CEP110 Antibody - #DF9360

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Product: CEP110 Antibody
Catalog: DF9360
Description: Rabbit polyclonal antibody to CEP110
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 113kDa; 113kD(Calculated).
Uniprot: O43303
RRID: AB_2842556

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
CEP110 Antibody detects endogenous levels of total CEP110.
RRID:
AB_2842556
Cite Format: Affinity Biosciences Cat# DF9360, RRID:AB_2842556.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

110 kDa centrosomal protein; CCP110; Centriolar coiled coil protein 110kDa; Centrosomal protein 110 kDa; Centrosomal protein CP110; Centrosomal protein of 110 kDa; Cep110; CP110; CP110_HUMAN; DKFZp781G1416; FAN; KIAA0419;

Immunogens

Immunogen:
Uniprot:

O43303(CP110_HUMAN) >>Visit HPA database.

Gene(ID):
CCP110(9738)
Expression:
O43303 CP110_HUMAN:

Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.

Sequence:
MEEYEKFCEKSLARIQEASLSTESFLPAQSESISLIRFHGVAILSPLLNIEKRKEMQQEKQKALDVEARKQVNRKKALLTRVQEILDNVQVRKAPNASDFDQWEMETVYSNSEVRNLNVPATFPNSFPSHTEHSTAAKLDKIAGILPLDNEDQCKTDGIDLARDSEGFNSPKQCDSSNISHVENEAFPKTSSATPQETLISDGPFSVNEQQDLPLLAEVIPDPYVMSLQNLMKKSKEYIEREQSRRSLRGSINRIVNESHLDKEHDAVEVADCVKEKGQLTGKHCVSVIPDKPSLNKSNVLLQGASTQASSMSMPVLASFSKVDIPIRTGHPTVLESNSDFKVIPTFVTENNVIKSLTGSYAKLPSPEPSMSPKMHRRRSRTSSACHILINNPINACELSPKGKEQAMDLIIQDTDENTNVPEIMPKLPTDLAGVCSSKVYVGKNTSEVKEDVVLGKSNQVCQSSGNHLENKVTHGLVTVEGQLTSDERGAHIMNSTCAAMPKLHEPYASSQCIASPNFGTVSGLKPASMLEKNCSLQTELNKSYDVKNPSPLLMQNQNTRQQMDTPMVSCGNEQFLDNSFEKVKRRLDLDIDGLQKENCPYVITSGITEQERQHLPEKRYPKGSGFVNKNKMLGTSSKESEELLKSKMLAFEEMRKRLEEQHAQQLSLLIAEQEREQERLQKEIEEQEKMLKEKKAMTAEASELDINNAVELEWRKISDSSLLETMLSQADSLHTSNSNSSGFTNSAMQYSFVSANEAPFYLWGSSTSGLTKLSVTRPFGRAKTRWSQVFSLEIQAKFNKITAVAKGFLTRRLMQTDKLKQLRQTVKDTMEFIRSFQSEAPLKRGIVSAQDASLQERVLAQLRAALYGIHDIFFVMDAAERMSILHHDREVRKEKMLRQMDKMKSPRVALSAATQKSLDRKKYMKAAEMGMPNKKFLVKQNPSETRVLQPNQGQNAPVHRLLSRQGTPKTSVKGVVQNRQKPSQSRVPNRVPVSGVYAGKIQRKRPNVATI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Pig
90
Xenopus
70
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43303 As Substrate

Site PTM Type Enzyme
Ubiquitination
K10 Ubiquitination
S19 Phosphorylation
S45 Phosphorylation
S98 Phosphorylation
T107 Phosphorylation
Y109 Phosphorylation
K138 Ubiquitination
K141 Ubiquitination
K155 Ubiquitination
S165 Phosphorylation
S170 Phosphorylation
T194 Phosphorylation
S247 Phosphorylation
S251 Phosphorylation
S259 Phosphorylation
K263 Ubiquitination
K292 Ubiquitination
K342 Ubiquitination
K355 Ubiquitination
S356 Phosphorylation
K363 Ubiquitination
S366 Phosphorylation
S370 Phosphorylation
S372 Phosphorylation
S380 Phosphorylation
T382 Phosphorylation
S383 Phosphorylation
S384 Phosphorylation
S400 Phosphorylation
K439 Ubiquitination
Y441 Phosphorylation
K444 Ubiquitination
K450 Ubiquitination
K457 Ubiquitination
Y508 Phosphorylation
S516 Phosphorylation
K526 Ubiquitination
K533 Ubiquitination
S536 Phosphorylation
K543 Ubiquitination
S544 Phosphorylation
K548 Ubiquitination
S551 Phosphorylation
T566 Phosphorylation
S580 Phosphorylation
K583 Ubiquitination
K597 Ubiquitination
Y602 Phosphorylation
K623 Ubiquitination
S625 Phosphorylation
K630 Ubiquitination
K639 Ubiquitination
S641 Phosphorylation
K648 Ubiquitination
K683 Ubiquitination
K690 Ubiquitination
S788 Phosphorylation
S792 Phosphorylation
K801 Ubiquitination
K819 Ubiquitination
K821 Methylation
K828 Ubiquitination
K844 Ubiquitination
S854 Phosphorylation
K917 Ubiquitination
K926 Ubiquitination
K935 Ubiquitination
K940 Ubiquitination
S995 Phosphorylation
Y998 Phosphorylation
T1011 Phosphorylation

Research Backgrounds

Function:

Necessary for centrosome duplication at different stages of procentriole formation. Acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation. Also involved in promoting ciliogenesis. May play a role in the assembly of the mother centriole subdistal appendages (SDA) thereby effecting the fusion of recycling endosomes to basal bodies during cilia formation (By similarity). Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2.

PTMs:

Phosphorylated by CDKs.

Ubiquitinated by the SCF(CCNF) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication. Deubiquitinated by USP33 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Recruited early and then associates with the growing distal tips. Recruited to the mother centriole by KIF24. Removed from centrioles by TTBK2, leading to initiation of ciliogenesis and localizes only to the daughter centriole in ciliated cells. In cytotoxic T lymphocytes remains associated with the mother centriole during docking of the centrosome at the immunological synapse upon target contact.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.

Subunit Structure:

Interacts with CALM1, CETN2, CEP76, CEP97, CEP104, CEP290, TALPID3. Seems to associate with discrete CETN2, CEP97 and CEP290-containing complexes. Interacts with NEURL4 and CCNF; these interactions are not mutually exclusive and both lead to CCP110 ubiquitination and proteasome-dependent degradation. Via its interaction with NEURL4, may indirectly interact with HERC2. Interacts with KIF24, leading to its recruitment to centrioles. Interacts with USP20 and USP33.

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