Product: CGRRF1 Antibody
Catalog: DF9357
Description: Rabbit polyclonal antibody to CGRRF1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Sheep, Rabbit, Dog
Mol.Wt.: 38 kDa; 38kD(Calculated).
Uniprot: Q99675
RRID: AB_2842553

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Sheep(88%), Rabbit(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
CGRRF1 Antibody detects endogenous levels of total CGRRF1.
RRID:
AB_2842553
Cite Format: Affinity Biosciences Cat# DF9357, RRID:AB_2842553.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cell growth regulator with ring finger domain 1; Cell growth regulator with RING finger domain protein 1; Cell growth regulatory gene 19 protein; Cell growth regulatory with ring finger domain; CGRRF 1; CGRRF1; RING finger protein 197; RNF197;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q99675 CGRF1_HUMAN:

Ubiquitously expressed with high expression in testis and the cerebellum.

Sequence:
MAAVFLVTLYEYSPLFYIAVVFTCFIVTTGLVLGWFGWDVPVILRNSEETQFSTRVFKKQMRQVKNPFGLEITNPSSASITTGITLTTDCLEDSLLTCYWGCSVQKLYEALQKHVYCFRISTPQALEDALYSEYLYQEQYFIKKDSKEEIYCQLPRDTKIEDFGTVPRSRYPLVALLTLADEDDREIYDIISMVSVIHIPDRTYKLSCRILYQYLLLAQGQFHDLKQLFMSANNNFTPSNNSSSEEKNTDRSLLEKVGLSESEVEPSEENSKDCVVCQNGTVNWVLLPCRHTCLCDGCVKYFQQCPMCRQFVQESFALCSQKEQDKDKPKTL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
88
Dog
88
Rabbit
88
Pig
0
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99675 As Substrate

Site PTM Type Enzyme
K147 Ubiquitination
K159 Ubiquitination
S231 Phosphorylation
K247 Ubiquitination
S252 Phosphorylation
K256 Ubiquitination

Research Backgrounds

Function:

Able to inhibit growth in several cell lines.

Subcellular Location:

Nucleus. Endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed with high expression in testis and the cerebellum.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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