniprot | |
K273 | Ubiquitination | Uniprot | |
S278 | Phosphorylation | Uniprot | |
K290 | Ubiquitination | Uniprot | |
K389 | Ubiquitination | Uniprot | |
K396 | Ubiquitination | Uniprot | |
S405 | Phosphorylation | Uniprot | |
K414 | Ubiquitination | Uniprot | |
K435 | Ubiquitination | Uniprot | |
K460 | Ubiquitination | Uniprot | |
K470 | Ubiquitination | Uniprot | |
K508 | Ubiquitination | Uniprot | |
T576 | Phosphorylation | Uniprot | |
S586 | Phosphorylation | Uniprot | |
S650 | Phosphorylation | Uniprot | |
S653 | Phosphorylation | Uniprot | |
S657 | Phosphorylation | Uniprot | |
K689 | Ubiquitination | Uniprot |
Research Backgrounds
Required for normal spindle assembly. Plays a key role in mother-centriole-dependent centriole duplication; the function seems also to involve CEP152, CDK5RAP2 and WDR62 through a stepwise assembled complex at the centrosome that recruits CDK2 required for centriole duplication. Reported to be required for centrosomal recruitment of CEP152; however, this function has been questioned. Also recruits CDK1 to centrosomes. Plays a role in DNA damage response. Following DNA damage, such as double-strand breaks (DSBs), is removed from centrosomes; this leads to the inactivation of spindle assembly and delay in mitotic progression.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite.
Note: Colocalizes with CDK5RAP2, CEP152 and WDR62 in a discrete ring around the proximal end of the parental centriole. At this site, a cohesive structure is predicted to engage parental centrioles and procentrioles.
Interacts with CEP152 and CDK1; these interactions recruit both ligands to centrosomes. Interacts with CDK2, CDK5RAP2, WDR62, CEP90, KIAA0753/moonraker and CCDC14. CEP63, CDK5RAP2, CEP152, WDR62 are proposed to form a stepwise assembled complex at the centrosome forming a ring near parental centrioles.
Belongs to the CEP63 family.
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