BORG5 Antibody - #DF9356

(3)  
Product: BORG5 Antibody
Catalog: DF9356
Description: Rabbit polyclonal antibody to BORG5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 40~50 kDa; 40kD(Calculated).
Uniprot: Q00587
RRID: AB_2842552

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(88%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
BORG5 Antibody detects endogenous levels of total BORG5.
RRID:
AB_2842552
Cite Format: Affinity Biosciences Cat# DF9356, RRID:AB_2842552.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

55 kDa bone marrow stromal/endothelial cell protein; Binder of Rho GTPases 5; Bone marrow stromal/endothelial cell protein, 55-KD; BORG 5; BORG5; BORG5_HUMAN; CDC42 effector protein (Rho GTPase binding) 1; Cdc42 effector protein 1; Cdc42ep1; CEP 1; CEP1; MGC15316; MSE 55; MSE55; OTTHUMP00000028709; Serum constituent protein; Serum protein MSE55;

Immunogens

Immunogen:
Uniprot:

Q00587(BORG5_HUMAN) >>Visit HPA database.

Gene(ID):
CDC42EP1(11135)
Expression:
Q00587 BORG5_HUMAN:

Endothelial and bone marrow stromal cells.

Sequence:
MPGPQGGRGAATMSLGKLSPVGWVSSSQGKRRLTADMISHPLGDFRHTMHVGRGGDVFGDTSFLSNHGGSSGSTHRSPRSFLAKKLQLVRRVGAPPRRMASPPAPSPAPPAISPIIKNAISLPQLNQAAYDSLVVGKLSFDSSPTSSTDGHSSYGLDSGFCTISRLPRSEKPHDRDRDGSFPSEPGLRRSDSLLSFRLDLDLGPSLLSELLGVMSLPEAPAAETPAPAANPPAPTANPTGPAANPPATTANPPAPAANPSAPAATPTGPAANPPAPAASSTPHGHCPNGVTAGLGPVAEVKSSPVGGGPRGPAGPALGRHWGAGWDGGHHYPEMDARQERVEVLPQARASWESLDEEWRAPQAGSRTPVPSTVQANTFEFADAEEDDEVKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Pig
88
Bovine
88
Sheep
88
Rabbit
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q00587 As Substrate

Site PTM Type Enzyme
R8 Methylation
S14 Phosphorylation
K17 Methylation
S19 Phosphorylation
S26 Phosphorylation
S27 Phosphorylation
K30 Ubiquitination
T34 Phosphorylation
R53 Methylation
T61 Phosphorylation
S62 Phosphorylation
S65 Phosphorylation
S70 Phosphorylation
S71 Phosphorylation
S73 Phosphorylation
T74 Phosphorylation
R76 Methylation
S77 Phosphorylation
R79 Methylation
S80 Phosphorylation
R98 Methylation
S101 Phosphorylation
S106 Phosphorylation
S113 Phosphorylation P28482 (MAPK1)
K117 Ubiquitination
S121 Phosphorylation
Y130 Phosphorylation
S132 Phosphorylation
S142 Phosphorylation
S143 Phosphorylation
T145 Phosphorylation
S146 Phosphorylation
S147 Phosphorylation
T148 Phosphorylation
S152 Phosphorylation
Y154 Phosphorylation
S180 Phosphorylation
S183 Phosphorylation
S190 Phosphorylation
S192 Phosphorylation
S195 Phosphorylation P28482 (MAPK1)
S302 Phosphorylation
S303 Phosphorylation
R310 Methylation
Y331 Phosphorylation
S350 Phosphorylation
S353 Phosphorylation
S365 Phosphorylation
T367 Phosphorylation

Research Backgrounds

Function:

Probably involved in the organization of the actin cytoskeleton. Induced membrane extensions in fibroblasts.

Subcellular Location:

Endomembrane system>Peripheral membrane protein. Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Endothelial and bone marrow stromal cells.

Subunit Structure:

Interacts with RHOQ and CDC42, in a GTP-dependent manner.

Family&Domains:

The CRIB domain mediates interaction with CDC42.

Belongs to the BORG/CEP family.

Restrictive clause

 

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